MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1138 468.2127 934.4108
1139 468.2127 934.4108
1140 468.2127 934.4108
1141 468.2127 934.4108
1142 481.7146 961.4146
1143 481.7181 961.4216
1144 481.7188 961.4230
1145 481.7188 961.4230
1146 481.7188 961.4230
1147 481.7188 961.4230
1148 481.7188 961.4230
1149 481.7188 961.4230
1150 481.7188 961.4230
1151 481.7188 961.4230
1152 481.7188 961.4230
1153 481.7188 961.4230
1154 481.7188 961.4230
1155 481.7188 961.4230
1156 481.7188 961.4230
1157 481.7188 961.4230
1158 481.7188 961.4230
1159 481.7188 961.4230
1160 481.7188 961.4230
1161 481.7188 961.4230
1162 481.7188 961.4230
1163 481.7188 961.4230
1164 481.7188 961.4230
1165 481.7188 961.4230
1166 481.7188 961.4230
1167 481.7188 961.4230
1168 481.7188 961.4230
1169 481.7188 961.4230
1170 481.7188 961.4230
1171 481.7188 961.4230
1172 481.7188 961.4230
1174 481.7188 961.4230
1175 481.7188 961.4230
1176 481.7188 961.4230
1177 481.7188 961.4230
1178 481.7188 961.4230
1179 481.7188 961.4230
1180 481.7188 961.4230
1181 481.7188 961.4230
1183 481.7188 961.4230
1184 481.7188 961.4230
1186 481.7188 961.4230
1187 481.7188 961.4230
1188 481.7188 961.4230
1189 481.7188 961.4230
1190 481.7188 961.4230
1192 481.7188 961.4230
1193 481.7188 961.4230
1194 481.7188 961.4230
1195 481.7188 961.4230
1196 481.7188 961.4230
1197 481.7188 961.4230
1198 481.7188 961.4230
1199 481.7188 961.4230
1200 481.7188 961.4230
1201 481.7188 961.4230
1202 481.7188 961.4230
1203 481.7188 961.4230
1204 481.7188 961.4230
1205 481.7188 961.4230
1206 481.7188 961.4230
1207 481.7188 961.4230
1208 481.7188 961.4230
1209 481.7188 961.4230
1210 481.7188 961.4230
1212 481.7188 961.4230
1213 481.7188 961.4230
1214 481.7188 961.4230
1215 481.7188 961.4230
1216 481.7188 961.4230
1217 481.7188 961.4230
1218 481.7188 961.4230
1219 481.7188 961.4230
1220 481.7188 961.4230
1221 481.7188 961.4230
1222 481.7188 961.4230
1223 481.7188 961.4230
1224 481.7188 961.4230
1225 481.7188 961.4230
1226 481.7188 961.4230
1228 481.7188 961.4230
1229 481.7188 961.4230
1230 481.7188 961.4230
1231 481.7188 961.4230
1232 481.7188 961.4230
1233 481.7188 961.4230
1234 481.7188 961.4230
1235 481.7188 961.4230
1236 481.7188 961.4230
1237 481.7188 961.4230
1238 481.7188 961.4230
1239 481.7188 961.4230
1240 481.7188 961.4230
1241 481.7188 961.4230
1242 481.7188 961.4230
1243 481.7188 961.4230
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