MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | PRT1377_JBEI_1 |
| MS data file | : | PRT1377_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 23 Jan 2026 at 21:28:41 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 7,307 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 4636 |
765.3478 | 1528.6810 | 1528.6688 | 8.00 | 1 | 3 | 0.75 | 1Score > 15 indicates identity |
VTAGNHCDNLRNR + 3 Deamidated (NQ) |
| 332 |
414.1819 | 826.3492 | 826.3643 | -18.3 | 0 | 3 | 0.54 | 1Score > 13 indicates identity |
YQGMVGR + Deamidated (NQ); Oxidation (M) |
| 666 |
441.1925 | 880.3704 | 880.3562 | 16.1 | 0 | 3 | 0.61 | 1Score > 14 indicates identity |
EDEAFNR + Deamidated (NQ) |
| 3963 |
711.3232 | 1420.6318 | 1420.6512 | -13.6 | 1 | 3 | 1.2 | 1Score > 16 indicates identity |
QMMGMEPKQIAR + 2 Deamidated (NQ) |
| 4130 |
724.8293 | 1447.6440 | 1447.6249 | 13.2 | 0 | 3 | 0.59 | 1Score > 13 indicates identity |
ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M) |
| 6874 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 3 | 1.4 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 4942 |
792.3600 | 1582.7054 | 1582.7110 | -3.53 | 0 | 3 | 0.78 | 1Score > 15 indicates identity |
ALNNELQQEVHQR + 5 Deamidated (NQ) |
| 1275 |
481.7188 | 961.4230 | 961.4215 | 1.59 | 0 | 3 | 0.76 | 1Score > 15 indicates identity |
GSMTFFEK + Oxidation (M) |
| 2232 |
562.7557 | 1123.4968 | 1123.5114 | -13.0 | 0 | 3 | 0.74 | 1Score > 14 indicates identity |
ISGTGMCNIR + Oxidation (M) |
| 2477 |
589.7679 | 1177.5212 | 1177.5363 | -12.8 | 1 | 3 | 0.69 | 1Score > 14 indicates identity |
EGENPYANKR + Deamidated (NQ) |
| 2487 |
589.7679 | 1177.5212 | 1177.5363 | -12.8 | 1 | 3 | 0.69 | 1Score > 14 indicates identity |
EGENPYANKR + Deamidated (NQ) |
| 4834 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 3 | 0.91 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 1023 |
468.2127 | 934.4108 | 934.4219 | -11.8 | 0 | 3 | 0.92 | 1Score > 15 indicates identity |
TFPDCAPK |
| 694 |
441.1938 | 880.3730 | 880.3562 | 19.1 | 0 | 3 | 0.61 | 1Score > 13 indicates identity |
EDEAFNR + Deamidated (NQ) |
| 4106 |
724.8293 | 1447.6440 | 1447.6725 | -19.7 | 1 | 3 | 0.61 | 1Score > 13 indicates identity |
ECAAEITDKAGQR |
| 4185 |
724.8293 | 1447.6440 | 1447.6476 | -2.42 | 0 | 3 | 0.61 | 1Score > 13 indicates identity |
FCQALMTELYR + Deamidated (NQ); Oxidation (M) |
| 971 |
454.7066 | 907.3986 | 907.4035 | -5.38 | 0 | 3 | 0.66 | 1Score > 14 indicates identity |
AQFEEER |
| 2919 |
616.7802 | 1231.5458 | 1231.5615 | -12.7 | 1 | 3 | 0.85 | 1Score > 15 indicates identity |
IREMSDNDPR |
| 3834 |
