MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
878 454.7066 907.3986
879 454.7066 907.3986
880 454.7066 907.3986
881 454.7066 907.3986
882 454.7066 907.3986
883 454.7066 907.3986
885 454.7066 907.3986
886 454.7066 907.3986
887 454.7066 907.3986
888 454.7066 907.3986
890 454.7066 907.3986
891 454.7066 907.3986
892 454.7066 907.3986
893 454.7066 907.3986
894 454.7066 907.3986
895 454.7066 907.3986
896 454.7066 907.3986
897 454.7066 907.3986
898 454.7066 907.3986
899 454.7066 907.3986
900 454.7066 907.3986
901 454.7066 907.3986
902 454.7066 907.3986
903 454.7066 907.3986
904 454.7066 907.3986
905 454.7066 907.3986
906 454.7066 907.3986
907 454.7066 907.3986
908 454.7066 907.3986
909 454.7066 907.3986
910 454.7066 907.3986
911 454.7066 907.3986
912 454.7066 907.3986
913 454.7066 907.3986
914 454.7066 907.3986
915 454.7066 907.3986
916 454.7066 907.3986
917 454.7066 907.3986
918 454.7066 907.3986
919 454.7066 907.3986
920 454.7066 907.3986
921 454.7066 907.3986
923 454.7066 907.3986
924 454.7066 907.3986
925 454.7066 907.3986
926 454.7066 907.3986
927 454.7066 907.3986
928 454.7066 907.3986
929 454.7066 907.3986
930 454.7066 907.3986
931 454.7066 907.3986
932 454.7066 907.3986
933 454.7066 907.3986
934 454.7066 907.3986
935 454.7066 907.3986
936 454.7066 907.3986
937 454.7066 907.3986
938 454.7066 907.3986
939 454.7066 907.3986
940 454.7066 907.3986
941 454.7066 907.3986
942 454.7066 907.3986
943 454.7066 907.3986
944 454.7066 907.3986
945 454.7066 907.3986
946 454.7066 907.3986
947 454.7066 907.3986
948 454.7066 907.3986
949 454.7066 907.3986
950 454.7066 907.3986
951 454.7066 907.3986
952 454.7066 907.3986
953 454.7066 907.3986
954 454.7066 907.3986
955 454.7066 907.3986
956 454.7066 907.3986
957 454.7066 907.3986
958 454.7066 907.3986
959 454.7066 907.3986
960 454.7066 907.3986
961 454.7066 907.3986
963 454.7066 907.3986
964 454.7066 907.3986
965 454.7066 907.3986
966 454.7066 907.3986
967 454.7066 907.3986
968 454.7066 907.3986
969 454.7066 907.3986
970 454.7066 907.3986
972 454.7066 907.3986
973 454.7066 907.3986
974 454.7066 907.3986
978 454.7068 907.3990
979 468.2070 934.3994
980 468.2127 934.4108
981 468.2127 934.4108
982 468.2127 934.4108
985 468.2127 934.4108
986 468.2127 934.4108
990 468.2127 934.4108
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