MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1560 508.7311 1015.4476
1561 508.7311 1015.4476
1562 508.7311 1015.4476
1563 508.7311 1015.4476
1564 508.7311 1015.4476
1565 508.7311 1015.4476
1566 508.7311 1015.4476
1567 508.7311 1015.4476
1568 508.7311 1015.4476
1569 508.7311 1015.4476
1570 508.7311 1015.4476
1571 508.7311 1015.4476
1572 508.7311 1015.4476
1573 508.7311 1015.4476
1574 508.7311 1015.4476
1575 508.7311 1015.4476
1576 508.7311 1015.4476
1577 508.7311 1015.4476
1578 508.7311 1015.4476
1579 508.7311 1015.4476
1580 508.7311 1015.4476
1581 508.7311 1015.4476
1582 508.7311 1015.4476
1583 508.7311 1015.4476
1584 508.7311 1015.4476
1585 508.7311 1015.4476
1586 508.7311 1015.4476
1587 508.7311 1015.4476
1588 508.7311 1015.4476
1589 508.7311 1015.4476
1590 508.7311 1015.4476
1591 508.7311 1015.4476
1593 508.7311 1015.4476
1594 508.7311 1015.4476
1595 508.7311 1015.4476
1596 508.7311 1015.4476
1597 508.7311 1015.4476
1598 508.7311 1015.4476
1599 508.7311 1015.4476
1600 508.7311 1015.4476
1601 508.7311 1015.4476
1602 508.7311 1015.4476
1603 508.7311 1015.4476
1604 508.7311 1015.4476
1605 508.7311 1015.4476
1606 508.7311 1015.4476
1607 508.7311 1015.4476
1608 508.7311 1015.4476
1609 508.7311 1015.4476
1610 508.7311 1015.4476
1611 508.7311 1015.4476
1612 508.7311 1015.4476
1613 508.7311 1015.4476
1614 508.7311 1015.4476
1615 508.7311 1015.4476
1616 508.7311 1015.4476
1617 508.7311 1015.4476
1618 508.7311 1015.4476
1619 508.7311 1015.4476
1620 508.7311 1015.4476
1621 508.7311 1015.4476
1622 508.7311 1015.4476
1623 508.7311 1015.4476
1624 508.7311 1015.4476
1625 508.7311 1015.4476
1626 508.7361 1015.4576
1627 508.7371 1015.4596
1628 508.7378 1015.4610
1629 508.7378 1015.4610
1630 508.7409 1015.4672
1631 522.2269 1042.4392
1634 522.2319 1042.4492
1635 522.2331 1042.4516
1636 522.2355 1042.4564
1637 522.2357 1042.4568
1639 522.2372 1042.4598
1640 522.2372 1042.4598
1641 522.2372 1042.4598
1642 522.2372 1042.4598
1643 522.2372 1042.4598
1644 522.2372 1042.4598
1645 522.2372 1042.4598
1646 522.2372 1042.4598
1647 522.2372 1042.4598
1648 522.2372 1042.4598
1649 522.2372 1042.4598
1650 522.2372 1042.4598
1651 522.2372 1042.4598
1652 522.2372 1042.4598
1653 522.2372 1042.4598
1654 522.2372 1042.4598
1655 522.2372 1042.4598
1656 522.2372 1042.4598
1657 522.2372 1042.4598
1658 522.2372 1042.4598
1659 522.2372 1042.4598
1660 522.2372 1042.4598
1661 522.2372 1042.4598
1662 522.2372 1042.4598
1663 522.2372 1042.4598
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