697.8171 | 1393.6196 | 1393.6474 | -19.9 | 0 | 3 | 1.2 | 1Score > 16 indicates identity |
GDGSLADFGEALSR |
| 2871 |
616.7802 | 1231.5458 | 1231.5615 | -12.7 | 1 | 3 | 0.85 | 1Score > 15 indicates identity |
IREMSDNDPR |
| 1297 |
481.7188 | 961.4230 | 961.4215 | 1.59 | 0 | 3 | 0.78 | 1Score > 15 indicates identity |
GSMTFFEK + Oxidation (M) |
| 2046 |
549.2496 | 1096.4846 | 1096.4753 | 8.49 | 1 | 3 | 0.83 | 1Score > 15 indicates identity |
GDARMMASSR + Oxidation (M) |
| 2362 |
576.2618 | 1150.5090 | 1150.4891 | 17.4 | 0 | 3 | 0.91 | 1Score > 15 indicates identity |
DEAQEQQFR + Deamidated (NQ) |
| 5515 |
832.8785 | 1663.7424 | 1663.7723 | -17.9 | 0 | 3 | 1 | 1Score > 16 indicates identity |
MTQQGDAVAGELATEK + Oxidation (M) |
| 2326 |
576.2618 | 1150.5090 | 1150.5254 | -14.2 | 1 | 3 | 0.91 | 1Score > 15 indicates identity |
LEFNNDNRK + 2 Deamidated (NQ) |
| 6232 |
900.4092 | 1798.8038 | 1798.8018 | 1.13 | 1 | 3 | 0.86 | 1Score > 15 indicates identity |
NVFMGKLNDMVNWGR + 3 Deamidated (NQ); Oxidation (M) |
| 1292 |
481.7188 | 961.4230 | 961.4215 | 1.59 | 0 | 3 | 0.79 | 1Score > 15 indicates identity |
GSMTFFEK + Oxidation (M) |
| 6312 |
900.4092 | 1798.8038 | 1798.7870 | 9.35 | 0 | 3 | 0.87 | 1Score > 15 indicates identity |
TQADAWDINQGHQSAR + 2 Deamidated (NQ) |
| 6868 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 3 | 1.4 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 5910 |
873.3969 | 1744.7792 | 1744.8057 | -15.1 | 1 | 3 | 1.7 | 1Score > 18 indicates identity |
QGDFKSPEGFYSVQR + Deamidated (NQ) |
| 6279 |
900.4092 | 1798.8038 | 1798.8308 | -15.0 | 0 | 3 | 0.87 | 1Score > 15 indicates identity |
IYDSDSILSMHHEPR |
| 486 |
414.1881 | 826.3616 | 826.3491 | 15.2 | 0 | 3 | 0.57 | 1Score > 13 indicates identity |
MTESTSR + Oxidation (M) |
| 6437 |
913.9153 | 1825.8160 | 1825.8482 | -17.6 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
EDAQLLYGFNNKQER + 2 Deamidated (NQ) |
| 1298 |
481.7188 | 961.4230 | 961.4215 | 1.59 | 0 | 3 | 0.8 | 1Score > 15 indicates identity |
GSMTFFEK + Oxidation (M) |
| 3338 |
657.2986 | 1312.5826 | 1312.5908 | -6.24 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
HGNRGNWEASGK + Deamidated (NQ) |
| 6895 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 3 | 1.5 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 962 |
454.7066 | 907.3986 | 907.4035 | -5.38 | 0 | 3 | 0.68 | 1Score > 14 indicates identity |
AQFEEER |
| 6324 |
900.4092 | 1798.8038 | 1798.8308 | -15.0 | 0 | 3 | 0.89 | 1Score > 15 indicates identity |
IYDSDSILSMHHEPR |
| 6721 |
940.9276 | 1879.8406 | 1879.8305 | 5.41 | 1 | 3 | 1.5 | 1Score > 17 indicates identity |
QSHGNPLMKQGHQMQR + 4 Deamidated (NQ) |
| 4494 |
