MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2017 549.2496 1096.4846
2019 549.2496 1096.4846
2022 549.2496 1096.4846
2023 549.2496 1096.4846
2033 549.2496 1096.4846
2034 549.2496 1096.4846
2038 549.2496 1096.4846
2039 549.2496 1096.4846
2040 549.2496 1096.4846
2044 549.2496 1096.4846
2049 549.2496 1096.4846
2051 549.2496 1096.4846
2055 549.2496 1096.4846
2056 549.2496 1096.4846
2058 549.2496 1096.4846
2059 549.2496 1096.4846
2061 549.2496 1096.4846
2062 549.2496 1096.4846
2063 549.2496 1096.4846
2064 549.2496 1096.4846
2065 549.2496 1096.4846
2066 549.2496 1096.4846
2067 549.2496 1096.4846
2068 549.2496 1096.4846
2069 549.2496 1096.4846
2070 549.2496 1096.4846
2072 549.2496 1096.4846
2073 549.2496 1096.4846
2074 549.2496 1096.4846
2075 549.2496 1096.4846
2076 549.2496 1096.4846
2077 549.2496 1096.4846
2078 549.2496 1096.4846
2079 549.2496 1096.4846
2080 549.2496 1096.4846
2081 549.2496 1096.4846
2082 549.2496 1096.4846
2084 549.2496 1096.4846
2086 549.2496 1096.4846
2088 549.2496 1096.4846
2089 549.2496 1096.4846
2090 549.2496 1096.4846
2091 549.2496 1096.4846
2093 549.2496 1096.4846
2094 549.2496 1096.4846
2095 549.2496 1096.4846
2097 549.2496 1096.4846
2098 549.2496 1096.4846
2099 549.2496 1096.4846
2101 549.2496 1096.4846
2103 549.2496 1096.4846
2104 549.2496 1096.4846
2105 549.2496 1096.4846
2106 549.2496 1096.4846
2107 549.2496 1096.4846
2108 549.2496 1096.4846
2109 549.2496 1096.4846
2111 549.2496 1096.4846
2112 549.2496 1096.4846
2113 549.2496 1096.4846
2114 549.2496 1096.4846
2115 549.2496 1096.4846
2116 549.2496 1096.4846
2117 549.2496 1096.4846
2119 549.2519 1096.4892
2120 562.7499 1123.4852
2121 562.7532 1123.4918
2122 562.7557 1123.4968
2123 562.7557 1123.4968
2124 562.7557 1123.4968
2125 562.7557 1123.4968
2126 562.7557 1123.4968
2127 562.7557 1123.4968
2128 562.7557 1123.4968
2129 562.7557 1123.4968
2130 562.7557 1123.4968
2131 562.7557 1123.4968
2132 562.7557 1123.4968
2133 562.7557 1123.4968
2134 562.7557 1123.4968
2135 562.7557 1123.4968
2136 562.7557 1123.4968
2137 562.7557 1123.4968
2138 562.7557 1123.4968
2139 562.7557 1123.4968
2140 562.7557 1123.4968
2141 562.7557 1123.4968
2142 562.7557 1123.4968
2143 562.7557 1123.4968
2144 562.7557 1123.4968
2146 562.7557 1123.4968
2147 562.7557 1123.4968
2149 562.7557 1123.4968
2152 562.7557 1123.4968
2153 562.7557 1123.4968
2154 562.7557 1123.4968
2155 562.7557 1123.4968
2156 562.7557 1123.4968
2157 562.7557 1123.4968
2158 562.7557 1123.4968
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