MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | PRT1377_JBEI_1 |
| MS data file | : | PRT1377_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 23 Jan 2026 at 21:28:41 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 7,307 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 848 |
454.7066 | 907.3986 | 907.3957 | 3.29 | 0 | 5 | 0.46 | 1Score > 14 indicates identity |
ELMEGNAK + Deamidated (NQ); Oxidation (M) |
| 3057 |
630.2864 | 1258.5582 | 1258.5789 | -16.4 | 0 | 5 | 0.46 | 1Score > 14 indicates identity |
DDGLILNDNGGR + Deamidated (NQ) |
| 2817 |
616.7802 | 1231.5458 | 1231.5680 | -18.0 | 0 | 5 | 0.59 | 1Score > 15 indicates identity |
TDAEQAQELAR + Deamidated (NQ) |
| 5163 |
805.8662 | 1609.7178 | 1609.7150 | 1.80 | 1 | 5 | 0.61 | 1Score > 15 indicates identity |
MKLLMPDNMSQER + 2 Deamidated (NQ); Oxidation (M) |
| 7050 |
967.9399 | 1933.8652 | 1933.8476 | 9.14 | 0 | 5 | 0.9 | 1Score > 17 indicates identity |
MSTQYETQGYTINNAGR + Deamidated (NQ) |
| 439 |
414.1881 | 826.3616 | 826.3677 | -7.33 | 0 | 5 | 0.39 | 1Score > 13 indicates identity |
MATVSMR + 2 Oxidation (M) |
| 2083 |
549.2496 | 1096.4846 | 1096.4753 | 8.49 | 1 | 5 | 0.58 | 1Score > 15 indicates identity |
GDARMMASSR + Oxidation (M) |
| 4422 |
751.8416 | 1501.6686 | 1501.6871 | -12.3 | 0 | 5 | 0.93 | 1Score > 17 indicates identity |
AMGVDYAAEYLQR + Oxidation (M) |
| 5255 |
819.3723 | 1636.7300 | 1636.7185 | 7.05 | 0 | 5 | 0.84 | 1Score > 16 indicates identity |
VGGLSVMCEDAEAAGR + Oxidation (M) |
| 4681 |
765.3478 | 1528.6810 | 1528.7005 | -12.7 | 0 | 5 | 0.55 | 1Score > 15 indicates identity |
DLSALEDAAEDQPR |
| 4187 |
724.8293 | 1447.6440 | 1447.6725 | -19.7 | 1 | 5 | 0.43 | 1Score > 13 indicates identity |
EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M) |
| 2389 |
576.2618 | 1150.5090 | 1150.5288 | -17.2 | 0 | 5 | 0.65 | 1Score > 15 indicates identity |
QTQLTEEMR + Oxidation (M) |
| 6919 |
954.4337 | 1906.8528 | 1906.8690 | -8.48 | 1 | 5 | 1 | 1Score > 17 indicates identity |
LSAEDNARVNVAMNQTR + 3 Deamidated (NQ); Oxidation (M) |
| 1103 |
468.2127 | 934.4108 | 934.4243 | -14.4 | 0 | 5 | 0.67 | 1Score > 15 indicates identity |
SETDGVEAK |
| 3668 |
684.3109 | 1366.6072 | 1366.6221 | -10.8 | 0 | 4 | 0.78 | 1Score > 16 indicates identity |
LANAAITDMQMR + Deamidated (NQ); 2 Oxidation (M) |
| 4814 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 4 | 0.67 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 5011 |
792.3600 | 1582.7054 | 1582.6987 | 4.26 | 0 | 4 | 0.59 | 1Score > 15 indicates identity |
WEVHNFTMGYQR + Oxidation (M) |
| 4100 |
724.8293 | 1447.6440 | 1447.6725 | -19.7 | 1 | 4 | 0.45 | 1Score > 13 indicates identity |
