MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3589 684.3109 1366.6072
3590 684.3109 1366.6072
3591 684.3109 1366.6072
3592 684.3109 1366.6072
3593 684.3109 1366.6072
3594 684.3109 1366.6072
3595 684.3109 1366.6072
3596 684.3109 1366.6072
3597 684.3109 1366.6072
3598 684.3109 1366.6072
3599 684.3109 1366.6072
3600 684.3109 1366.6072
3601 684.3109 1366.6072
3602 684.3109 1366.6072
3603 684.3109 1366.6072
3604 684.3109 1366.6072
3605 684.3109 1366.6072
3606 684.3109 1366.6072
3607 684.3109 1366.6072
3608 684.3109 1366.6072
3609 684.3109 1366.6072
3610 684.3109 1366.6072
3611 684.3109 1366.6072
3612 684.3109 1366.6072
3613 684.3109 1366.6072
3614 684.3109 1366.6072
3615 684.3109 1366.6072
3617 684.3109 1366.6072
3618 684.3109 1366.6072
3619 684.3109 1366.6072
3620 684.3109 1366.6072
3621 684.3109 1366.6072
3622 684.3109 1366.6072
3623 684.3109 1366.6072
3624 684.3109 1366.6072
3628 684.3109 1366.6072
3629 684.3109 1366.6072
3631 684.3109 1366.6072
3632 684.3109 1366.6072
3633 684.3109 1366.6072
3634 684.3109 1366.6072
3635 684.3109 1366.6072
3636 684.3109 1366.6072
3637 684.3109 1366.6072
3638 684.3109 1366.6072
3641 684.3109 1366.6072
3642 684.3109 1366.6072
3643 684.3109 1366.6072
3644 684.3109 1366.6072
3645 684.3109 1366.6072
3647 684.3109 1366.6072
3649 684.3109 1366.6072
3650 684.3109 1366.6072
3651 684.3109 1366.6072
3653 684.3109 1366.6072
3654 684.3109 1366.6072
3655 684.3109 1366.6072
3656 684.3109 1366.6072
3658 684.3109 1366.6072
3659 684.3109 1366.6072
3660 684.3109 1366.6072
3663 684.3109 1366.6072
3664 684.3109 1366.6072
3665 684.3109 1366.6072
3666 684.3109 1366.6072
3667 684.3109 1366.6072
3669 684.3109 1366.6072
3670 684.3109 1366.6072
3671 684.3109 1366.6072
3672 684.3109 1366.6072
3673 684.3109 1366.6072
3674 684.3109 1366.6072
3676 684.3109 1366.6072
3677 684.3109 1366.6072
3678 684.3109 1366.6072
3679 684.3109 1366.6072
3680 684.3109 1366.6072
3681 684.3109 1366.6072
3682 684.3109 1366.6072
3683 684.3109 1366.6072
3684 684.3109 1366.6072
3685 684.3109 1366.6072
3686 684.3109 1366.6072
3688 684.3109 1366.6072
3689 684.3109 1366.6072
3690 684.3109 1366.6072
3691 684.3109 1366.6072
3692 684.3109 1366.6072
3694 684.3109 1366.6072
3695 684.3109 1366.6072
3696 684.3109 1366.6072
3697 684.3109 1366.6072
3698 684.3109 1366.6072
3699 684.3109 1366.6072
3700 684.3109 1366.6072
3701 684.3109 1366.6072
3702 684.3109 1366.6072
3703 684.3109 1366.6072
3704 684.3109 1366.6072
3705 684.3109 1366.6072
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