751.8416 | 1501.6686 | 1501.6772 | -5.69 | 0 | 3 | 1.4 | 1Score > 17 indicates identity |
YHQWMNELPER |
| 4821 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 3 | 0.97 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 5349 |
819.3723 | 1636.7300 | 1636.7403 | -6.24 | 0 | 3 | 1.3 | 1Score > 16 indicates identity |
GFNQSDLLCQPLSR + 3 Deamidated (NQ) |
| 2392 |
576.2618 | 1150.5090 | 1150.4933 | 13.7 | 0 | 3 | 0.95 | 1Score > 15 indicates identity |
CMTQMNIPR + Deamidated (NQ) |
| 3871 |
697.8171 | 1393.6196 | 1393.6329 | -9.53 | 1 | 3 | 1.2 | 1Score > 16 indicates identity |
EEMATEMERLR |
| 4432 |
751.8416 | 1501.6686 | 1501.6943 | -17.1 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
EKMQTNPQNQQR + Deamidated (NQ) |
| 6258 |
900.4092 | 1798.8038 | 1798.8308 | -15.0 | 0 | 3 | 0.9 | 1Score > 15 indicates identity |
IYDSDSILSMHHEPR |
| 115 |
387.1806 | 772.3466 | 772.3351 | 14.9 | 0 | 3 | 0.52 | 1Score > 13 indicates identity |
DPNGDVR + Deamidated (NQ) |
| 6277 |
900.4092 | 1798.8038 | 1798.8308 | -15.0 | 0 | 3 | 0.91 | 1Score > 15 indicates identity |
IYDSDSILSMHHEPR |
| 2024 |
549.2496 | 1096.4846 | 1096.4753 | 8.49 | 1 | 3 | 0.89 | 1Score > 15 indicates identity |
GDARMMASSR + Oxidation (M) |
| 5735 |
859.8907 | 1717.7668 | 1717.7875 | -12.0 | 1 | 3 | 0.97 | 1Score > 15 indicates identity |
AEDMAAANAMASHLRK + 2 Oxidation (M) |
| 6227 |
900.4092 | 1798.8038 | 1798.7953 | 4.76 | 0 | 3 | 0.92 | 1Score > 15 indicates identity |
CLNHMFAAEIYMQR + Oxidation (M) |
| 6403 |
913.9153 | 1825.8160 | 1825.8087 | 4.02 | 1 | 3 | 1.5 | 1Score > 17 indicates identity |
NGMECGIGVKNYNDVR + Deamidated (NQ) |
| 4851 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 3 | 1 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 5341 |
819.3723 | 1636.7300 | 1636.7371 | -4.31 | 1 | 3 | 1.3 | 1Score > 16 indicates identity |
CVELVREMMGEQR + Deamidated (NQ) |
| 7084 |
967.9399 | 1933.8652 | 1933.8298 | 18.3 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
QSMGFDTNMEGGFLARR + 2 Deamidated (NQ); Oxidation (M) |
| 1699 |
522.2372 | 1042.4598 | 1042.4614 | -1.50 | 0 | 3 | 0.53 | 1Score > 13 indicates identity |
QQMEGHASR |
| 3212 |
643.7925 | 1285.5704 | 1285.5649 | 4.35 | 0 | 3 | 0.88 | 1Score > 15 indicates identity |
QAMWYINSQK + 2 Deamidated (NQ); Oxidation (M) |
| 3736 |
684.3109 | 1366.6072 | 1366.5823 | 18.3 | 1 | 3 | 1.2 | 1Score > 16 indicates identity |
MNTRWQQAGQK + 4 Deamidated (NQ); Oxidation (M) |
| 5036 |
792.3600 | 1582.7054 | 1582.6875 | 11.4 | 0 | 3 | 0.88 | 1Score > 15 indicates identity |
VYFCDPQSPWQR + Deamidated (NQ) |
| 4302 |
738.3355 | 1474.6564 | 1474.6722 | -10.7 | 1 | 3 | 1 | 1Score > 15 indicates identity |
