MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
62 387.1759 772.3372
63 387.1759 772.3372
64 387.1759 772.3372
66 387.1781 772.3416
67 387.1781 772.3416
68 387.1785 772.3424
69 387.1786 772.3426
70 387.1786 772.3426
71 387.1787 772.3428
72 387.1789 772.3432
73 387.1789 772.3432
74 387.1792 772.3438
75 387.1794 772.3442
76 387.1794 772.3442
78 387.1795 772.3444
79 387.1797 772.3448
80 387.1797 772.3448
81 387.1798 772.3450
82 387.1798 772.3450
83 387.1798 772.3450
84 387.1799 772.3452
85 387.1800 772.3454
86 387.1800 772.3454
88 387.1801 772.3456
89 387.1801 772.3456
90 387.1801 772.3456
91 387.1801 772.3456
92 387.1801 772.3456
93 387.1802 772.3458
94 387.1802 772.3458
95 387.1802 772.3458
96 387.1803 772.3460
97 387.1803 772.3460
98 387.1803 772.3460
99 387.1803 772.3460
100 387.1803 772.3460
103 387.1804 772.3462
104 387.1804 772.3462
105 387.1805 772.3464
106 387.1805 772.3464
107 387.1805 772.3464
108 387.1805 772.3464
109 387.1805 772.3464
110 387.1805 772.3464
111 387.1805 772.3464
112 387.1806 772.3466
113 387.1806 772.3466
114 387.1806 772.3466
116 387.1806 772.3466
117 387.1806 772.3466
118 387.1807 772.3468
119 387.1807 772.3468
120 387.1807 772.3468
121 387.1808 772.3470
122 387.1808 772.3470
123 387.1808 772.3470
124 387.1808 772.3470
125 387.1808 772.3470
126 387.1808 772.3470
127 387.1809 772.3472
129 387.1810 772.3474
130 387.1810 772.3474
131 387.1811 772.3476
132 387.1811 772.3476
133 387.1811 772.3476
134 387.1812 772.3478
135 387.1812 772.3478
136 387.1812 772.3478
137 387.1812 772.3478
138 387.1812 772.3478
139 387.1813 772.3480
140 387.1813 772.3480
141 387.1813 772.3480
143 387.1814 772.3482
144 387.1815 772.3484
145 387.1815 772.3484
146 387.1815 772.3484
147 387.1815 772.3484
148 387.1815 772.3484
149 387.1816 772.3486
150 387.1816 772.3486
151 387.1816 772.3486
153 387.1817 772.3488
154 387.1818 772.3490
155 387.1819 772.3492
156 387.1819 772.3492
157 387.1819 772.3492
158 387.1819 772.3492
159 387.1820 772.3494
160 387.1821 772.3496
161 387.1826 772.3506
162 387.1827 772.3508
163 387.1830 772.3514
164 400.6763 799.3380
165 400.6820 799.3494
166 400.6820 799.3494
167 400.6820 799.3494
168 400.6820 799.3494
169 400.6820 799.3494
170 400.6820 799.3494
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