MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | PRT1377_JBEI_1 |
| MS data file | : | PRT1377_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 23 Jan 2026 at 21:28:41 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 7,307 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYS_ECOLI | 37 | Serine--tRNA ligase OS=Escherichia coli (strain K12) GN=serS PE=1 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C10_HUMAN | 28 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YMIA_ECOLI | 17 | Uncharacterized protein YmiA OS=Escherichia coli (strain K12) GN=ymiA PE=1 SV=2 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG6_PSEPK | 15 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PERM_ECOLI | 13 | Putative permease PerM OS=Escherichia coli (strain K12) GN=perM PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 5.1 | PERM_ECOLI |
13 | 39283 | 1 (1) | 1 (1) | 0.08 | |
| Putative permease PerM OS=Escherichia coli (strain K12) GN=perM PE=3 SV=1 | |||||||
1 peptide matches (1 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
D:\Xcalibur\Data\Jennifer\PRT1377 Andy\PRT1377_JBEI_1.rawScore > 13 indicates identity |
|||||||||||
| 4078 |
724.8293 | 1447.6440 | 1447.6298 | 9.85 | 0 | 13 | 0.062 | 1Score > 13 indicates identity |
U | -.MLEMLMQWYR.R + 3 Oxidation (M) | |
| No other peptide matches in query | |||||||||||
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