MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 7572)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
441 414.1881 826.3617 826.3643 -3.15 0 3 2.4 +1Score > 19 indicates identity MYVDGAR + Oxidation (M)
1628 508.7311 2030.8955 2030.8851 5.12 1 3 1.3 +1Score > 16 indicates identity MGNEDNQKQPEELEQNK + Deamidated (NQ)
2121 549.2496 2192.9692 2192.9871 -8.15 0 3 0.67 +1Score > 20 indicates identity
Score > 13 indicates homology
DYYEHVHMIPTMIQLDR + Deamidated (NQ); 2 Oxidation (M)
3467 657.2986 2625.1655 2625.1396 9.84 1 3 2.6 +1Score > 19 indicates identity NMKSIQYPHMPEEGMTEESEVK + 2 Oxidation (M)
5946 859.8907 3435.5339 3435.5551 -6.18 1 3 1.3 +1Score > 16 indicates identity TQMLNKQYEAVERPQQTDAAEFTFAQLEK + 6 Deamidated (NQ); Oxidation (M)
825 441.2004 880.3863 880.3926 -7.15 0 3 2.6 +1Score > 19 indicates identity ANEQYEK
874 454.7066 907.3986 907.3957 3.23 0 3 2.1 +1Score > 19 indicates identity EMNGLAEK + Deamidated (NQ); Oxidation (M)
5111 792.3600 1582.7055 1582.6967 5.59 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
NSSMVLMAGEVAEGR + Deamidated (NQ); 2 Oxidation (M)
3270 643.7925 1285.5705 1285.5833 -9.95 0 3 1.7 +1Score > 18 indicates identity NASHGMVGDLNR + Oxidation (M)
5131 792.3600 1582.7055 1582.7218 -10.3 1 3 0.85 +1Score > 20 indicates identity
Score > 15 indicates homology
VIAQMESDMKEAAK + Deamidated (NQ); 2 Oxidation (M)
60 387.1759 1544.6744 1544.6824 -5.17 1 3 1.4 +1Score > 17 indicates identity CQHCQKNEATIR + Deamidated (NQ)
2079 549.2496 2192.9692 2192.9644 2.20 1 3 0.68 +1Score > 20 indicates identity
Score > 14 indicates homology
IAEEGSHQELMDKNGEYSR + Deamidated (NQ)
5800 846.3846 2536.1319 2536.1308 0.42 1 3 2.2 +1Score > 19 indicates identity AKAEMTAEHNEVDTMIASLEDSK + Deamidated (NQ); Oxidation (M)
2370 562.7557 1123.4968 1123.5002 -3.00 0 3 1.8 +1Score > 18 indicates identity GNMAVAMATGGK + Deamidated (NQ); Oxidation (M)
4202 711.3232 1420.6319 1420.6504 -13.0 0 3 2.6 +1Score > 19 indicates identity DAMDGLSLANQLR + 2 Deamidated (NQ); Oxidation (M)
4357 724.8293 1447.6441 1447.6474 -2.23 1 3 1.6 +1Score > 17 indicates identity NVNDRMNVTDNR + Deamidated (NQ)
5702 832.8785 3327.4848 3327.4765 2.51 1 3 1.5 +1Score > 17 indicates identity FNFANEEDMYAAVGYNGITAAQVANRLTEK + 5 Deamidated (NQ); Oxidation (M)
2103 549.2496 1644.7269 1644.7487 -13.3 0 3 0.74 +1Score > 20 indicates identity
Score > 14 indicates homology
MSATVAGMAINFTGNK + Deamidated (NQ); 2 Oxidation (M)
2245 562.7557 1123.4968 1123.5002 -3.00 0 3 1.8 +1Score > 18 indicates identity GNMAVAMATGGK + Deamidated (NQ); Oxidation (M)
384 414.1881 826.3617 826.3564 6.40 1 3 2.5 +1Score > 19 indicates identity MKMEEK + 2 Oxidation (M)
1086 468.2127 1868.8217 1868.8462 -13.1 0 3 2.4 +1Score > 19 indicates identity TEMLTNNIANANTPGYK + 2 Deamidated (NQ); Oxidation (M)
1938 535.7434 1069.4723 1069.4750 -2.55 1 3 1.5 +1Score > 17 indicates identity MDQFKQEK + Deamidated (NQ); Oxidation (M)
