MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
630 427.6943 853.3740
631 427.6943 853.3740
632 427.6943 853.3740
633 427.6943 853.3740
634 427.6943 853.3740
635 427.6943 853.3740
636 427.6943 853.3740
637 427.6943 853.3740
638 427.6943 853.3740
639 427.6943 853.3740
641 427.6943 853.3740
642 427.6943 853.3740
643 427.6943 853.3740
644 427.6943 853.3740
645 427.6943 853.3740
646 427.6943 853.3740
647 427.6943 853.3740
648 427.6943 853.3740
649 427.6943 853.3740
650 427.6943 853.3740
651 427.6943 853.3740
652 427.6943 853.3740
664 441.1925 880.3704
665 441.1925 880.3704
667 441.1925 880.3704
668 441.1926 880.3706
669 441.1926 880.3706
670 441.1927 880.3708
671 441.1928 880.3710
673 441.1928 880.3710
674 441.1928 880.3710
676 441.1930 880.3714
677 441.1931 880.3716
679 441.1932 880.3718
681 441.1932 880.3718
682 441.1932 880.3718
683 441.1933 880.3720
688 441.1935 880.3724
690 441.1935 880.3724
696 441.1938 880.3730
697 441.1939 880.3732
699 441.1939 880.3732
700 441.1940 880.3734
702 441.1940 880.3734
703 441.1940 880.3734
705 441.1941 880.3736
707 441.1942 880.3738
708 441.1943 880.3740
709 441.1943 880.3740
710 441.1945 880.3744
712 441.1946 880.3746
713 441.1947 880.3748
714 441.1948 880.3750
715 441.1948 880.3750
716 441.1949 880.3752
717 441.1950 880.3754
718 441.1950 880.3754
719 441.1951 880.3756
720 441.1951 880.3756
721 441.1954 880.3762
722 441.1955 880.3764
723 441.1962 880.3778
724 441.1990 880.3834
725 441.1993 880.3840
726 441.2000 880.3854
727 441.2004 880.3862
728 441.2004 880.3862
729 441.2004 880.3862
730 441.2004 880.3862
731 441.2004 880.3862
732 441.2004 880.3862
733 441.2004 880.3862
734 441.2004 880.3862
735 441.2004 880.3862
736 441.2004 880.3862
737 441.2004 880.3862
738 441.2004 880.3862
739 441.2004 880.3862
740 441.2004 880.3862
741 441.2004 880.3862
744 441.2004 880.3862
745 441.2004 880.3862
746 441.2004 880.3862
747 441.2004 880.3862
748 441.2004 880.3862
749 441.2004 880.3862
750 441.2004 880.3862
752 441.2004 880.3862
753 441.2004 880.3862
754 441.2004 880.3862
755 441.2004 880.3862
757 441.2004 880.3862
758 441.2004 880.3862
759 441.2004 880.3862
761 441.2004 880.3862
762 441.2004 880.3862
763 441.2004 880.3862
764 441.2004 880.3862
765 441.2004 880.3862
767 441.2004 880.3862
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