MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
274 400.6820 799.3494
275 400.6820 799.3494
276 400.6820 799.3494
277 400.6820 799.3494
278 400.6820 799.3494
279 400.6820 799.3494
280 400.6820 799.3494
281 400.6820 799.3494
282 400.6820 799.3494
283 400.6820 799.3494
284 400.6820 799.3494
285 400.6820 799.3494
286 400.6820 799.3494
287 400.6820 799.3494
288 400.6820 799.3494
289 400.6820 799.3494
290 400.6820 799.3494
291 400.6820 799.3494
292 400.6820 799.3494
293 400.6820 799.3494
294 400.6820 799.3494
295 400.6820 799.3494
296 400.6820 799.3494
297 400.6820 799.3494
298 400.6820 799.3494
299 400.6820 799.3494
300 400.6820 799.3494
301 400.6820 799.3494
302 400.6820 799.3494
303 400.6820 799.3494
304 400.6820 799.3494
305 400.6820 799.3494
306 400.6820 799.3494
307 400.6820 799.3494
308 400.6820 799.3494
309 400.6820 799.3494
310 400.6820 799.3494
311 400.6820 799.3494
312 400.6820 799.3494
313 400.6820 799.3494
314 400.6820 799.3494
315 400.6820 799.3494
316 400.6820 799.3494
317 400.6820 799.3494
318 400.6820 799.3494
319 400.6820 799.3494
320 400.6820 799.3494
321 400.6820 799.3494
322 400.6820 799.3494
323 400.6820 799.3494
324 400.6821 799.3496
325 400.6822 799.3498
326 400.6829 799.3512
327 414.1803 826.3460
328 414.1805 826.3464
329 414.1808 826.3470
330 414.1810 826.3474
331 414.1816 826.3486
333 414.1819 826.3492
334 414.1819 826.3492
335 414.1820 826.3494
337 414.1821 826.3496
338 414.1823 826.3500
339 414.1824 826.3502
340 414.1825 826.3504
341 414.1825 826.3504
342 414.1825 826.3504
343 414.1828 826.3510
344 414.1828 826.3510
345 414.1828 826.3510
346 414.1829 826.3512
347 414.1831 826.3516
349 414.1832 826.3518
350 414.1834 826.3522
352 414.1835 826.3524
353 414.1835 826.3524
354 414.1836 826.3526
355 414.1837 826.3528
356 414.1837 826.3528
357 414.1838 826.3530
358 414.1838 826.3530
360 414.1841 826.3536
362 414.1841 826.3536
364 414.1843 826.3540
365 414.1844 826.3542
366 414.1846 826.3546
367 414.1847 826.3548
369 414.1848 826.3550
370 414.1848 826.3550
373 414.1850 826.3554
374 414.1851 826.3556
376 414.1852 826.3558
378 414.1858 826.3570
380 414.1859 826.3572
381 414.1868 826.3590
382 414.1872 826.3598
383 414.1881 826.3616
384 414.1881 826.3616
385 414.1881 826.3616
386 414.1881 826.3616
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