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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2716 603.2741 1204.5336
2718 603.2741 1204.5336
2719 603.2741 1204.5336
2720 603.2741 1204.5336
2721 603.2741 1204.5336
2722 603.2741 1204.5336
2723 603.2741 1204.5336
2724 603.2741 1204.5336
2725 603.2741 1204.5336
2726 603.2741 1204.5336
2727 603.2741 1204.5336
2728 603.2741 1204.5336
2729 603.2741 1204.5336
2730 603.2741 1204.5336
2731 603.2741 1204.5336
2732 603.2741 1204.5336
2733 603.2741 1204.5336
2735 603.2741 1204.5336
2736 603.2741 1204.5336
2737 603.2741 1204.5336
2738 603.2741 1204.5336
2739 603.2741 1204.5336
2740 603.2741 1204.5336
2741 603.2741 1204.5336
2743 603.2741 1204.5336
2744 603.2741 1204.5336
2745 603.2741 1204.5336
2746 603.2741 1204.5336
2747 603.2741 1204.5336
2748 603.2741 1204.5336
2750 603.2741 1204.5336
2751 603.2741 1204.5336
2753 603.2741 1204.5336
2754 603.2741 1204.5336
2755 603.2741 1204.5336
2756 603.2741 1204.5336
2757 603.2741 1204.5336
2758 603.2741 1204.5336
2759 603.2741 1204.5336
2760 603.2741 1204.5336
2761 603.2798 1204.5450
2763 603.2803 1204.5460
2764 603.2811 1204.5476
2765 603.2816 1204.5486
2766 603.2828 1204.5510
2768 603.2838 1204.5530
2770 603.2844 1204.5542
2771 603.2848 1204.5550
2772 616.7802 1231.5458
2773 616.7802 1231.5458
2774 616.7802 1231.5458
2775 616.7802 1231.5458
2777 616.7802 1231.5458
2778 616.7802 1231.5458
2779 616.7802 1231.5458
2780 616.7802 1231.5458
2781 616.7802 1231.5458
2782 616.7802 1231.5458
2783 616.7802 1231.5458
2784 616.7802 1231.5458
2785 616.7802 1231.5458
2786 616.7802 1231.5458
2787 616.7802 1231.5458
2788 616.7802 1231.5458
2789 616.7802 1231.5458
2790 616.7802 1231.5458
2791 616.7802 1231.5458
2792 616.7802 1231.5458
2793 616.7802 1231.5458
2794 616.7802 1231.5458
2795 616.7802 1231.5458
2796 616.7802 1231.5458
2797 616.7802 1231.5458
2798 616.7802 1231.5458
2799 616.7802 1231.5458
2800 616.7802 1231.5458
2801 616.7802 1231.5458
2802 616.7802 1231.5458
2804 616.7802 1231.5458
2805 616.7802 1231.5458
2806 616.7802 1231.5458
2808 616.7802 1231.5458
2809 616.7802 1231.5458
2811 616.7802 1231.5458
2812 616.7802 1231.5458
2813 616.7802 1231.5458
2814 616.7802 1231.5458
2815 616.7802 1231.5458
2816 616.7802 1231.5458
2818 616.7802 1231.5458
2820 616.7802 1231.5458
2821 616.7802 1231.5458
2822 616.7802 1231.5458
2823 616.7802 1231.5458
2824 616.7802 1231.5458
2825 616.7802 1231.5458
2826 616.7802 1231.5458
2827 616.7802 1231.5458
2828 616.7802 1231.5458
2829 616.7802 1231.5458
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