MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3374 657.2986 1312.5826
3375 657.2986 1312.5826
3376 657.2986 1312.5826
3377 657.2986 1312.5826
3378 657.2986 1312.5826
3379 657.2986 1312.5826
3380 657.2986 1312.5826
3381 657.2986 1312.5826
3382 657.2986 1312.5826
3384 657.2986 1312.5826
3385 657.2986 1312.5826
3386 657.2986 1312.5826
3387 657.2986 1312.5826
3388 657.2986 1312.5826
3389 657.2986 1312.5826
3390 657.2986 1312.5826
3391 657.2986 1312.5826
3393 657.2986 1312.5826
3394 657.2986 1312.5826
3395 657.2986 1312.5826
3396 657.2986 1312.5826
3397 657.2986 1312.5826
3398 657.2986 1312.5826
3399 657.2986 1312.5826
3400 657.2986 1312.5826
3401 657.2986 1312.5826
3402 657.2986 1312.5826
3403 657.2986 1312.5826
3404 657.2986 1312.5826
3406 657.2986 1312.5826
3407 657.2986 1312.5826
3408 657.2986 1312.5826
3409 657.2986 1312.5826
3410 657.2986 1312.5826
3411 657.2986 1312.5826
3412 657.2986 1312.5826
3413 657.2986 1312.5826
3414 657.2990 1312.5834
3415 657.3031 1312.5916
3416 657.3059 1312.5972
3417 657.3085 1312.6024
3418 657.3094 1312.6042
3419 657.3107 1312.6068
3420 670.8043 1339.5940
3421 670.8048 1339.5950
3422 670.8048 1339.5950
3423 670.8048 1339.5950
3424 670.8048 1339.5950
3425 670.8048 1339.5950
3426 670.8048 1339.5950
3427 670.8048 1339.5950
3428 670.8048 1339.5950
3429 670.8048 1339.5950
3430 670.8048 1339.5950
3431 670.8048 1339.5950
3433 670.8048 1339.5950
3434 670.8048 1339.5950
3435 670.8048 1339.5950
3436 670.8048 1339.5950
3437 670.8048 1339.5950
3438 670.8048 1339.5950
3439 670.8048 1339.5950
3440 670.8048 1339.5950
3441 670.8048 1339.5950
3442 670.8048 1339.5950
3443 670.8048 1339.5950
3444 670.8048 1339.5950
3445 670.8048 1339.5950
3446 670.8048 1339.5950
3447 670.8048 1339.5950
3448 670.8048 1339.5950
3449 670.8048 1339.5950
3450 670.8048 1339.5950
3451 670.8048 1339.5950
3453 670.8048 1339.5950
3454 670.8048 1339.5950
3455 670.8048 1339.5950
3456 670.8048 1339.5950
3457 670.8048 1339.5950
3458 670.8048 1339.5950
3459 670.8048 1339.5950
3460 670.8048 1339.5950
3461 670.8048 1339.5950
3462 670.8048 1339.5950
3463 670.8048 1339.5950
3464 670.8048 1339.5950
3465 670.8048 1339.5950
3466 670.8048 1339.5950
3467 670.8048 1339.5950
3468 670.8048 1339.5950
3469 670.8048 1339.5950
3470 670.8048 1339.5950
3471 670.8048 1339.5950
3472 670.8048 1339.5950
3473 670.8048 1339.5950
3474 670.8048 1339.5950
3476 670.8048 1339.5950
3477 670.8048 1339.5950
3478 670.8048 1339.5950
3479 670.8048 1339.5950
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