MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1244 481.7188 961.4230
1245 481.7188 961.4230
1247 481.7188 961.4230
1248 481.7188 961.4230
1249 481.7188 961.4230
1250 481.7188 961.4230
1251 481.7188 961.4230
1253 481.7188 961.4230
1254 481.7188 961.4230
1255 481.7188 961.4230
1256 481.7188 961.4230
1257 481.7188 961.4230
1258 481.7188 961.4230
1259 481.7188 961.4230
1261 481.7188 961.4230
1262 481.7188 961.4230
1263 481.7188 961.4230
1264 481.7188 961.4230
1265 481.7188 961.4230
1266 481.7188 961.4230
1267 481.7188 961.4230
1268 481.7188 961.4230
1269 481.7188 961.4230
1271 481.7188 961.4230
1273 481.7188 961.4230
1274 481.7188 961.4230
1276 481.7188 961.4230
1277 481.7188 961.4230
1278 481.7188 961.4230
1280 481.7188 961.4230
1281 481.7188 961.4230
1282 481.7188 961.4230
1284 481.7188 961.4230
1285 481.7188 961.4230
1286 481.7188 961.4230
1287 481.7188 961.4230
1288 481.7188 961.4230
1289 481.7188 961.4230
1290 481.7188 961.4230
1291 481.7188 961.4230
1293 481.7188 961.4230
1294 481.7188 961.4230
1295 481.7188 961.4230
1296 481.7188 961.4230
1299 481.7188 961.4230
1300 481.7188 961.4230
1301 481.7267 961.4388
1302 481.7277 961.4408
1303 481.7281 961.4416
1304 481.7282 961.4418
1305 495.2165 988.4184
1307 495.2188 988.4230
1308 495.2199 988.4252
1309 495.2200 988.4254
1310 495.2201 988.4256
1311 495.2202 988.4258
1312 495.2202 988.4258
1313 495.2202 988.4258
1314 495.2204 988.4262
1315 495.2206 988.4266
1316 495.2208 988.4270
1317 495.2208 988.4270
1318 495.2208 988.4270
1319 495.2209 988.4272
1320 495.2209 988.4272
1321 495.2209 988.4272
1322 495.2210 988.4274
1323 495.2210 988.4274
1324 495.2212 988.4278
1325 495.2213 988.4280
1326 495.2215 988.4284
1327 495.2215 988.4284
1328 495.2216 988.4286
1329 495.2216 988.4286
1330 495.2218 988.4290
1331 495.2219 988.4292
1332 495.2219 988.4292
1333 495.2220 988.4294
1334 495.2220 988.4294
1335 495.2220 988.4294
1336 495.2221 988.4296
1337 495.2222 988.4298
1338 495.2222 988.4298
1339 495.2222 988.4298
1340 495.2222 988.4298
1341 495.2222 988.4298
1342 495.2222 988.4298
1343 495.2222 988.4298
1344 495.2223 988.4300
1345 495.2223 988.4300
1346 495.2224 988.4302
1347 495.2225 988.4304
1348 495.2225 988.4304
1349 495.2225 988.4304
1350 495.2225 988.4304
1351 495.2225 988.4304
1352 495.2226 988.4306
1353 495.2226 988.4306
1354 495.2227 988.4308
1355 495.2227 988.4308
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