3795 684.3109 2733.2145 2733.2084 2.24 1 3 2.6 +1Score > 19 indicates identity MQDIIYCEGANRFPGVDTEQVMK + Deamidated (NQ); 2 Oxidation (M)
3938 697.8171 1393.6196 1393.6282 -6.19 0 3 1.7 +1Score > 17 indicates identity QLNMSVDQLAQK + 4 Deamidated (NQ); Oxidation (M)
6617 913.9153 3651.6320 3651.6991 -18.4 1 3 2.1 +1Score > 18 indicates identity EFDIITTGDYQLILEDDQELYAYLRNGADEK + 2 Deamidated (NQ)
470 414.1881 1652.7235 1652.7273 -2.35 0 3 2.5 +1Score > 19 indicates identity VDQMMDEGLLDEVK + 2 Oxidation (M)
2829 603.2741 1204.5337 1204.5360 -1.95 0 3 2.5 +1Score > 19 indicates identity QINDTYGHQK + 2 Deamidated (NQ)
2854 603.2741 1204.5337 1204.5394 -4.75 0 3 2.5 +1Score > 19 indicates identity MSGAETIHNTK + Deamidated (NQ); Oxidation (M)
1011 454.7066 907.3986 907.4069 -9.14 1 3 2.2 +1Score > 19 indicates identity QEAERMK + Deamidated (NQ); Oxidation (M)
4601 751.8416 3003.3374 3003.3808 -14.5 1 3 1.2 +1Score > 16 indicates identity RSFDIQDGVIEMGEFSPVVDQSFQEK + Deamidated (NQ); Oxidation (M)
5164 792.3600 3165.4111 3165.3704 12.8 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
WNGLADLKLMNMMGYDAMTFGNHEFDK + Deamidated (NQ); Oxidation (M)
930 454.7066 907.3986 907.3957 3.22 0 3 2.2 +1Score > 19 indicates identity MDQQQLK + 2 Deamidated (NQ); Oxidation (M)
4124 711.3232 2130.9479 2130.9481 -0.083 0 3 2.6 +1Score > 19 indicates identity LYDGESVEGYNSVDVEDLK + Deamidated (NQ)
918 454.7066 1814.7972 1814.8033 -3.34 0 3 2.2 +1Score > 19 indicates identity GFIYIEECEDQNGIK + Deamidated (NQ)
5113 792.3600 2374.0583 2374.0821 -10.0 1 3 0.92 +1Score > 20 indicates identity
Score > 15 indicates homology
QGDPGITQFYLSMEDELMKR + Deamidated (NQ); Oxidation (M)
5576 819.3723 1636.7301 1636.7104 12.0 0 3 2.8 +1Score > 20 indicates identity QEENPAFSQLTDEK + 2 Deamidated (NQ)
7259 954.4337 1906.8529 1906.8255 14.4 0 3 2.3 +1Score > 19 indicates identity FYDMDELTGSLETQSR + Oxidation (M)
3086 630.2864 1258.5582 1258.5396 14.8 0 3 2.7 +1Score > 19 indicates identity GMMEFQEMLK + Oxidation (M)
3598 670.8048 2679.1901 2679.1945 -1.61 1 3 2.1 +1Score > 18 indicates identity DDFYNEYGIRIQGNLSPLNMMR + 2 Deamidated (NQ); 2 Oxidation (M)
6288 886.9030 3543.5830 3543.6538 -20.0 1 3 1.7 +1Score > 17 indicates identity CGLVPVLAENYNKSDNCEDTPEAGYFAIAVVK + Deamidated (NQ)
2403 576.2618 2301.0183 2301.0545 -15.7 0 3 2.8 +1Score > 20 indicates identity DFSDVIMAFSEDPEMLALAGK + Oxidation (M)
4356 724.8293 2895.2883 2895.3419 -18.5 1 3 1.7 +1Score > 17 indicates identity VFELRQDSWTDPDTNVIMPQMSIR + 2 Deamidated (NQ); Oxidation (M)
2204 549.2496 1096.4846 1096.5005 -14.5 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
NMTVKDSMR + Oxidation (M)
901 454.7066 907.3986 907.4035 -5.44 0 3 2.2 +1Score > 19 indicates identity EEAADAFR
1319 481.7188 961.4231 961.4240 -0.88 0 3 1.6 +1Score > 17 indicates identity DELEQAEK + Deamidated (NQ)
1413 495.2250 1482.6531 1482.6296 15.8 0 3 2.4 +1Score > 19 indicates identity EITPYQNAMNQR + 3 Deamidated (NQ); Oxidation (M)
