MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_1
MS data file : PRT1377_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:28:41 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,307

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 7290)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6452 913.9153 1825.8160 1825.7900 14.2 0 14 0.12 +1Score > 17 indicates identity NGYNSAALNGDMNQALR + 2 Deamidated (NQ); Oxidation (M)
2096 549.2496 1096.4846 1096.4978 -12.0 0 13 0.084 +1Score > 15 indicates identity YYGYTGAFR
2432 576.2618 1150.5090 1150.5288 -17.2 0 13 0.095 +1Score > 15 indicates identity QTQLTEEMR + Oxidation (M)
976 454.7066 907.3986 907.3957 3.29 0 13 0.071 +1Score > 14 indicates identity ELMEGNAK + Deamidated (NQ); Oxidation (M)
977 454.7066 907.3986 907.3957 3.29 0 13 0.071 +1Score > 14 indicates identity ELMEGNAK + Deamidated (NQ); Oxidation (M)
3789 697.8171 1393.6196 1393.6217 -1.50 0 13 0.13 +1Score > 16 indicates identity SMQDPIADMLTR + Deamidated (NQ); Oxidation (M)
975 454.7066 907.3986 907.3957 3.29 0 13 0.073 +1Score > 14 indicates identity ELMEGNAK + Deamidated (NQ); Oxidation (M)
6579 927.4214 1852.8282 1852.8625 -18.5 1 13 0.14 +1Score > 17 indicates identity NQKIGSLGMDVYENER + Deamidated (NQ)
2776 616.7802 1231.5458 1231.5429 2.42 1 12 0.099 +1Score > 15 indicates identity REEEEQQQR + Deamidated (NQ)
2428 576.2618 1150.5090 1150.5288 -17.2 0 12 0.12 +1Score > 15 indicates identity QTQLTEEMR + Oxidation (M)
2734 603.2741 1204.5336 1204.5394 -4.75 1 12 0.14 +1Score > 16 indicates identity EAAASEKDPCK
1963 549.2496 1096.4846 1096.4753 8.49 1 12 0.11 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
6434 913.9153 1825.8160 1825.7900 14.2 0 11 0.21 +1Score > 17 indicates identity NGYNSAALNGDMNQALR + 2 Deamidated (NQ); Oxidation (M)
4126 724.8293 1447.6440 1447.6249 13.2 0 11 0.094 +1Score > 13 indicates identity ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M)
406 414.1881 826.3616 826.3643 -3.23 0 11 0.087 +1Score > 13 indicates identity MYVNQR + Deamidated (NQ); Oxidation (M)
519 427.6943 853.3740 853.3851 -13.0 0 11 0.076 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
2110 549.2496 1096.4846 1096.4753 8.49 1 11 0.13 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
1081 468.2127 934.4108 934.4178 -7.46 1 11 0.15 +1Score > 15 indicates identity LDEDCKR
41 387.1759 772.3372 772.3351 2.72 0 11 0.078 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
3563 670.8048 1339.5950 1339.6125 -13.0 1 11 0.19 +1Score > 16 indicates identity AANQAFMMERR + Oxidation (M)
3661 684.3109 1366.6072 1366.6252 -13.1 0 11 0.18 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
2712 603.2741 1204.5336 1204.5394 -4.75 0 11 0.18 +1Score > 16 indicates identity ADCEQLAELR + Deamidated (NQ)
426 414.1881 826.3616 826.3677 -7.33 0 11 0.095 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
859 454.7066 907.3986 907.3817 18.6 1 11 0.11 +1Score > 14 indicates identity ENMGRER + Deamidated (NQ); Oxidation (M)
2976 630.2864 1258.5582 1258.5789 -16.4 0 11 0.11 +1Score > 14 indicates identity DDGLILNDNGGR + Deamidated (NQ)
368 414.1847 826.3548 826.3677 -15.6 0 11 0.097 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
4209 724.8293 1447.6440 1447.6725 -19.7 1 11 0.11 -1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
2018 549.2496 1096.4846 1096.4753 8.49 1 11 0.15 +1Score > 15 indicates identity GDARMMASSR + Oxidation (M)
5290 819.3723 1636.7300 1636.7119 11.1 1 10 0.22 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
427 414.1881 826.3616 826.3677 -7.33 0 10 0.11 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