DQSHLSMEERVK + Deamidated (NQ); Oxidation (M) |
| 4451 |
751.8416 | 1501.6686 | 1501.6871 | -12.3 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
MNKTEFYADLNR + Deamidated (NQ) |
| 6331 |
900.4092 | 1798.8038 | 1798.7870 | 9.35 | 0 | 3 | 0.94 | 1Score > 15 indicates identity |
TQADAWDINQGHQSAR + 2 Deamidated (NQ) |
| 1840 |
535.7434 | 1069.4722 | 1069.4862 | -13.1 | 0 | 3 | 0.54 | 1Score > 13 indicates identity |
YCVNSASLR + Deamidated (NQ) |
| 3084 |
630.2864 | 1258.5582 | 1258.5724 | -11.3 | 1 | 3 | 0.73 | 1Score > 14 indicates identity |
VNDRSHQMEK + Oxidation (M) |
| 6872 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 3 | 1.6 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 6550 |
927.4214 | 1852.8282 | 1852.8389 | -5.74 | 0 | 3 | 1.4 | 1Score > 17 indicates identity |
SLPWQAVWEMYCQR |
| 6876 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 3 | 1.6 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 4981 |
792.3600 | 1582.7054 | 1582.6875 | 11.4 | 0 | 3 | 0.89 | 1Score > 15 indicates identity |
VYFCDPQSPWQR + Deamidated (NQ) |
| 3179 |
643.7925 | 1285.5704 | 1285.5761 | -4.41 | 0 | 3 | 0.89 | 1Score > 15 indicates identity |
MQAYFDQLDR |
| 434 |
414.1881 | 826.3616 | 826.3677 | -7.33 | 0 | 3 | 0.62 | 1Score > 13 indicates identity |
MATVSMR + 2 Oxidation (M) |
| 6286 |
900.4092 | 1798.8038 | 1798.8155 | -6.50 | 0 | 3 | 0.95 | 1Score > 15 indicates identity |
ASAEEIQDHQCEILR + Deamidated (NQ) |
| 6326 |
900.4092 | 1798.8038 | 1798.7870 | 9.35 | 0 | 3 | 0.95 | 1Score > 15 indicates identity |
TQADAWDINQGHQSAR + 2 Deamidated (NQ) |
| 6320 |
900.4092 | 1798.8038 | 1798.7870 | 9.35 | 0 | 3 | 0.95 | 1Score > 15 indicates identity |
TQADAWDINQGHQSAR + 2 Deamidated (NQ) |
| 4940 |
792.3600 | 1582.7054 | 1582.7110 | -3.53 | 0 | 3 | 0.9 | 1Score > 15 indicates identity |
ALNNELQQEVHQR + 5 Deamidated (NQ) |
| 1683 |
522.2372 | 1042.4598 | 1042.4576 | 2.17 | 0 | 3 | 0.55 | 1Score > 13 indicates identity |
GFADAIMMR + 2 Oxidation (M) |
| 4109 |
724.8293 | 1447.6440 | 1447.6725 | -19.7 | 1 | 3 | 0.68 | 1Score > 13 indicates identity |
ECAAEITDKAGQR |
| 4440 |
751.8416 | 1501.6686 | 1501.6943 | -17.1 | 1 | 3 | 1.5 | 1Score > 17 indicates identity |
EKMQTNPQNQQR + Deamidated (NQ) |
| 6849 |
954.4337 | 1906.8528 | 1906.8189 | 17.8 | 0 | 3 | 1.6 | 1Score > 17 indicates identity |
AMSDLQTPEWVGADCAR + Deamidated (NQ) |
| 1638 |
522.2357 | 1042.4568 | 1042.4576 | -0.70 | 0 | 3 | 0.55 | 1Score > 13 indicates identity |
GFADAIMMR + 2 Oxidation (M) |
| 6546 |
927.4214 | 1852.8282 | 1852.8481 | -10.7 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
