MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2390 576.2618 1150.5090
2391 576.2618 1150.5090
2393 576.2618 1150.5090
2394 576.2618 1150.5090
2395 576.2618 1150.5090
2396 576.2618 1150.5090
2397 576.2618 1150.5090
2398 576.2618 1150.5090
2399 576.2618 1150.5090
2402 576.2618 1150.5090
2403 576.2618 1150.5090
2404 576.2618 1150.5090
2405 576.2618 1150.5090
2406 576.2618 1150.5090
2407 576.2618 1150.5090
2408 576.2618 1150.5090
2409 576.2618 1150.5090
2410 576.2618 1150.5090
2411 576.2618 1150.5090
2412 576.2618 1150.5090
2413 576.2618 1150.5090
2414 576.2618 1150.5090
2415 576.2618 1150.5090
2416 576.2618 1150.5090
2417 576.2618 1150.5090
2418 576.2618 1150.5090
2419 576.2618 1150.5090
2420 576.2618 1150.5090
2422 576.2618 1150.5090
2423 576.2618 1150.5090
2424 576.2618 1150.5090
2425 576.2618 1150.5090
2426 576.2618 1150.5090
2427 576.2618 1150.5090
2429 576.2618 1150.5090
2430 576.2618 1150.5090
2431 576.2618 1150.5090
2433 576.2618 1150.5090
2434 576.2618 1150.5090
2435 576.2618 1150.5090
2436 576.2618 1150.5090
2437 576.2618 1150.5090
2438 576.2618 1150.5090
2439 576.2618 1150.5090
2440 576.2618 1150.5090
2441 576.2618 1150.5090
2442 576.2618 1150.5090
2443 576.2618 1150.5090
2444 576.2618 1150.5090
2445 576.2694 1150.5242
2446 589.7679 1177.5212
2447 589.7679 1177.5212
2448 589.7679 1177.5212
2449 589.7679 1177.5212
2450 589.7679 1177.5212
2451 589.7679 1177.5212
2452 589.7679 1177.5212
2453 589.7679 1177.5212
2454 589.7679 1177.5212
2455 589.7679 1177.5212
2456 589.7679 1177.5212
2457 589.7679 1177.5212
2458 589.7679 1177.5212
2459 589.7679 1177.5212
2460 589.7679 1177.5212
2461 589.7679 1177.5212
2462 589.7679 1177.5212
2463 589.7679 1177.5212
2464 589.7679 1177.5212
2465 589.7679 1177.5212
2466 589.7679 1177.5212
2467 589.7679 1177.5212
2468 589.7679 1177.5212
2469 589.7679 1177.5212
2470 589.7679 1177.5212
2471 589.7679 1177.5212
2472 589.7679 1177.5212
2473 589.7679 1177.5212
2474 589.7679 1177.5212
2475 589.7679 1177.5212
2479 589.7679 1177.5212
2480 589.7679 1177.5212
2481 589.7679 1177.5212
2482 589.7679 1177.5212
2483 589.7679 1177.5212
2485 589.7679 1177.5212
2486 589.7679 1177.5212
2488 589.7679 1177.5212
2489 589.7679 1177.5212
2490 589.7679 1177.5212
2491 589.7679 1177.5212
2492 589.7679 1177.5212
2493 589.7679 1177.5212
2494 589.7679 1177.5212
2495 589.7679 1177.5212
2496 589.7679 1177.5212
2497 589.7679 1177.5212
2498 589.7679 1177.5212
2499 589.7679 1177.5212
2500 589.7679 1177.5212
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