MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1045 468.2127 934.4108 934.4178 -7.46 1 7 0.39 +1Score > 15 indicates identity LDEDCKR
414 414.1881 826.3616 826.3677 -7.33 0 7 0.24 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1024 468.2127 934.4108 934.4178 -7.46 1 7 0.39 +1Score > 15 indicates identity LDEDCKR
3799 697.8171 1393.6196 1393.6330 -9.56 0 7 0.49 +1Score > 16 indicates identity QLLEQMQADMR + 2 Oxidation (M)
1981 549.2496 1096.4846 1096.4978 -12.0 0 7 0.35 +1Score > 15 indicates identity YYGYTGAFR
2328 576.2618 1150.5090 1150.4891 17.4 0 7 0.39 +1Score > 15 indicates identity DEAQEQQFR + Deamidated (NQ)
377 414.1854 826.3562 826.3677 -13.9 0 7 0.24 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1896 535.7434 1069.4722 1069.4716 0.57 0 7 0.21 +1Score > 13 indicates identity DAGFQAFADK + Deamidated (NQ)
984 468.2127 934.4108 934.4178 -7.46 1 7 0.41 +1Score > 15 indicates identity LDEDCKR
6087 886.9030 1771.7914 1771.7584 18.7 1 7 0.5 +1Score > 16 indicates identity DPASDQMQHWKEQR + Deamidated (NQ); Oxidation (M)
4102 724.8293 1447.6440 1447.6725 -19.7 1 6 0.28 +1Score > 13 indicates identity ECAAEITDKAGQR
2197 562.7557 1123.4968 1123.4824 12.9 1 6 0.35 +1Score > 14 indicates identity RQMMGMEPK + Deamidated (NQ); Oxidation (M)
3383 657.2986 1312.5826 1312.5686 10.7 1 6 0.5 +1Score > 16 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
5624 846.3846 1690.7546 1690.7686 -8.24 0 6 0.48 +1Score > 16 indicates identity IDLTEWETNPESTR + Deamidated (NQ)
1457 495.2250 988.4354 988.4219 13.7 0 6 0.23 +1Score > 13 indicates identity GNHEVMMR + Oxidation (M)
5210 819.3723 1636.7300 1636.7403 -6.24 0 6 0.56 +1Score > 16 indicates identity QSEECYEVPIQQK
6196 900.4092 1798.8038 1798.7953 4.76 0 6 0.4 +1Score > 15 indicates identity CLNHMFAAEIYMQR + Oxidation (M)
1039 468.2127 934.4108 934.4178 -7.46 1 6 0.44 +1Score > 15 indicates identity LDEDCKR
4380 738.3355 1474.6564 1474.6728 -11.1 0 6 0.45 +1Score > 15 indicates identity NQYYGITAGPAYR + 2 Deamidated (NQ)
686 441.1935 880.3724 880.3562 18.4 0 6 0.29 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
4121 724.8293 1447.6440 1447.6249 13.2 0 6 0.3 +1Score > 13 indicates identity ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M)
4375 738.3355 1474.6564 1474.6776 -14.3 1 6 0.46 +1Score > 15 indicates identity NAWWVGSKDCPR
4108 724.8293 1447.6440 1447.6249 13.2 0 6 0.3 +1Score > 13 indicates identity ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M)
5947 873.3969 1744.7792 1744.7735 3.28 0 6 0.84 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
4764 778.8539 1555.6932 1555.7235 -19.5 1 6 0.46 +1Score > 15 indicates identity CAAGTGRFLDVMSR + Oxidation (M)
1822 535.7434 1069.4722 1069.4862 -13.1 0 6 0.24 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
4095 724.8293 1447.6440 1447.6725 -19.7 1 6 0.3 +1Score > 13 indicates identity ECAAEITDKAGQR
1875 535.7434 1069.4722 1069.4862 -13.1 0 6 0.24 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
5310 819.3723 1636.7300 1636.7403 -6.24 0 6 0.6 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
3652 684.3109 1366.6072 1366.6252 -13.1 0 6 0.54 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
2071 549.2496 1096.4846 1096.4978 -12.0 0 6 0.42 +1Score > 15 indicates identity YYGYTGAFR
743 441.2004 880.3862 880.3709 17.5 0 6 0.39 +1Score > 15 indicates identity ATDSATCR
2400 576.2618 1150.5090 1150.5254 -14.2 1 6 0.46 +1Score > 15 indicates identity LEFNNDNRK + 2 Deamidated (NQ)
6761 940.9276 1879.8406 1879.8258 7.90 0 6 0.74 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
