MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3820 697.8171 1393.6196
3821 697.8171 1393.6196
3822 697.8171 1393.6196
3824 697.8171 1393.6196
3825 697.8171 1393.6196
3826 697.8171 1393.6196
3827 697.8171 1393.6196
3828 697.8171 1393.6196
3829 697.8171 1393.6196
3830 697.8171 1393.6196
3831 697.8171 1393.6196
3832 697.8171 1393.6196
3833 697.8171 1393.6196
3835 697.8171 1393.6196
3836 697.8171 1393.6196
3837 697.8171 1393.6196
3838 697.8171 1393.6196
3839 697.8171 1393.6196
3840 697.8171 1393.6196
3841 697.8171 1393.6196
3842 697.8171 1393.6196
3843 697.8171 1393.6196
3844 697.8171 1393.6196
3845 697.8171 1393.6196
3846 697.8171 1393.6196
3847 697.8171 1393.6196
3848 697.8171 1393.6196
3849 697.8171 1393.6196
3850 697.8171 1393.6196
3851 697.8171 1393.6196
3852 697.8171 1393.6196
3853 697.8171 1393.6196
3854 697.8171 1393.6196
3855 697.8171 1393.6196
3857 697.8171 1393.6196
3858 697.8171 1393.6196
3859 697.8171 1393.6196
3860 697.8171 1393.6196
3861 697.8171 1393.6196
3862 697.8171 1393.6196
3863 697.8171 1393.6196
3864 697.8171 1393.6196
3865 697.8171 1393.6196
3866 697.8171 1393.6196
3867 697.8171 1393.6196
3868 697.8171 1393.6196
3869 697.8171 1393.6196
3872 697.8171 1393.6196
3873 697.8171 1393.6196
3874 697.8171 1393.6196
3875 697.8171 1393.6196
3876 697.8171 1393.6196
3877 697.8171 1393.6196
3878 697.8171 1393.6196
3879 697.8171 1393.6196
3881 697.8171 1393.6196
3882 697.8171 1393.6196
3883 697.8171 1393.6196
3884 697.8171 1393.6196
3885 697.8171 1393.6196
3886 697.8171 1393.6196
3887 697.8171 1393.6196
3888 697.8171 1393.6196
3889 697.8171 1393.6196
3890 697.8171 1393.6196
3891 697.8171 1393.6196
3892 697.8171 1393.6196
3893 697.8171 1393.6196
3894 697.8171 1393.6196
3895 697.8171 1393.6196
3896 697.8171 1393.6196
3897 697.8171 1393.6196
3898 697.8171 1393.6196
3899 697.8171 1393.6196
3900 697.8283 1393.6420
3901 697.8291 1393.6436
3902 697.8293 1393.6440
3903 697.8296 1393.6446
3904 697.8302 1393.6458
3905 697.8305 1393.6464
3906 711.3119 1420.6092
3907 711.3125 1420.6104
3908 711.3127 1420.6108
3909 711.3147 1420.6148
3910 711.3155 1420.6164
3911 711.3199 1420.6252
3912 711.3232 1420.6318
3913 711.3232 1420.6318
3914 711.3232 1420.6318
3915 711.3232 1420.6318
3916 711.3232 1420.6318
3917 711.3232 1420.6318
3918 711.3232 1420.6318
3919 711.3232 1420.6318
3920 711.3232 1420.6318
3921 711.3232 1420.6318
3922 711.3232 1420.6318
3923 711.3232 1420.6318
3925 711.3232 1420.6318
3926 711.3232 1420.6318
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