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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4301–4400 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3927 711.3232 1420.6318
3928 711.3232 1420.6318
3929 711.3232 1420.6318
3930 711.3232 1420.6318
3931 711.3232 1420.6318
3932 711.3232 1420.6318
3933 711.3232 1420.6318
3934 711.3232 1420.6318
3935 711.3232 1420.6318
3937 711.3232 1420.6318
3938 711.3232 1420.6318
3939 711.3232 1420.6318
3940 711.3232 1420.6318
3941 711.3232 1420.6318
3942 711.3232 1420.6318
3943 711.3232 1420.6318
3944 711.3232 1420.6318
3945 711.3232 1420.6318
3946 711.3232 1420.6318
3947 711.3232 1420.6318
3948 711.3232 1420.6318
3949 711.3232 1420.6318
3950 711.3232 1420.6318
3952 711.3232 1420.6318
3953 711.3232 1420.6318
3954 711.3232 1420.6318
3955 711.3232 1420.6318
3956 711.3232 1420.6318
3957 711.3232 1420.6318
3958 711.3232 1420.6318
3959 711.3232 1420.6318
3960 711.3232 1420.6318
3961 711.3232 1420.6318
3962 711.3232 1420.6318
3964 711.3232 1420.6318
3965 711.3232 1420.6318
3966 711.3232 1420.6318
3967 711.3232 1420.6318
3968 711.3232 1420.6318
3969 711.3232 1420.6318
3970 711.3232 1420.6318
3972 711.3232 1420.6318
3973 711.3232 1420.6318
3974 711.3232 1420.6318
3975 711.3232 1420.6318
3976 711.3232 1420.6318
3977 711.3232 1420.6318
3978 711.3232 1420.6318
3979 711.3232 1420.6318
3980 711.3232 1420.6318
3981 711.3232 1420.6318
3982 711.3232 1420.6318
3983 711.3232 1420.6318
3985 711.3232 1420.6318
3986 711.3232 1420.6318
3987 711.3232 1420.6318
3988 711.3232 1420.6318
3989 711.3232 1420.6318
3990 711.3232 1420.6318
3991 711.3232 1420.6318
3992 711.3232 1420.6318
3993 711.3232 1420.6318
3994 711.3232 1420.6318
3995 711.3232 1420.6318
3996 711.3232 1420.6318
3997 711.3232 1420.6318
3999 711.3232 1420.6318
4000 711.3232 1420.6318
4001 711.3232 1420.6318
4002 711.3232 1420.6318
4003 711.3232 1420.6318
4004 711.3232 1420.6318
4005 711.3232 1420.6318
4006 711.3232 1420.6318
4007 711.3232 1420.6318
4008 711.3232 1420.6318
4009 711.3232 1420.6318
4010 711.3232 1420.6318
4011 711.3232 1420.6318
4014 711.3232 1420.6318
4015 711.3232 1420.6318
4017 711.3232 1420.6318
4018 711.3232 1420.6318
4019 711.3232 1420.6318
4020 711.3232 1420.6318
4021 711.3232 1420.6318
4022 711.3232 1420.6318
4024 711.3232 1420.6318
4025 711.3232 1420.6318
4026 711.3232 1420.6318
4027 711.3232 1420.6318
4028 711.3232 1420.6318
4029 711.3232 1420.6318
4030 711.3232 1420.6318
4031 711.3232 1420.6318
4032 711.3232 1420.6318
4033 711.3232 1420.6318
4034 711.3232 1420.6318
4035 711.3232 1420.6318
4036 711.3232 1420.6318
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