6899 954.4337 1906.8528 1906.8818 -15.2 0 6 0.72 +1Score > 17 indicates identity GVAMVFPGQGAQWQGMAR + Deamidated (NQ); Oxidation (M)
4169 724.8293 1447.6440 1447.6725 -19.7 1 6 0.31 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
6699 940.9276 1879.8406 1879.8305 5.41 1 6 0.75 +1Score > 17 indicates identity QSHGNPLMKQGHQMQR + 4 Deamidated (NQ)
922 454.7066 907.3986 907.3817 18.6 1 6 0.34 +1Score > 14 indicates identity ENMGRER + Deamidated (NQ); Oxidation (M)
6528 927.4214 1852.8282 1852.8326 -2.36 1 6 0.64 +1Score > 17 indicates identity TLNSNQFDNEDAIKDK + 2 Deamidated (NQ)
589 427.6943 853.3740 853.3851 -13.0 0 6 0.25 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
5608 846.3846 1690.7546 1690.7872 -19.2 0 6 0.54 +1Score > 16 indicates identity HEDMYTAINELINK + Deamidated (NQ)
1032 468.2127 934.4108 934.4178 -7.46 1 6 0.49 +1Score > 15 indicates identity LDEDCKR
4090 724.8293 1447.6440 1447.6725 -19.7 1 6 0.32 +1Score > 13 indicates identity ECAAEITDKAGQR
3880 697.8171 1393.6196 1393.6217 -1.50 0 6 0.63 +1Score > 16 indicates identity SMQDPIADMLTR + Deamidated (NQ); Oxidation (M)
2258 562.7557 1123.4968 1123.4894 6.65 0 6 0.4 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
5528 846.3815 1690.7484 1690.7475 0.58 0 6 0.44 +1Score > 15 indicates identity ESSVPFSYYDNQQK
6212 900.4092 1798.8038 1798.7953 4.76 0 6 0.46 +1Score > 15 indicates identity CLNHMFAAEIYMQR + Oxidation (M)
1987 549.2496 1096.4846 1096.4971 -11.4 1 6 0.45 +1Score > 15 indicates identity MRAGGFEEGK + Oxidation (M)
3794 697.8171 1393.6196 1393.6329 -9.53 1 6 0.63 +1Score > 16 indicates identity EEMATEMERLR
4476 751.8416 1501.6686 1501.6772 -5.69 0 6 0.71 +1Score > 17 indicates identity YHQWMNELPER
704 441.1941 880.3736 880.3562 19.8 0 6 0.33 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
988 468.2127 934.4108 934.4178 -7.46 1 6 0.51 +1Score > 15 indicates identity LDEDCKR
437 414.1881 826.3616 826.3677 -7.33 0 6 0.31 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
4168 724.8293 1447.6440 1447.6249 13.2 0 6 0.34 +1Score > 13 indicates identity ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M)
4417 751.8416 1501.6686 1501.6943 -17.1 1 6 0.73 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
2359 576.2618 1150.5090 1150.4891 17.4 0 6 0.51 +1Score > 15 indicates identity DEAQEQQFR + Deamidated (NQ)
4266 738.3355 1474.6564 1474.6398 11.3 0 6 0.54 +1Score > 15 indicates identity YLYANMEVNGQR + 2 Deamidated (NQ); Oxidation (M)
5225 819.3723 1636.7300 1636.7185 7.05 0 6 0.68 +1Score > 16 indicates identity VGGLSVMCEDAEAAGR + Oxidation (M)
5390 832.8785 1663.7424 1663.7723 -17.9 1 6 0.59 +1Score > 16 indicates identity TNGLMAQAQQQLGKR + 5 Deamidated (NQ); Oxidation (M)
2819 616.7802 1231.5458 1231.5503 -3.62 0 5 0.49 +1Score > 15 indicates identity MTDLHTDVER + Oxidation (M)
463 414.1881 826.3616 826.3565 6.28 0 5 0.33 +1Score > 13 indicates identity MLGQMSK + Deamidated (NQ); 2 Oxidation (M)
5607 846.3846 1690.7546 1690.7403 8.49 0 5 0.59 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
562 427.6943 853.3740 853.3600 16.5 0 5 0.29 +1Score > 13 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
5869 873.3969 1744.7792 1744.8057 -15.1 1 5 1 +1Score > 18 indicates identity QGDFKSPEGFYSVQR + Deamidated (NQ)
5957 873.3969 1744.7792 1744.7800 -0.44 0 5 1 +1Score > 18 indicates identity GLMFTQPNQAAGYMAK + 2 Deamidated (NQ); Oxidation (M)
1707 522.2372 1042.4598 1042.4614 -1.50 0 5 0.29 +1Score > 13 indicates identity QQMEGHASR