MGVRESLEEQTMNMAK |
| 3336 |
657.2986 | 1312.5826 | 1312.6081 | -19.4 | 1 | 3 | 1.2 | 1Score > 16 indicates identity |
AGGTIMFSNNKR + 2 Deamidated (NQ); Oxidation (M) |
| 1030 |
468.2127 | 934.4108 | 934.4178 | -7.46 | 1 | 3 | 1 | 1Score > 15 indicates identity |
LDEDCKR |
| 7080 |
967.9399 | 1933.8652 | 1933.8298 | 18.3 | 1 | 3 | 1.5 | 1Score > 17 indicates identity |
QSMGFDTNMEGGFLARR + 2 Deamidated (NQ); Oxidation (M) |
| 1818 |
535.7434 | 1069.4722 | 1069.4862 | -13.1 | 0 | 3 | 0.55 | 1Score > 13 indicates identity |
YCVNSASLR + Deamidated (NQ) |
| 4338 |
738.3355 | 1474.6564 | 1474.6722 | -10.7 | 1 | 3 | 1.1 | 1Score > 15 indicates identity |
DQSHLSMEERVK + Deamidated (NQ); Oxidation (M) |
| 1191 |
481.7188 | 961.4230 | 961.4253 | -2.38 | 0 | 3 | 0.89 | 1Score > 15 indicates identity |
SGNDLHYR + Deamidated (NQ) |
| 1816 |
535.7434 | 1069.4722 | 1069.4862 | -13.1 | 0 | 3 | 0.56 | 1Score > 13 indicates identity |
YCVNSASLR + Deamidated (NQ) |
| 815 |
441.2092 | 880.4038 | 880.4081 | -4.84 | 1 | 3 | 1.5 | 1Score > 17 indicates identity |
GGMAKMMR |
| 2021 |
549.2496 | 1096.4846 | 1096.4753 | 8.49 | 1 | 3 | 0.95 | 1Score > 15 indicates identity |
GDARMMASSR + Oxidation (M) |
| 2401 |
576.2618 | 1150.5090 | 1150.5288 | -17.2 | 0 | 3 | 1 | 1Score > 15 indicates identity |
QTQLTEEMR + Oxidation (M) |
| 4635 |
765.3478 | 1528.6810 | 1528.7005 | -12.7 | 0 | 3 | 0.9 | 1Score > 15 indicates identity |
DLSALEDAAEDQPR |
| 5511 |
832.8785 | 1663.7424 | 1663.7157 | 16.1 | 0 | 3 | 1.2 | 1Score > 16 indicates identity |
VPPGMTMMYHAQER + Deamidated (NQ); Oxidation (M) |
| 1101 |
468.2127 | 934.4108 | 934.4178 | -7.46 | 1 | 2 | 1.1 | 1Score > 15 indicates identity |
LDEDCKR |
| 77 |
387.1795 | 772.3444 | 772.3351 | 12.0 | 0 | 2 | 0.57 | 1Score > 13 indicates identity |
DPNGDVR + Deamidated (NQ) |
| 1040 |
468.2127 | 934.4108 | 934.4178 | -7.46 | 1 | 2 | 1.1 | 1Score > 15 indicates identity |
LDEDCKR |
| 4016 |
711.3232 | 1420.6318 | 1420.6201 | 8.29 | 1 | 2 | 1.4 | 1Score > 16 indicates identity |
HSQTHCAHCRK |
| 6005 |
873.4037 | 1744.7928 | 1744.7976 | -2.73 | 0 | 2 | 2.3 | 1Score > 19 indicates identity |
GTAVNSDGASNGLSAPNGR + Deamidated (NQ) |
| 6166 |
886.9030 | 1771.7914 | 1771.7968 | -3.04 | 0 | 2 | 1.3 | 1Score > 16 indicates identity |
SQSTVGAGDSMVGAMTLK + Deamidated (NQ); 2 Oxidation (M) |
| 2656 |
603.2741 | 1204.5336 | 1204.5394 | -4.75 | 0 | 2 | 1.3 | 1Score > 16 indicates identity |
ADCEQLAELR + Deamidated (NQ) |
| 1404 |
495.2244 | 988.4342 | 988.4324 | 1.87 | 0 | 2 | 0.58 | 1Score > 13 indicates identity |
MDYEFLR + Oxidation (M) |
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