ECAAEITDKAGQR |
| 5401 |
832.8785 | 1663.7424 | 1663.7723 | -17.9 | 1 | 4 | 0.75 | 1Score > 16 indicates identity |
TNGLMAQAQQQLGKR + 5 Deamidated (NQ); Oxidation (M) |
| 3675 |
684.3109 | 1366.6072 | 1366.5863 | 15.3 | 0 | 4 | 0.8 | 1Score > 16 indicates identity |
NEPEWMLEFR + Deamidated (NQ); Oxidation (M) |
| 3803 |
697.8171 | 1393.6196 | 1393.6329 | -9.53 | 1 | 4 | 0.88 | 1Score > 16 indicates identity |
EEMATEMERLR |
| 1870 |
535.7434 | 1069.4722 | 1069.4862 | -13.1 | 0 | 4 | 0.37 | 1Score > 13 indicates identity |
YCVNSASLR + Deamidated (NQ) |
| 6622 |
927.4214 | 1852.8282 | 1852.8625 | -18.5 | 1 | 4 | 0.94 | 1Score > 17 indicates identity |
NQKIGSLGMDVYENER + Deamidated (NQ) |
| 6717 |
940.9276 | 1879.8406 | 1879.8258 | 7.90 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M) |
| 3452 |
670.8048 | 1339.5950 | 1339.6165 | -16.0 | 1 | 4 | 0.89 | 1Score > 16 indicates identity |
AMQWQMDFRK |
| 3870 |
697.8171 | 1393.6196 | 1393.6217 | -1.50 | 0 | 4 | 0.9 | 1Score > 16 indicates identity |
SMQDPIADMLTR + Deamidated (NQ); Oxidation (M) |
| 4847 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 4 | 0.71 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 6003 |
873.4014 | 1744.7882 | 1744.8171 | -16.5 | 1 | 4 | 1.4 | 1Score > 18 indicates identity |
HQKIIDMAMNGVGCR + Oxidation (M) |
| 4959 |
792.3600 | 1582.7054 | 1582.7223 | -10.6 | 0 | 4 | 0.62 | 1Score > 15 indicates identity |
QNHLGSVQEEGNLR + 3 Deamidated (NQ) |
| 4099 |
724.8293 | 1447.6440 | 1447.6725 | -19.7 | 1 | 4 | 0.47 | 1Score > 13 indicates identity |
ECAAEITDKAGQR |
| 4107 |
724.8293 | 1447.6440 | 1447.6725 | -19.7 | 1 | 4 | 0.47 | 1Score > 13 indicates identity |
ECAAEITDKAGQR |
| 4415 |
751.8416 | 1501.6686 | 1501.6943 | -17.1 | 1 | 4 | 1 | 1Score > 17 indicates identity |
EKMQTNPQNQQR + Deamidated (NQ) |
| 256 |
400.6820 | 799.3494 | 799.3573 | -9.78 | 0 | 4 | 0.38 | 1Score > 13 indicates identity |
HAGNSGTR + Deamidated (NQ) |
| 4667 |
765.3478 | 1528.6810 | 1528.7005 | -12.7 | 0 | 4 | 0.61 | 1Score > 15 indicates identity |
EVPGVQLTNEGDNR + 2 Deamidated (NQ) |
| 2193 |
562.7557 | 1123.4968 | 1123.4894 | 6.65 | 0 | 4 | 0.59 | 1Score > 14 indicates identity |
NLNDTNYNR + Deamidated (NQ) |
| 5834 |
859.8907 | 1717.7668 | 1717.7808 | -8.14 | 1 | 4 | 0.71 | 1Score > 15 indicates identity |
GHSFEFRINGEDPGR + Deamidated (NQ) |
| 6873 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 6736 |
940.9276 | 1879.8406 | 1879.8291 | 6.13 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
MIQEQTMLNVADNSGAR + 3 Deamidated (NQ) |
| 4406 |
751.8416 | 1501.6686 | 1501.6541 | 9.70 | 1 | 4 | 1 | 1Score > 17 indicates identity |
GRSMDSVMAQYQK + 2 Deamidated (NQ) |
