MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
171 400.6820 799.3494
172 400.6820 799.3494
173 400.6820 799.3494
174 400.6820 799.3494
175 400.6820 799.3494
176 400.6820 799.3494
177 400.6820 799.3494
178 400.6820 799.3494
180 400.6820 799.3494
181 400.6820 799.3494
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186 400.6820 799.3494
187 400.6820 799.3494
188 400.6820 799.3494
189 400.6820 799.3494
190 400.6820 799.3494
191 400.6820 799.3494
192 400.6820 799.3494
193 400.6820 799.3494
194 400.6820 799.3494
195 400.6820 799.3494
196 400.6820 799.3494
197 400.6820 799.3494
198 400.6820 799.3494
199 400.6820 799.3494
200 400.6820 799.3494
201 400.6820 799.3494
202 400.6820 799.3494
203 400.6820 799.3494
204 400.6820 799.3494
205 400.6820 799.3494
206 400.6820 799.3494
207 400.6820 799.3494
208 400.6820 799.3494
209 400.6820 799.3494
210 400.6820 799.3494
211 400.6820 799.3494
212 400.6820 799.3494
213 400.6820 799.3494
214 400.6820 799.3494
215 400.6820 799.3494
216 400.6820 799.3494
217 400.6820 799.3494
218 400.6820 799.3494
219 400.6820 799.3494
220 400.6820 799.3494
221 400.6820 799.3494
222 400.6820 799.3494
223 400.6820 799.3494
224 400.6820 799.3494
225 400.6820 799.3494
226 400.6820 799.3494
227 400.6820 799.3494
228 400.6820 799.3494
229 400.6820 799.3494
230 400.6820 799.3494
231 400.6820 799.3494
232 400.6820 799.3494
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234 400.6820 799.3494
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238 400.6820 799.3494
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240 400.6820 799.3494
241 400.6820 799.3494
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243 400.6820 799.3494
244 400.6820 799.3494
245 400.6820 799.3494
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247 400.6820 799.3494
248 400.6820 799.3494
249 400.6820 799.3494
250 400.6820 799.3494
251 400.6820 799.3494
252 400.6820 799.3494
253 400.6820 799.3494
254 400.6820 799.3494
255 400.6820 799.3494
258 400.6820 799.3494
259 400.6820 799.3494
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263 400.6820 799.3494
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266 400.6820 799.3494
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268 400.6820 799.3494
269 400.6820 799.3494
270 400.6820 799.3494
271 400.6820 799.3494
272 400.6820 799.3494
273 400.6820 799.3494
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