MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3481 670.8048 1339.5950
3482 670.8048 1339.5950
3484 670.8048 1339.5950
3485 670.8048 1339.5950
3486 670.8048 1339.5950
3487 670.8048 1339.5950
3488 670.8048 1339.5950
3489 670.8048 1339.5950
3490 670.8048 1339.5950
3491 670.8048 1339.5950
3492 670.8048 1339.5950
3494 670.8048 1339.5950
3495 670.8048 1339.5950
3496 670.8048 1339.5950
3497 670.8048 1339.5950
3498 670.8048 1339.5950
3499 670.8048 1339.5950
3500 670.8048 1339.5950
3501 670.8048 1339.5950
3502 670.8048 1339.5950
3503 670.8048 1339.5950
3504 670.8048 1339.5950
3505 670.8048 1339.5950
3506 670.8048 1339.5950
3507 670.8048 1339.5950
3508 670.8048 1339.5950
3509 670.8048 1339.5950
3511 670.8048 1339.5950
3512 670.8048 1339.5950
3513 670.8048 1339.5950
3514 670.8048 1339.5950
3516 670.8048 1339.5950
3517 670.8048 1339.5950
3518 670.8048 1339.5950
3519 670.8048 1339.5950
3520 670.8048 1339.5950
3521 670.8048 1339.5950
3522 670.8048 1339.5950
3523 670.8048 1339.5950
3524 670.8048 1339.5950
3525 670.8048 1339.5950
3526 670.8048 1339.5950
3527 670.8048 1339.5950
3528 670.8048 1339.5950
3529 670.8048 1339.5950
3530 670.8048 1339.5950
3531 670.8048 1339.5950
3532 670.8048 1339.5950
3533 670.8048 1339.5950
3534 670.8048 1339.5950
3535 670.8048 1339.5950
3536 670.8048 1339.5950
3538 670.8048 1339.5950
3539 670.8048 1339.5950
3540 670.8048 1339.5950
3541 670.8048 1339.5950
3542 670.8048 1339.5950
3543 670.8048 1339.5950
3544 670.8048 1339.5950
3545 670.8048 1339.5950
3546 670.8048 1339.5950
3547 670.8048 1339.5950
3548 670.8048 1339.5950
3549 670.8048 1339.5950
3550 670.8048 1339.5950
3551 670.8048 1339.5950
3552 670.8048 1339.5950
3553 670.8048 1339.5950
3554 670.8048 1339.5950
3555 670.8048 1339.5950
3556 670.8048 1339.5950
3557 670.8048 1339.5950
3558 670.8048 1339.5950
3559 670.8048 1339.5950
3560 670.8048 1339.5950
3561 670.8048 1339.5950
3562 670.8048 1339.5950
3564 670.8048 1339.5950
3565 670.8048 1339.5950
3566 670.8048 1339.5950
3567 670.8048 1339.5950
3568 670.8048 1339.5950
3569 670.8048 1339.5950
3570 670.8048 1339.5950
3571 670.8048 1339.5950
3572 670.8048 1339.5950
3573 670.8048 1339.5950
3574 670.8048 1339.5950
3576 670.8048 1339.5950
3577 670.8048 1339.5950
3578 670.8048 1339.5950
3579 670.8053 1339.5960
3580 670.8055 1339.5964
3581 670.8115 1339.6084
3582 684.2975 1366.5804
3583 684.3000 1366.5854
3584 684.3002 1366.5858
3586 684.3055 1366.5964
3587 684.3109 1366.6072
3588 684.3109 1366.6072
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