| 6867 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 3811 |
697.8171 | 1393.6196 | 1393.6249 | -3.75 | 0 | 4 | 0.93 | 1Score > 16 indicates identity |
QVPVEEDAYNTK + 2 Deamidated (NQ) |
| 781 |
441.2004 | 880.3862 | 880.3927 | -7.31 | 0 | 4 | 0.63 | 1Score > 15 indicates identity |
DGDGFGVSK |
| 4771 |
778.8539 | 1555.6932 | 1555.7235 | -19.5 | 1 | 4 | 0.75 | 1Score > 15 indicates identity |
CAAGTGRFLDVMSR + Oxidation (M) |
| 2567 |
589.7679 | 1177.5212 | 1177.5107 | 8.95 | 0 | 4 | 0.57 | 1Score > 14 indicates identity |
MQQSIDPAMR + 2 Deamidated (NQ) |
| 2087 |
549.2496 | 1096.4846 | 1096.4971 | -11.4 | 1 | 4 | 0.67 | 1Score > 15 indicates identity |
MRAGGFEEGK + Oxidation (M) |
| 5323 |
819.3723 | 1636.7300 | 1636.7119 | 11.1 | 1 | 4 | 0.97 | 1Score > 16 indicates identity |
RMGELMAESHASMR + 2 Oxidation (M) |
| 5444 |
832.8785 | 1663.7424 | 1663.7157 | 16.1 | 0 | 4 | 0.83 | 1Score > 16 indicates identity |
VPPGMTMMYHAQER + Deamidated (NQ); Oxidation (M) |
| 4104 |
724.8293 | 1447.6440 | 1447.6249 | 13.2 | 0 | 4 | 0.51 | 1Score > 13 indicates identity |
ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M) |
| 3321 |
657.2986 | 1312.5826 | 1312.5870 | -3.33 | 0 | 4 | 0.9 | 1Score > 16 indicates identity |
SYITGEFGHMR + Oxidation (M) |
| 6336 |
913.8986 | 1825.7826 | 1825.8186 | -19.7 | 1 | 4 | 0.59 | 1Score > 14 indicates identity |
ERQTGMQMVQSVLER + 3 Deamidated (NQ); 2 Oxidation (M) |
| 5620 |
846.3846 | 1690.7546 | 1690.7403 | 8.49 | 0 | 4 | 0.86 | 1Score > 16 indicates identity |
SEMGDLAQSVSHMQR + Oxidation (M) |
| 4094 |
724.8293 | 1447.6440 | 1447.6725 | -19.7 | 1 | 4 | 0.52 | 1Score > 13 indicates identity |
ECAAEITDKAGQR |
| 7060 |
967.9399 | 1933.8652 | 1933.8476 | 9.14 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
MSTQYETQGYTINNAGR + Deamidated (NQ) |
| 152 |
387.1817 | 772.3488 | 772.3351 | 17.7 | 0 | 4 | 0.41 | 1Score > 13 indicates identity |
DPNGDVR + Deamidated (NQ) |
| 2587 |
589.7679 | 1177.5212 | 1177.5107 | 8.95 | 0 | 4 | 0.6 | 1Score > 14 indicates identity |
MQQSIDPAMR + 2 Deamidated (NQ) |
| 3815 |
697.8171 | 1393.6196 | 1393.6078 | 8.49 | 1 | 4 | 1 | 1Score > 16 indicates identity |
RNGMSGVQCVER + 2 Deamidated (NQ) |
| 1703 |
522.2372 | 1042.4598 | 1042.4614 | -1.50 | 0 | 4 | 0.42 | 1Score > 13 indicates identity |
QQMEGHASR |
| 4858 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 4 | 0.79 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
| 6561 |
927.4214 | 1852.8282 | 1852.8631 | -18.8 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
SSIQLISSNWPYWNR + 3 Deamidated (NQ) |
| 6525 |
927.4214 | 1852.8282 | 1852.8389 | -5.74 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
