MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2272 562.7557 1123.4968
2273 562.7557 1123.4968
2274 562.7557 1123.4968
2276 562.7557 1123.4968
2277 562.7557 1123.4968
2278 562.7557 1123.4968
2279 562.7557 1123.4968
2280 562.7557 1123.4968
2281 562.7624 1123.5102
2282 562.7634 1123.5122
2284 576.2618 1150.5090
2285 576.2618 1150.5090
2286 576.2618 1150.5090
2287 576.2618 1150.5090
2288 576.2618 1150.5090
2289 576.2618 1150.5090
2290 576.2618 1150.5090
2291 576.2618 1150.5090
2292 576.2618 1150.5090
2293 576.2618 1150.5090
2294 576.2618 1150.5090
2295 576.2618 1150.5090
2296 576.2618 1150.5090
2297 576.2618 1150.5090
2298 576.2618 1150.5090
2299 576.2618 1150.5090
2300 576.2618 1150.5090
2301 576.2618 1150.5090
2302 576.2618 1150.5090
2303 576.2618 1150.5090
2304 576.2618 1150.5090
2305 576.2618 1150.5090
2307 576.2618 1150.5090
2309 576.2618 1150.5090
2311 576.2618 1150.5090
2312 576.2618 1150.5090
2313 576.2618 1150.5090
2315 576.2618 1150.5090
2316 576.2618 1150.5090
2319 576.2618 1150.5090
2320 576.2618 1150.5090
2321 576.2618 1150.5090
2322 576.2618 1150.5090
2324 576.2618 1150.5090
2325 576.2618 1150.5090
2327 576.2618 1150.5090
2330 576.2618 1150.5090
2331 576.2618 1150.5090
2332 576.2618 1150.5090
2333 576.2618 1150.5090
2334 576.2618 1150.5090
2335 576.2618 1150.5090
2336 576.2618 1150.5090
2337 576.2618 1150.5090
2338 576.2618 1150.5090
2339 576.2618 1150.5090
2340 576.2618 1150.5090
2341 576.2618 1150.5090
2342 576.2618 1150.5090
2343 576.2618 1150.5090
2344 576.2618 1150.5090
2345 576.2618 1150.5090
2346 576.2618 1150.5090
2347 576.2618 1150.5090
2348 576.2618 1150.5090
2349 576.2618 1150.5090
2350 576.2618 1150.5090
2351 576.2618 1150.5090
2352 576.2618 1150.5090
2353 576.2618 1150.5090
2354 576.2618 1150.5090
2355 576.2618 1150.5090
2356 576.2618 1150.5090
2357 576.2618 1150.5090
2358 576.2618 1150.5090
2361 576.2618 1150.5090
2363 576.2618 1150.5090
2364 576.2618 1150.5090
2365 576.2618 1150.5090
2366 576.2618 1150.5090
2367 576.2618 1150.5090
2368 576.2618 1150.5090
2370 576.2618 1150.5090
2371 576.2618 1150.5090
2372 576.2618 1150.5090
2373 576.2618 1150.5090
2374 576.2618 1150.5090
2375 576.2618 1150.5090
2376 576.2618 1150.5090
2377 576.2618 1150.5090
2378 576.2618 1150.5090
2379 576.2618 1150.5090
2380 576.2618 1150.5090
2381 576.2618 1150.5090
2382 576.2618 1150.5090
2383 576.2618 1150.5090
2385 576.2618 1150.5090
2386 576.2618 1150.5090
2387 576.2618 1150.5090
2388 576.2618 1150.5090
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