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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2831 616.7802 1231.5458
2833 616.7802 1231.5458
2834 616.7802 1231.5458
2835 616.7802 1231.5458
2836 616.7802 1231.5458
2837 616.7802 1231.5458
2838 616.7802 1231.5458
2839 616.7802 1231.5458
2840 616.7802 1231.5458
2841 616.7802 1231.5458
2842 616.7802 1231.5458
2843 616.7802 1231.5458
2844 616.7802 1231.5458
2845 616.7802 1231.5458
2847 616.7802 1231.5458
2848 616.7802 1231.5458
2849 616.7802 1231.5458
2850 616.7802 1231.5458
2851 616.7802 1231.5458
2853 616.7802 1231.5458
2856 616.7802 1231.5458
2858 616.7802 1231.5458
2859 616.7802 1231.5458
2860 616.7802 1231.5458
2861 616.7802 1231.5458
2862 616.7802 1231.5458
2863 616.7802 1231.5458
2864 616.7802 1231.5458
2865 616.7802 1231.5458
2866 616.7802 1231.5458
2867 616.7802 1231.5458
2868 616.7802 1231.5458
2869 616.7802 1231.5458
2870 616.7802 1231.5458
2872 616.7802 1231.5458
2873 616.7802 1231.5458
2874 616.7802 1231.5458
2875 616.7802 1231.5458
2876 616.7802 1231.5458
2880 616.7802 1231.5458
2881 616.7802 1231.5458
2882 616.7802 1231.5458
2883 616.7802 1231.5458
2884 616.7802 1231.5458
2885 616.7802 1231.5458
2886 616.7802 1231.5458
2887 616.7802 1231.5458
2889 616.7802 1231.5458
2890 616.7802 1231.5458
2891 616.7802 1231.5458
2892 616.7802 1231.5458
2894 616.7802 1231.5458
2895 616.7802 1231.5458
2897 616.7802 1231.5458
2898 616.7802 1231.5458
2899 616.7802 1231.5458
2900 616.7802 1231.5458
2901 616.7802 1231.5458
2902 616.7802 1231.5458
2903 616.7802 1231.5458
2904 616.7802 1231.5458
2905 616.7802 1231.5458
2906 616.7802 1231.5458
2907 616.7802 1231.5458
2908 616.7802 1231.5458
2909 616.7802 1231.5458
2910 616.7802 1231.5458
2911 616.7802 1231.5458
2912 616.7802 1231.5458
2913 616.7802 1231.5458
2914 616.7802 1231.5458
2915 616.7802 1231.5458
2916 616.7802 1231.5458
2917 616.7802 1231.5458
2918 616.7802 1231.5458
2920 616.7802 1231.5458
2921 616.7802 1231.5458
2922 616.7802 1231.5458
2923 616.7802 1231.5458
2924 616.7802 1231.5458
2925 616.7802 1231.5458
2926 616.7802 1231.5458
2927 616.7802 1231.5458
2928 616.7802 1231.5458
2929 616.7802 1231.5458
2930 616.7802 1231.5458
2931 616.7802 1231.5458
2932 616.7914 1231.5682
2933 616.7924 1231.5702
2934 630.2792 1258.5438
2935 630.2800 1258.5454
2936 630.2802 1258.5458
2937 630.2806 1258.5466
2938 630.2814 1258.5482
2939 630.2814 1258.5482
2940 630.2814 1258.5482
2941 630.2822 1258.5498
2942 630.2830 1258.5514
2943 630.2831 1258.5516
2944 630.2833 1258.5520
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