MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1459 495.2250 988.4354
1460 495.2250 988.4354
1461 495.2250 988.4354
1462 495.2250 988.4354
1463 495.2253 988.4360
1464 495.2254 988.4362
1465 495.2255 988.4364
1466 495.2262 988.4378
1467 495.2279 988.4412
1468 508.7262 1015.4378
1469 508.7267 1015.4388
1470 508.7271 1015.4396
1471 508.7271 1015.4396
1472 508.7278 1015.4410
1473 508.7279 1015.4412
1474 508.7290 1015.4434
1475 508.7295 1015.4444
1476 508.7297 1015.4448
1477 508.7298 1015.4450
1478 508.7311 1015.4476
1479 508.7311 1015.4476
1480 508.7311 1015.4476
1481 508.7311 1015.4476
1482 508.7311 1015.4476
1483 508.7311 1015.4476
1484 508.7311 1015.4476
1485 508.7311 1015.4476
1486 508.7311 1015.4476
1487 508.7311 1015.4476
1488 508.7311 1015.4476
1489 508.7311 1015.4476
1490 508.7311 1015.4476
1491 508.7311 1015.4476
1492 508.7311 1015.4476
1493 508.7311 1015.4476
1494 508.7311 1015.4476
1495 508.7311 1015.4476
1496 508.7311 1015.4476
1497 508.7311 1015.4476
1498 508.7311 1015.4476
1499 508.7311 1015.4476
1500 508.7311 1015.4476
1501 508.7311 1015.4476
1502 508.7311 1015.4476
1503 508.7311 1015.4476
1504 508.7311 1015.4476
1505 508.7311 1015.4476
1506 508.7311 1015.4476
1507 508.7311 1015.4476
1508 508.7311 1015.4476
1509 508.7311 1015.4476
1510 508.7311 1015.4476
1511 508.7311 1015.4476
1512 508.7311 1015.4476
1513 508.7311 1015.4476
1514 508.7311 1015.4476
1515 508.7311 1015.4476
1516 508.7311 1015.4476
1517 508.7311 1015.4476
1518 508.7311 1015.4476
1519 508.7311 1015.4476
1521 508.7311 1015.4476
1522 508.7311 1015.4476
1523 508.7311 1015.4476
1524 508.7311 1015.4476
1525 508.7311 1015.4476
1526 508.7311 1015.4476
1527 508.7311 1015.4476
1528 508.7311 1015.4476
1529 508.7311 1015.4476
1530 508.7311 1015.4476
1531 508.7311 1015.4476
1532 508.7311 1015.4476
1533 508.7311 1015.4476
1534 508.7311 1015.4476
1535 508.7311 1015.4476
1536 508.7311 1015.4476
1537 508.7311 1015.4476
1538 508.7311 1015.4476
1539 508.7311 1015.4476
1540 508.7311 1015.4476
1541 508.7311 1015.4476
1542 508.7311 1015.4476
1543 508.7311 1015.4476
1544 508.7311 1015.4476
1545 508.7311 1015.4476
1546 508.7311 1015.4476
1547 508.7311 1015.4476
1548 508.7311 1015.4476
1549 508.7311 1015.4476
1550 508.7311 1015.4476
1551 508.7311 1015.4476
1552 508.7311 1015.4476
1553 508.7311 1015.4476
1554 508.7311 1015.4476
1555 508.7311 1015.4476
1556 508.7311 1015.4476
1557 508.7311 1015.4476
1558 508.7311 1015.4476
1559 508.7311 1015.4476
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