SLPWQAVWEMYCQR |
| 5663 |
846.3846 | 1690.7546 | 1690.7484 | 3.72 | 0 | 4 | 0.89 | 1Score > 16 indicates identity |
QYLMQFCPDAVFR + Deamidated (NQ); Oxidation (M) |
| 4423 |
751.8416 | 1501.6686 | 1501.6943 | -17.1 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
EKMQTNPQNQQR + Deamidated (NQ) |
| 6263 |
900.4092 | 1798.8038 | 1798.8308 | -15.0 | 0 | 4 | 0.74 | 1Score > 15 indicates identity |
IYDSDSILSMHHEPR |
| 1841 |
535.7434 | 1069.4722 | 1069.4862 | -13.1 | 1 | 4 | 0.43 | 1Score > 13 indicates identity |
EAMGFSEKR + Oxidation (M) |
| 3737 |
684.3109 | 1366.6072 | 1366.6266 | -14.1 | 1 | 4 | 0.94 | 1Score > 16 indicates identity |
DWRETNIDYR |
| 661 |
441.1924 | 880.3702 | 880.3562 | 15.9 | 0 | 4 | 0.56 | 1Score > 14 indicates identity |
EDEAFNR + Deamidated (NQ) |
| 1726 |
522.2372 | 1042.4598 | 1042.4614 | -1.50 | 0 | 4 | 0.43 | 1Score > 13 indicates identity |
QQMEGHASR |
| 6165 |
886.9030 | 1771.7914 | 1771.8265 | -19.8 | 0 | 4 | 0.96 | 1Score > 16 indicates identity |
TYDTNVEGSIVYGTPR + Deamidated (NQ) |
| 4675 |
765.3478 | 1528.6810 | 1528.6576 | 15.3 | 0 | 4 | 0.69 | 1Score > 15 indicates identity |
HQEMGQPNVSTER + Deamidated (NQ); Oxidation (M) |
| 2085 |
549.2496 | 1096.4846 | 1096.4753 | 8.49 | 1 | 4 | 0.73 | 1Score > 15 indicates identity |
GDARMMASSR + Oxidation (M) |
| 5853 |
873.3959 | 1744.7772 | 1744.7991 | -12.5 | 1 | 4 | 1.4 | 1Score > 18 indicates identity |
EYTWANLMERNYR |
| 7124 |
967.9399 | 1933.8652 | 1933.8840 | -9.68 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
MGWSGEPTTSELKNPER + Oxidation (M) |
| 4641 |
765.3478 | 1528.6810 | 1528.6576 | 15.3 | 0 | 4 | 0.7 | 1Score > 15 indicates identity |
HQEMGQPNVSTER + Deamidated (NQ); Oxidation (M) |
| 7021 |
967.9399 | 1933.8652 | 1933.8993 | -17.6 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
MSHVELQPGFDFQQAGK + Oxidation (M) |
| 1592 |
508.7311 | 1015.4476 | 1015.4570 | -9.24 | 0 | 4 | 0.51 | 1Score > 13 indicates identity |
TADEQQAPR + Deamidated (NQ) |
| 2857 |
616.7802 | 1231.5458 | 1231.5503 | -3.62 | 0 | 4 | 0.77 | 1Score > 15 indicates identity |
MTDLHTDVER + Oxidation (M) |
| 5079 |
805.8662 | 1609.7178 | 1609.7381 | -12.6 | 0 | 4 | 0.79 | 1Score > 15 indicates identity |
AFEMGWRPDMSGVK |
| 4112 |
724.8293 | 1447.6440 | 1447.6249 | 13.2 | 0 | 4 | 0.55 | 1Score > 13 indicates identity |
ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M) |
| 4309 |
738.3355 | 1474.6564 | 1474.6663 | -6.70 | 0 | 4 | 0.86 | 1Score > 15 indicates identity |
SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M) |
| 7110 |
967.9399 | 1933.8652 | 1933.8298 | 18.3 | 1 | 4 | 1.2 | 1Score > 17 indicates identity |
QSMGFDTNMEGGFLARR + 2 Deamidated (NQ); Oxidation (M) |
