MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2945 630.2837 1258.5528
2946 630.2839 1258.5532
2947 630.2859 1258.5572
2948 630.2864 1258.5582
2949 630.2864 1258.5582
2950 630.2864 1258.5582
2951 630.2864 1258.5582
2952 630.2864 1258.5582
2953 630.2864 1258.5582
2954 630.2864 1258.5582
2955 630.2864 1258.5582
2956 630.2864 1258.5582
2957 630.2864 1258.5582
2958 630.2864 1258.5582
2960 630.2864 1258.5582
2961 630.2864 1258.5582
2962 630.2864 1258.5582
2963 630.2864 1258.5582
2964 630.2864 1258.5582
2966 630.2864 1258.5582
2967 630.2864 1258.5582
2968 630.2864 1258.5582
2969 630.2864 1258.5582
2970 630.2864 1258.5582
2971 630.2864 1258.5582
2972 630.2864 1258.5582
2973 630.2864 1258.5582
2974 630.2864 1258.5582
2977 630.2864 1258.5582
2978 630.2864 1258.5582
2979 630.2864 1258.5582
2980 630.2864 1258.5582
2981 630.2864 1258.5582
2982 630.2864 1258.5582
2983 630.2864 1258.5582
2984 630.2864 1258.5582
2985 630.2864 1258.5582
2986 630.2864 1258.5582
2987 630.2864 1258.5582
2988 630.2864 1258.5582
2989 630.2864 1258.5582
2990 630.2864 1258.5582
2991 630.2864 1258.5582
2992 630.2864 1258.5582
2993 630.2864 1258.5582
2994 630.2864 1258.5582
2995 630.2864 1258.5582
2996 630.2864 1258.5582
2997 630.2864 1258.5582
2998 630.2864 1258.5582
2999 630.2864 1258.5582
3000 630.2864 1258.5582
3001 630.2864 1258.5582
3002 630.2864 1258.5582
3003 630.2864 1258.5582
3004 630.2864 1258.5582
3005 630.2864 1258.5582
3006 630.2864 1258.5582
3007 630.2864 1258.5582
3008 630.2864 1258.5582
3009 630.2864 1258.5582
3010 630.2864 1258.5582
3011 630.2864 1258.5582
3012 630.2864 1258.5582
3013 630.2864 1258.5582
3014 630.2864 1258.5582
3015 630.2864 1258.5582
3016 630.2864 1258.5582
3017 630.2864 1258.5582
3018 630.2864 1258.5582
3019 630.2864 1258.5582
3020 630.2864 1258.5582
3021 630.2864 1258.5582
3023 630.2864 1258.5582
3024 630.2864 1258.5582
3025 630.2864 1258.5582
3026 630.2864 1258.5582
3027 630.2864 1258.5582
3028 630.2864 1258.5582
3029 630.2864 1258.5582
3030 630.2864 1258.5582
3031 630.2864 1258.5582
3032 630.2864 1258.5582
3033 630.2864 1258.5582
3034 630.2864 1258.5582
3035 630.2864 1258.5582
3036 630.2864 1258.5582
3037 630.2864 1258.5582
3038 630.2864 1258.5582
3039 630.2864 1258.5582
3040 630.2864 1258.5582
3041 630.2864 1258.5582
3042 630.2864 1258.5582
3043 630.2864 1258.5582
3044 630.2864 1258.5582
3045 630.2864 1258.5582
3047 630.2864 1258.5582
3048 630.2864 1258.5582
3049 630.2864 1258.5582
3050 630.2864 1258.5582
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