3734 684.3109 1366.6072 1366.6252 -13.1 0 10 0.21 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
6582 927.4214 1852.8282 1852.8625 -18.5 1 10 0.25 +1Score > 17 indicates identity NQKIGSLGMDVYENER + Deamidated (NQ)
6330 900.4092 1798.8038 1798.8196 -8.76 0 10 0.17 +1Score > 15 indicates identity TEGATVFGYQVMDPER
2830 616.7802 1231.5458 1231.5615 -12.7 1 10 0.18 +1Score > 15 indicates identity IREMSDNDPR
6321 900.4092 1798.8038 1798.7870 9.35 1 10 0.18 +1Score > 15 indicates identity QPNGQVWSPQNDDRR + 3 Deamidated (NQ)
1417 495.2250 988.4354 988.4358 -0.35 0 10 0.11 +1Score > 13 indicates identity ISSGGMMFK + 2 Oxidation (M)
407 414.1881 826.3616 826.3677 -7.33 0 10 0.13 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
2318 576.2618 1150.5090 1150.5214 -10.8 0 10 0.21 +1Score > 15 indicates identity NTTEAGATSGSR
530 427.6943 853.3740 853.3851 -13.0 0 9 0.11 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
3728 684.3109 1366.6072 1366.6252 -13.1 0 9 0.26 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
6332 900.4092 1798.8038 1798.8196 -8.76 0 9 0.21 +1Score > 15 indicates identity TEGATVFGYQVMDPER
1966 549.2496 1096.4846 1096.4978 -12.0 0 9 0.2 +1Score > 15 indicates identity YYGYTGAFR
566 427.6943 853.3740 853.3851 -13.0 0 9 0.12 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
655 441.1919 880.3692 880.3562 14.8 0 9 0.16 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
3155 643.7925 1285.5704 1285.5761 -4.41 0 9 0.2 +1Score > 15 indicates identity MQAYFDQLDR
361 414.1841 826.3536 826.3677 -17.0 0 9 0.13 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
2522 589.7679 1177.5212 1177.5298 -7.29 0 9 0.18 +1Score > 14 indicates identity MPQHDQLHR + Deamidated (NQ); Oxidation (M)
1121 468.2127 934.4108 934.4178 -7.46 1 9 0.24 +1Score > 15 indicates identity LDEDCKR
6854 954.4337 1906.8528 1906.8784 -13.4 0 9 0.37 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
1109 468.2127 934.4108 934.4178 -7.46 1 9 0.24 +1Score > 15 indicates identity LDEDCKR
1520 508.7311 1015.4476 1015.4611 -13.2 0 9 0.15 +1Score > 13 indicates identity YYDAETVR
1971 549.2496 1096.4846 1096.4978 -12.0 0 9 0.22 +1Score > 15 indicates identity YYGYTGAFR
5357 819.3723 1636.7300 1636.7403 -6.24 0 9 0.32 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
5358 819.3723 1636.7300 1636.7403 -6.24 0 9 0.33 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
351 414.1835 826.3524 826.3677 -18.5 0 9 0.14 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1860 535.7434 1069.4722 1069.4862 -13.1 0 9 0.14 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
654 441.1917 880.3688 880.3562 14.3 0 9 0.18 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
1185 481.7188 961.4230 961.4141 9.31 0 9 0.22 +1Score > 15 indicates identity NGWENNVK + 2 Deamidated (NQ)
2057 549.2496 1096.4846 1096.4978 -12.0 0 9 0.24 +1Score > 15 indicates identity YYGYTGAFR
375 414.1852 826.3558 826.3677 -14.3 0 9 0.16 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
6140 886.9030 1771.7914 1771.7909 0.29 0 8 0.32 +1Score > 16 indicates identity EIVFMGTPEFAAECR + Oxidation (M)
447 414.1881 826.3616 826.3677 -7.33 0 8 0.16 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
4191 724.8293 1447.6440 1447.6725 -19.7 1 8 0.18 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
691 441.1935 880.3724 880.3562 18.4 0 8 0.18 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
4742 778.8539 1555.6932 1555.7235 -19.5 1 8 0.28 +1Score > 15 indicates identity CAAGTGRFLDVMSR + Oxidation (M)
4836 778.8539 1555.6932 1555.6759 11.2 0 8 0.29 +1Score > 15 indicates identity LNAMGAFAEAQTCR + Deamidated (NQ); Oxidation (M)