6438 913.9153 1825.8160 1825.7900 14.2 0 5 0.81 +1Score > 17 indicates identity NGYNSAALNGDMNQALR + 2 Deamidated (NQ); Oxidation (M)
6520 927.4214 1852.8282 1852.8389 -5.74 0 5 0.74 +1Score > 17 indicates identity SLPWQAVWEMYCQR
1970 549.2496 1096.4846 1096.4825 1.93 0 5 0.49 +1Score > 15 indicates identity YVTWEDQR + Deamidated (NQ)
6852 954.4337 1906.8528 1906.8784 -13.4 0 5 0.84 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
5254 819.3723 1636.7300 1636.7185 7.05 0 5 0.71 +1Score > 16 indicates identity VGGLSVMCEDAEAAGR + Oxidation (M)
6861 954.4337 1906.8528 1906.8784 -13.4 0 5 0.85 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
1127 468.2127 934.4108 934.4145 -3.86 0 5 0.56 +1Score > 15 indicates identity TDGTWEAR
3924 711.3232 1420.6318 1420.6201 8.29 1 5 0.72 +1Score > 16 indicates identity HSQTHCAHCRK
660 441.1923 880.3700 880.3562 15.7 0 5 0.39 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
5877 873.3969 1744.7792 1744.7839 -2.64 1 5 1 +1Score > 18 indicates identity GGQYCSADYQAQLKR + Deamidated (NQ)
4284 738.3355 1474.6564 1474.6324 16.3 0 5 0.58 +1Score > 15 indicates identity QHQQFADQESVR + 3 Deamidated (NQ)
769 441.2004 880.3862 880.3927 -7.31 0 5 0.49 +1Score > 15 indicates identity DGDGFGVSK
5960 873.3969 1744.7792 1744.7976 -10.5 0 5 1.1 +1Score > 18 indicates identity GTAVNSDGASNGLSAPNGR + Deamidated (NQ)
2270 562.7557 1123.4968 1123.4894 6.65 0 5 0.47 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
5945 873.3969 1744.7792 1744.7735 3.28 0 5 1.1 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
1080 468.2127 934.4108 934.4178 -7.46 1 5 0.58 +1Score > 15 indicates identity LDEDCKR
443 414.1881 826.3616 826.3677 -7.33 0 5 0.36 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
3640 684.3109 1366.6072 1366.6252 -13.1 0 5 0.69 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
4097 724.8293 1447.6440 1447.6725 -19.7 1 5 0.39 +1Score > 13 indicates identity ECAAEITDKAGQR
751 441.2004 880.3862 880.3862 0.11 0 5 0.51 +1Score > 15 indicates identity GFCAGVDR
4300 738.3355 1474.6564 1474.6663 -6.70 0 5 0.62 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
6756 940.9276 1879.8406 1879.8258 7.90 0 5 0.96 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
6866 954.4337 1906.8528 1906.8784 -13.4 0 5 0.93 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
3480 670.8048 1339.5950 1339.6116 -12.4 0 5 0.77 +1Score > 16 indicates identity IQGQSGHSSDPAR + Deamidated (NQ)
1060 468.2127 934.4108 934.4178 -7.46 1 5 0.61 +1Score > 15 indicates identity LDEDCKR
6851 954.4337 1906.8528 1906.8784 -13.4 0 5 0.93 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
5590 846.3846 1690.7546 1690.7807 -15.4 1 5 0.68 +1Score > 16 indicates identity EEVVGAEMMRHFEK
3616 684.3109 1366.6072 1366.6299 -16.6 1 5 0.72 +1Score > 16 indicates identity GAAMEGQYKGAQR + Deamidated (NQ)
1071 468.2127 934.4108 934.4178 -7.46 1 5 0.62 +1Score > 15 indicates identity LDEDCKR
2846 616.7802 1231.5458 1231.5615 -12.7 1 5 0.58 +1Score > 15 indicates identity IREMSDNDPR
3575 670.8048 1339.5950 1339.6112 -12.0 0 5 0.8 +1Score > 16 indicates identity ASVSMQMGIQVR + 2 Deamidated (NQ); 2 Oxidation (M)
4459 751.8416 1501.6686 1501.6943 -17.1 1 5 0.91 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
5417 832.8785 1663.7424 1663.7406 1.10 1 5 0.71 +1Score > 16 indicates identity MDPSLNTMANDRQR + Oxidation (M)
6864 954.4337 1906.8528 1906.8784 -13.4 0 5 0.98 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
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