MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1777 522.2414 1042.4682
1779 522.2417 1042.4688
1780 522.2418 1042.4690
1781 522.2420 1042.4694
1782 522.2421 1042.4696
1783 522.2423 1042.4700
1784 522.2425 1042.4704
1785 522.2427 1042.4708
1787 522.2430 1042.4714
1788 522.2430 1042.4714
1789 522.2436 1042.4726
1790 522.2438 1042.4730
1791 522.2443 1042.4740
1792 522.2445 1042.4744
1793 522.2446 1042.4746
1795 535.7434 1069.4722
1796 535.7434 1069.4722
1797 535.7434 1069.4722
1798 535.7434 1069.4722
1799 535.7434 1069.4722
1800 535.7434 1069.4722
1801 535.7434 1069.4722
1802 535.7434 1069.4722
1803 535.7434 1069.4722
1804 535.7434 1069.4722
1805 535.7434 1069.4722
1806 535.7434 1069.4722
1807 535.7434 1069.4722
1808 535.7434 1069.4722
1809 535.7434 1069.4722
1810 535.7434 1069.4722
1811 535.7434 1069.4722
1812 535.7434 1069.4722
1813 535.7434 1069.4722
1814 535.7434 1069.4722
1815 535.7434 1069.4722
1817 535.7434 1069.4722
1819 535.7434 1069.4722
1820 535.7434 1069.4722
1824 535.7434 1069.4722
1825 535.7434 1069.4722
1826 535.7434 1069.4722
1827 535.7434 1069.4722
1829 535.7434 1069.4722
1831 535.7434 1069.4722
1833 535.7434 1069.4722
1834 535.7434 1069.4722
1835 535.7434 1069.4722
1836 535.7434 1069.4722
1838 535.7434 1069.4722
1839 535.7434 1069.4722
1842 535.7434 1069.4722
1844 535.7434 1069.4722
1847 535.7434 1069.4722
1848 535.7434 1069.4722
1849 535.7434 1069.4722
1851 535.7434 1069.4722
1853 535.7434 1069.4722
1854 535.7434 1069.4722
1855 535.7434 1069.4722
1857 535.7434 1069.4722
1858 535.7434 1069.4722
1859 535.7434 1069.4722
1861 535.7434 1069.4722
1862 535.7434 1069.4722
1863 535.7434 1069.4722
1864 535.7434 1069.4722
1865 535.7434 1069.4722
1866 535.7434 1069.4722
1867 535.7434 1069.4722
1868 535.7434 1069.4722
1869 535.7434 1069.4722
1871 535.7434 1069.4722
1872 535.7434 1069.4722
1873 535.7434 1069.4722
1874 535.7434 1069.4722
1876 535.7434 1069.4722
1877 535.7434 1069.4722
1878 535.7434 1069.4722
1879 535.7434 1069.4722
1880 535.7434 1069.4722
1881 535.7434 1069.4722
1882 535.7434 1069.4722
1883 535.7434 1069.4722
1884 535.7434 1069.4722
1885 535.7434 1069.4722
1886 535.7434 1069.4722
1887 535.7434 1069.4722
1888 535.7434 1069.4722
1889 535.7434 1069.4722
1890 535.7434 1069.4722
1891 535.7434 1069.4722
1892 535.7434 1069.4722
1893 535.7434 1069.4722
1894 535.7434 1069.4722
1895 535.7434 1069.4722
1897 535.7434 1069.4722
1898 535.7434 1069.4722
1899 535.7434 1069.4722
1900 535.7434 1069.4722
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