3432 670.8048 1339.5950 1339.6165 -16.0 1 8 0.37 +1Score > 16 indicates identity AMQWQMDFRK
1065 468.2127 934.4108 934.4178 -7.46 1 8 0.29 +1Score > 15 indicates identity LDEDCKR
1691 522.2372 1042.4598 1042.4607 -0.84 0 8 0.16 +1Score > 13 indicates identity YENFDDIK
685 441.1934 880.3722 880.3562 18.2 0 8 0.2 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
4452 751.8416 1501.6686 1501.6727 -2.71 1 8 0.43 +1Score > 17 indicates identity RMPAMMNYTAGVK + Deamidated (NQ); 2 Oxidation (M)
2030 549.2496 1096.4846 1096.4978 -12.0 0 8 0.28 +1Score > 15 indicates identity YYGYTGAFR
4305 738.3355 1474.6564 1474.6663 -6.70 0 8 0.32 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
4391 738.3372 1474.6598 1474.6582 1.08 1 8 0.32 +1Score > 15 indicates identity AECQNQQGNLRR + 2 Deamidated (NQ)
5012 792.3600 1582.7054 1582.6987 4.26 0 8 0.27 +1Score > 15 indicates identity WEVHNFTMGYQR + Oxidation (M)
4089 724.8293 1447.6440 1447.6249 13.2 0 8 0.21 +1Score > 13 indicates identity ENSMLPEAEQQR + Deamidated (NQ); Oxidation (M)
6709 940.9276 1879.8406 1879.8305 5.41 1 8 0.5 +1Score > 17 indicates identity QSHGNPLMKQGHQMQR + 4 Deamidated (NQ)
6155 886.9030 1771.7914 1771.7909 0.29 0 8 0.38 +1Score > 16 indicates identity EIVFMGTPEFAAECR + Oxidation (M)
693 441.1937 880.3728 880.3562 18.9 0 8 0.21 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
1845 535.7434 1069.4722 1069.4862 -13.1 0 8 0.17 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
687 441.1935 880.3724 880.3562 18.4 0 8 0.21 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
1684 522.2372 1042.4598 1042.4432 16.0 1 8 0.18 +1Score > 13 indicates identity MMMMQSRK + Deamidated (NQ)
2048 549.2496 1096.4846 1096.4978 -12.0 0 7 0.3 +1Score > 15 indicates identity YYGYTGAFR
4919 792.3600 1582.7054 1582.6987 4.26 0 7 0.3 +1Score > 15 indicates identity WEVHNFTMGYQR + Oxidation (M)
6757 940.9276 1879.8406 1879.8687 -14.9 0 7 0.55 +1Score > 17 indicates identity LSDQEIEQTLQAFEAR + 3 Deamidated (NQ)
1079 468.2127 934.4108 934.4178 -7.46 1 7 0.35 +1Score > 15 indicates identity LDEDCKR
546 427.6943 853.3740 853.3851 -13.0 0 7 0.19 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
5726 859.8907 1717.7668 1717.7875 -12.0 1 7 0.35 +1Score > 15 indicates identity AEDMAAANAMASHLRK + 2 Oxidation (M)
4504 751.8416 1501.6686 1501.6718 -2.13 0 7 0.51 +1Score > 17 indicates identity GMLNELDEEPSPR + Oxidation (M)
1739 522.2372 1042.4598 1042.4576 2.17 0 7 0.19 +1Score > 13 indicates identity GFADAIMMR + 2 Oxidation (M)
4951 792.3600 1582.7054 1582.7045 0.58 1 7 0.32 +1Score > 15 indicates identity CQYETLVENNRR + 2 Deamidated (NQ)
3648 684.3109 1366.6072 1366.6221 -10.8 0 7 0.43 +1Score > 16 indicates identity LANAAITDMQMR + Deamidated (NQ); 2 Oxidation (M)
5222 819.3723 1636.7300 1636.7185 7.05 0 7 0.47 +1Score > 16 indicates identity VGGLSVMCEDAEAAGR + Oxidation (M)
2118 549.2507 1096.4868 1096.4978 -9.98 0 7 0.33 +1Score > 15 indicates identity YYGYTGAFR
5020 792.3600 1582.7054 1582.6975 5.00 0 7 0.32 +1Score > 15 indicates identity QEVCAMMALNMLR + Deamidated (NQ); Oxidation (M)
6426 913.9153 1825.8160 1825.8482 -17.6 1 7 0.55 +1Score > 17 indicates identity EDAQLLYGFNNKQER + 2 Deamidated (NQ)
2053 549.2496 1096.4846 1096.4978 -12.0 0 7 0.34 +1Score > 15 indicates identity YYGYTGAFR
3693 684.3109 1366.6072 1366.6252 -13.1 0 7 0.44 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
359 414.1840 826.3534 826.3677 -17.3 0 7 0.21 +1Score > 13 indicates identity MATVSMR + 2 Oxidation (M)
1022 468.2127 934.4108 934.4032 8.17 0 7 0.38 +1Score > 15 indicates identity GNFQPDEK + Deamidated (NQ)
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