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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2159 562.7557 1123.4968
2160 562.7557 1123.4968
2161 562.7557 1123.4968
2162 562.7557 1123.4968
2163 562.7557 1123.4968
2164 562.7557 1123.4968
2166 562.7557 1123.4968
2167 562.7557 1123.4968
2168 562.7557 1123.4968
2169 562.7557 1123.4968
2170 562.7557 1123.4968
2171 562.7557 1123.4968
2173 562.7557 1123.4968
2174 562.7557 1123.4968
2175 562.7557 1123.4968
2176 562.7557 1123.4968
2177 562.7557 1123.4968
2179 562.7557 1123.4968
2180 562.7557 1123.4968
2181 562.7557 1123.4968
2182 562.7557 1123.4968
2183 562.7557 1123.4968
2185 562.7557 1123.4968
2186 562.7557 1123.4968
2187 562.7557 1123.4968
2188 562.7557 1123.4968
2189 562.7557 1123.4968
2191 562.7557 1123.4968
2192 562.7557 1123.4968
2194 562.7557 1123.4968
2195 562.7557 1123.4968
2196 562.7557 1123.4968
2199 562.7557 1123.4968
2200 562.7557 1123.4968
2201 562.7557 1123.4968
2202 562.7557 1123.4968
2203 562.7557 1123.4968
2204 562.7557 1123.4968
2205 562.7557 1123.4968
2206 562.7557 1123.4968
2207 562.7557 1123.4968
2208 562.7557 1123.4968
2209 562.7557 1123.4968
2210 562.7557 1123.4968
2211 562.7557 1123.4968
2212 562.7557 1123.4968
2213 562.7557 1123.4968
2214 562.7557 1123.4968
2215 562.7557 1123.4968
2216 562.7557 1123.4968
2217 562.7557 1123.4968
2218 562.7557 1123.4968
2219 562.7557 1123.4968
2220 562.7557 1123.4968
2221 562.7557 1123.4968
2222 562.7557 1123.4968
2223 562.7557 1123.4968
2224 562.7557 1123.4968
2225 562.7557 1123.4968
2226 562.7557 1123.4968
2227 562.7557 1123.4968
2228 562.7557 1123.4968
2229 562.7557 1123.4968
2230 562.7557 1123.4968
2231 562.7557 1123.4968
2233 562.7557 1123.4968
2234 562.7557 1123.4968
2235 562.7557 1123.4968
2236 562.7557 1123.4968
2237 562.7557 1123.4968
2238 562.7557 1123.4968
2239 562.7557 1123.4968
2240 562.7557 1123.4968
2241 562.7557 1123.4968
2242 562.7557 1123.4968
2243 562.7557 1123.4968
2244 562.7557 1123.4968
2245 562.7557 1123.4968
2248 562.7557 1123.4968
2249 562.7557 1123.4968
2250 562.7557 1123.4968
2251 562.7557 1123.4968
2252 562.7557 1123.4968
2253 562.7557 1123.4968
2254 562.7557 1123.4968
2255 562.7557 1123.4968
2256 562.7557 1123.4968
2257 562.7557 1123.4968
2259 562.7557 1123.4968
2260 562.7557 1123.4968
2261 562.7557 1123.4968
2262 562.7557 1123.4968
2263 562.7557 1123.4968
2264 562.7557 1123.4968
2265 562.7557 1123.4968
2266 562.7557 1123.4968
2267 562.7557 1123.4968
2268 562.7557 1123.4968
2269 562.7557 1123.4968
2271 562.7557 1123.4968
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