MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3051 630.2864 1258.5582
3052 630.2864 1258.5582
3053 630.2864 1258.5582
3054 630.2864 1258.5582
3055 630.2864 1258.5582
3056 630.2864 1258.5582
3058 630.2864 1258.5582
3059 630.2864 1258.5582
3060 630.2864 1258.5582
3061 630.2864 1258.5582
3062 630.2864 1258.5582
3063 630.2864 1258.5582
3064 630.2864 1258.5582
3065 630.2864 1258.5582
3066 630.2864 1258.5582
3067 630.2864 1258.5582
3068 630.2864 1258.5582
3069 630.2864 1258.5582
3070 630.2864 1258.5582
3071 630.2864 1258.5582
3072 630.2864 1258.5582
3073 630.2864 1258.5582
3074 630.2864 1258.5582
3075 630.2864 1258.5582
3076 630.2864 1258.5582
3077 630.2864 1258.5582
3078 630.2864 1258.5582
3079 630.2864 1258.5582
3080 630.2864 1258.5582
3081 630.2864 1258.5582
3082 630.2864 1258.5582
3083 630.2864 1258.5582
3085 630.2864 1258.5582
3086 630.2864 1258.5582
3088 630.2864 1258.5582
3089 630.2864 1258.5582
3090 630.2864 1258.5582
3091 630.2864 1258.5582
3092 630.2864 1258.5582
3093 630.2872 1258.5598
3094 630.2944 1258.5742
3095 630.2977 1258.5808
3096 643.7925 1285.5704
3097 643.7925 1285.5704
3098 643.7925 1285.5704
3099 643.7925 1285.5704
3100 643.7925 1285.5704
3101 643.7925 1285.5704
3102 643.7925 1285.5704
3103 643.7925 1285.5704
3104 643.7925 1285.5704
3105 643.7925 1285.5704
3106 643.7925 1285.5704
3107 643.7925 1285.5704
3108 643.7925 1285.5704
3109 643.7925 1285.5704
3110 643.7925 1285.5704
3111 643.7925 1285.5704
3112 643.7925 1285.5704
3113 643.7925 1285.5704
3114 643.7925 1285.5704
3115 643.7925 1285.5704
3116 643.7925 1285.5704
3117 643.7925 1285.5704
3118 643.7925 1285.5704
3119 643.7925 1285.5704
3120 643.7925 1285.5704
3121 643.7925 1285.5704
3122 643.7925 1285.5704
3123 643.7925 1285.5704
3125 643.7925 1285.5704
3126 643.7925 1285.5704
3127 643.7925 1285.5704
3128 643.7925 1285.5704
3129 643.7925 1285.5704
3130 643.7925 1285.5704
3131 643.7925 1285.5704
3132 643.7925 1285.5704
3133 643.7925 1285.5704
3135 643.7925 1285.5704
3136 643.7925 1285.5704
3137 643.7925 1285.5704
3138 643.7925 1285.5704
3139 643.7925 1285.5704
3140 643.7925 1285.5704
3141 643.7925 1285.5704
3142 643.7925 1285.5704
3143 643.7925 1285.5704
3144 643.7925 1285.5704
3145 643.7925 1285.5704
3146 643.7925 1285.5704
3147 643.7925 1285.5704
3149 643.7925 1285.5704
3150 643.7925 1285.5704
3151 643.7925 1285.5704
3152 643.7925 1285.5704
3153 643.7925 1285.5704
3154 643.7925 1285.5704
3156 643.7925 1285.5704
3158 643.7925 1285.5704
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