MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1901 535.7434 1069.4722
1902 535.7434 1069.4722
1903 535.7434 1069.4722
1904 535.7434 1069.4722
1905 535.7434 1069.4722
1907 535.7434 1069.4722
1908 535.7434 1069.4722
1909 535.7434 1069.4722
1910 535.7434 1069.4722
1911 535.7434 1069.4722
1912 535.7434 1069.4722
1913 535.7434 1069.4722
1914 535.7434 1069.4722
1915 535.7434 1069.4722
1916 535.7434 1069.4722
1917 535.7434 1069.4722
1919 535.7434 1069.4722
1920 535.7434 1069.4722
1921 535.7434 1069.4722
1922 535.7434 1069.4722
1923 535.7434 1069.4722
1924 535.7434 1069.4722
1925 535.7434 1069.4722
1926 535.7434 1069.4722
1927 535.7434 1069.4722
1928 535.7434 1069.4722
1929 535.7434 1069.4722
1930 535.7434 1069.4722
1931 535.7434 1069.4722
1932 535.7434 1069.4722
1933 535.7434 1069.4722
1935 535.7434 1069.4722
1936 535.7434 1069.4722
1937 535.7434 1069.4722
1938 535.7434 1069.4722
1940 535.7434 1069.4722
1941 535.7434 1069.4722
1942 535.7434 1069.4722
1943 535.7434 1069.4722
1944 535.7434 1069.4722
1945 535.7434 1069.4722
1946 535.7434 1069.4722
1947 535.7434 1069.4722
1948 535.7434 1069.4722
1949 535.7434 1069.4722
1950 535.7434 1069.4722
1951 535.7434 1069.4722
1952 535.7434 1069.4722
1953 535.7434 1069.4722
1954 535.7434 1069.4722
1955 535.7434 1069.4722
1956 535.7514 1069.4882
1957 549.2390 1096.4634
1958 549.2496 1096.4846
1959 549.2496 1096.4846
1960 549.2496 1096.4846
1961 549.2496 1096.4846
1962 549.2496 1096.4846
1964 549.2496 1096.4846
1965 549.2496 1096.4846
1967 549.2496 1096.4846
1969 549.2496 1096.4846
1972 549.2496 1096.4846
1975 549.2496 1096.4846
1977 549.2496 1096.4846
1978 549.2496 1096.4846
1979 549.2496 1096.4846
1980 549.2496 1096.4846
1982 549.2496 1096.4846
1983 549.2496 1096.4846
1984 549.2496 1096.4846
1985 549.2496 1096.4846
1986 549.2496 1096.4846
1988 549.2496 1096.4846
1989 549.2496 1096.4846
1990 549.2496 1096.4846
1991 549.2496 1096.4846
1992 549.2496 1096.4846
1993 549.2496 1096.4846
1994 549.2496 1096.4846
1996 549.2496 1096.4846
1997 549.2496 1096.4846
1998 549.2496 1096.4846
1999 549.2496 1096.4846
2000 549.2496 1096.4846
2001 549.2496 1096.4846
2002 549.2496 1096.4846
2003 549.2496 1096.4846
2004 549.2496 1096.4846
2005 549.2496 1096.4846
2006 549.2496 1096.4846
2007 549.2496 1096.4846
2008 549.2496 1096.4846
2009 549.2496 1096.4846
2010 549.2496 1096.4846
2011 549.2496 1096.4846
2012 549.2496 1096.4846
2013 549.2496 1096.4846
2014 549.2496 1096.4846
2015 549.2496 1096.4846
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