| 3087 |
630.2864 | 1258.5582 | 1258.5724 | -11.3 | 1 | 4 | 0.6 | 1Score > 14 indicates identity |
TAMGEPGPDGRR + Oxidation (M) |
| 4148 |
724.8293 | 1447.6440 | 1447.6476 | -2.42 | 0 | 4 | 0.56 | 1Score > 13 indicates identity |
FCQALMTELYR + Deamidated (NQ); Oxidation (M) |
| 3312 |
657.2986 | 1312.5826 | 1312.5757 | 5.26 | 0 | 3 | 0.99 | 1Score > 16 indicates identity |
EYTWANLMER + Deamidated (NQ) |
| 2020 |
549.2496 | 1096.4846 | 1096.4753 | 8.49 | 1 | 3 | 0.77 | 1Score > 15 indicates identity |
GDARMMASSR + Oxidation (M) |
| 5800 |
859.8907 | 1717.7668 | 1717.7907 | -13.9 | 1 | 3 | 0.84 | 1Score > 15 indicates identity |
SVVNNDPQGYPNRVR + 4 Deamidated (NQ) |
| 3515 |
670.8048 | 1339.5950 | 1339.6125 | -13.0 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
AANQAFMMERR + Oxidation (M) |
| 2975 |
630.2864 | 1258.5582 | 1258.5621 | -3.03 | 0 | 3 | 0.61 | 1Score > 14 indicates identity |
ISMMPGMPSHR + Oxidation (M) |
| 4189 |
724.8293 | 1447.6440 | 1447.6725 | -19.7 | 1 | 3 | 0.57 | 1Score > 13 indicates identity |
EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M) |
| 5927 |
873.3969 | 1744.7792 | 1744.8097 | -17.4 | 1 | 3 | 1.6 | 1Score > 18 indicates identity |
MSHIQRETSCSRPR + Deamidated (NQ) |
| 6875 |
954.4337 | 1906.8528 | 1906.8784 | -13.4 | 0 | 3 | 1.3 | 1Score > 17 indicates identity |
MSQFFYIHPDNPQQR |
| 3627 |
684.3109 | 1366.6072 | 1366.6299 | -16.6 | 1 | 3 | 1 | 1Score > 16 indicates identity |
GAAMEGQYKGAQR + Deamidated (NQ) |
| 6729 |
940.9276 | 1879.8406 | 1879.8305 | 5.41 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
QSHGNPLMKQGHQMQR + 4 Deamidated (NQ) |
| 2878 |
616.7802 | 1231.5458 | 1231.5503 | -3.62 | 0 | 3 | 0.81 | 1Score > 15 indicates identity |
MTDLHTDVER + Oxidation (M) |
| 2752 |
603.2741 | 1204.5336 | 1204.5394 | -4.75 | 1 | 3 | 1 | 1Score > 16 indicates identity |
EAAASEKDPCK |
| 257 |
400.6820 | 799.3494 | 799.3573 | -9.78 | 0 | 3 | 0.47 | 1Score > 13 indicates identity |
HAGNSGTR + Deamidated (NQ) |
| 4418 |
751.8416 | 1501.6686 | 1501.6943 | -17.1 | 1 | 3 | 1.3 | 1Score > 17 indicates identity |
EKMQTNPQNQQR + Deamidated (NQ) |
| 65 |
387.1779 | 772.3412 | 772.3351 | 7.90 | 0 | 3 | 0.47 | 1Score > 13 indicates identity |
DPNGDVR + Deamidated (NQ) |
| 3998 |
711.3232 | 1420.6318 | 1420.6187 | 9.25 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M) |
| 5565 |
846.3846 | 1690.7546 | 1690.7661 | -6.78 | 1 | 3 | 0.98 | 1Score > 16 indicates identity |
GYSDTQGMLGVKYSW |
| 4854 |
778.8539 | 1555.6932 | 1555.7163 | -14.8 | 0 | 3 | 0.89 | 1Score > 15 indicates identity |
DMFWQLMVDLSR + Oxidation (M) |
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