MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1356 495.2227 988.4308
1357 495.2227 988.4308
1358 495.2228 988.4310
1359 495.2228 988.4310
1360 495.2228 988.4310
1361 495.2228 988.4310
1362 495.2228 988.4310
1363 495.2230 988.4314
1364 495.2231 988.4316
1365 495.2231 988.4316
1366 495.2231 988.4316
1367 495.2231 988.4316
1368 495.2231 988.4316
1369 495.2231 988.4316
1370 495.2232 988.4318
1371 495.2232 988.4318
1372 495.2232 988.4318
1373 495.2233 988.4320
1374 495.2233 988.4320
1375 495.2233 988.4320
1376 495.2233 988.4320
1377 495.2235 988.4324
1378 495.2236 988.4326
1379 495.2236 988.4326
1380 495.2236 988.4326
1381 495.2237 988.4328
1382 495.2237 988.4328
1383 495.2237 988.4328
1384 495.2238 988.4330
1385 495.2238 988.4330
1386 495.2238 988.4330
1387 495.2239 988.4332
1388 495.2239 988.4332
1389 495.2239 988.4332
1390 495.2240 988.4334
1391 495.2240 988.4334
1392 495.2240 988.4334
1393 495.2240 988.4334
1394 495.2241 988.4336
1395 495.2242 988.4338
1396 495.2242 988.4338
1397 495.2242 988.4338
1398 495.2242 988.4338
1399 495.2242 988.4338
1400 495.2242 988.4338
1401 495.2243 988.4340
1402 495.2243 988.4340
1403 495.2243 988.4340
1405 495.2245 988.4344
1406 495.2245 988.4344
1407 495.2246 988.4346
1408 495.2246 988.4346
1409 495.2246 988.4346
1410 495.2247 988.4348
1411 495.2248 988.4350
1412 495.2249 988.4352
1413 495.2250 988.4354
1414 495.2250 988.4354
1415 495.2250 988.4354
1416 495.2250 988.4354
1418 495.2250 988.4354
1419 495.2250 988.4354
1420 495.2250 988.4354
1421 495.2250 988.4354
1422 495.2250 988.4354
1423 495.2250 988.4354
1424 495.2250 988.4354
1425 495.2250 988.4354
1426 495.2250 988.4354
1427 495.2250 988.4354
1428 495.2250 988.4354
1429 495.2250 988.4354
1430 495.2250 988.4354
1431 495.2250 988.4354
1432 495.2250 988.4354
1433 495.2250 988.4354
1434 495.2250 988.4354
1435 495.2250 988.4354
1436 495.2250 988.4354
1437 495.2250 988.4354
1438 495.2250 988.4354
1439 495.2250 988.4354
1440 495.2250 988.4354
1441 495.2250 988.4354
1442 495.2250 988.4354
1443 495.2250 988.4354
1444 495.2250 988.4354
1445 495.2250 988.4354
1446 495.2250 988.4354
1447 495.2250 988.4354
1448 495.2250 988.4354
1449 495.2250 988.4354
1450 495.2250 988.4354
1451 495.2250 988.4354
1452 495.2250 988.4354
1453 495.2250 988.4354
1454 495.2250 988.4354
1455 495.2250 988.4354
1456 495.2250 988.4354
1458 495.2250 988.4354
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