MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
991 468.2127 934.4108
992 468.2127 934.4108
993 468.2127 934.4108
994 468.2127 934.4108
995 468.2127 934.4108
996 468.2127 934.4108
997 468.2127 934.4108
998 468.2127 934.4108
999 468.2127 934.4108
1000 468.2127 934.4108
1001 468.2127 934.4108
1002 468.2127 934.4108
1003 468.2127 934.4108
1004 468.2127 934.4108
1005 468.2127 934.4108
1006 468.2127 934.4108
1007 468.2127 934.4108
1008 468.2127 934.4108
1009 468.2127 934.4108
1010 468.2127 934.4108
1011 468.2127 934.4108
1012 468.2127 934.4108
1013 468.2127 934.4108
1014 468.2127 934.4108
1015 468.2127 934.4108
1016 468.2127 934.4108
1017 468.2127 934.4108
1018 468.2127 934.4108
1019 468.2127 934.4108
1020 468.2127 934.4108
1021 468.2127 934.4108
1028 468.2127 934.4108
1029 468.2127 934.4108
1033 468.2127 934.4108
1044 468.2127 934.4108
1047 468.2127 934.4108
1048 468.2127 934.4108
1054 468.2127 934.4108
1055 468.2127 934.4108
1056 468.2127 934.4108
1057 468.2127 934.4108
1058 468.2127 934.4108
1061 468.2127 934.4108
1063 468.2127 934.4108
1064 468.2127 934.4108
1066 468.2127 934.4108
1067 468.2127 934.4108
1069 468.2127 934.4108
1070 468.2127 934.4108
1072 468.2127 934.4108
1073 468.2127 934.4108
1075 468.2127 934.4108
1076 468.2127 934.4108
1077 468.2127 934.4108
1078 468.2127 934.4108
1082 468.2127 934.4108
1084 468.2127 934.4108
1085 468.2127 934.4108
1086 468.2127 934.4108
1087 468.2127 934.4108
1090 468.2127 934.4108
1091 468.2127 934.4108
1092 468.2127 934.4108
1093 468.2127 934.4108
1094 468.2127 934.4108
1096 468.2127 934.4108
1097 468.2127 934.4108
1098 468.2127 934.4108
1099 468.2127 934.4108
1100 468.2127 934.4108
1102 468.2127 934.4108
1104 468.2127 934.4108
1105 468.2127 934.4108
1108 468.2127 934.4108
1110 468.2127 934.4108
1111 468.2127 934.4108
1112 468.2127 934.4108
1113 468.2127 934.4108
1114 468.2127 934.4108
1115 468.2127 934.4108
1116 468.2127 934.4108
1117 468.2127 934.4108
1118 468.2127 934.4108
1119 468.2127 934.4108
1120 468.2127 934.4108
1122 468.2127 934.4108
1123 468.2127 934.4108
1124 468.2127 934.4108
1125 468.2127 934.4108
1126 468.2127 934.4108
1128 468.2127 934.4108
1129 468.2127 934.4108
1130 468.2127 934.4108
1131 468.2127 934.4108
1132 468.2127 934.4108
1133 468.2127 934.4108
1134 468.2127 934.4108
1135 468.2127 934.4108
1136 468.2127 934.4108
1137 468.2127 934.4108
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