1794 522.2372 1563.6899 1563.7198 -19.1 1 3 2.6 +1Score > 19 indicates identity ASERLNEAMNNGLK + 2 Deamidated (NQ); Oxidation (M)
4588 751.8416 3003.3374 3003.3710 -11.2 0 3 1.3 +1Score > 16 indicates identity HILWENIAVYLYWMYDMLQEDPK + 2 Deamidated (NQ); 2 Oxidation (M)
5126 792.3600 3165.4111 3165.3862 7.86 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
MIEQASMTPGTIFMSSQVTAPTEEDMLR + Deamidated (NQ); 4 Oxidation (M)
5127 792.3600 1582.7055 1582.6967 5.59 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
NSSMVLMAGEVAEGR + Deamidated (NQ); 2 Oxidation (M)
1085 468.2127 934.4109 934.4178 -7.41 1 2 2.4 +1Score > 19 indicates identity MQNKAADR + 2 Deamidated (NQ)
2015 535.7434 2138.9445 2138.9797 -16.4 1 2 1.5 +1Score > 17 indicates identity TFSTFNVYDTVPNDYKAR + 2 Deamidated (NQ)
1734 522.2372 2084.9199 2084.9468 -12.9 0 2 2.6 +1Score > 19 indicates identity EIGMIFQDPMTSLNPTMK + Deamidated (NQ); 2 Oxidation (M)
1904 535.7434 2138.9445 2138.9547 -4.75 1 2 1.5 +1Score > 17 indicates identity IRASQLNGCAFCLNMHTK + 3 Deamidated (NQ); Oxidation (M)
4434 738.3355 2949.3129 2949.3662 -18.0 1 2 0.98 +1Score > 20 indicates identity
Score > 15 indicates homology
INFTLDSRYNMPGISITTNSQNSTTR + 3 Deamidated (NQ); Oxidation (M)
582 427.6943 853.3741 853.3575 19.4 0 2 1.2 +1Score > 16 indicates identity GCMFAPR + Oxidation (M)
2234 562.7557 1123.4968 1123.5002 -3.00 0 2 1.8 +1Score > 18 indicates identity GMGIIGMEER + 2 Oxidation (M)
2479 576.2618 1150.5091 1150.5176 -7.33 1 2 2.8 +1Score > 20 indicates identity AGEKLNEDMK + Deamidated (NQ); Oxidation (M)
6917 927.4214 2779.2425 2779.2581 -5.62 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
FKNASGFQAGNFQLSQSMDAAGMINK + 2 Deamidated (NQ); Oxidation (M)
251 400.6820 1598.6989 1598.7246 -16.1 0 2 1.2 +1Score > 16 indicates identity YNATPSQDAMLSTGK + Oxidation (M)
1417 495.2250 1976.8708 1976.8859 -7.65 1 2 2.5 +1Score > 19 indicates identity LYEEKMTDYPAMALDR + 2 Oxidation (M)
3087 630.2864 1887.8373 1887.8639 -14.1 0 2 2.7 +1Score > 19 indicates identity QFQEVEAVTFQYNQR + 2 Deamidated (NQ)
3868 684.3109 1366.6073 1366.5856 15.8 1 2 2.7 +1Score > 19 indicates identity KMIEMNQGQNR + 3 Deamidated (NQ); Oxidation (M)
6501 900.4092 3597.6076 3597.5857 6.09 1 2 2.4 +1Score > 19 indicates identity HPGMGMQPPQAQQRGLQQMFQPMGAAQQQAGAR + 5 Deamidated (NQ); 2 Oxidation (M)
4775 765.3478 2293.0215 2293.0597 -16.7 0 2 2.8 +1Score > 19 indicates identity QQVDYNDDSIQVLEGLEAVR + 3 Deamidated (NQ)
2508 576.2618 1150.5091 1150.5063 2.45 0 2 2.9 +1Score > 20 indicates identity MNQIQLEEK + 3 Deamidated (NQ); Oxidation (M)
2748 603.2741 1806.8005 1806.8029 -1.32 1 2 2.6 +1Score > 19 indicates identity KAHFMADQCQDQSLK + Deamidated (NQ)
3940 697.8171 1393.6196 1393.6282 -6.19 0 2 1.8 +1Score > 17 indicates identity QLNMSVDQLAQK + 4 Deamidated (NQ); Oxidation (M)
5042 778.8539 1555.6932 1555.7076 -9.23 0 2 2.1 +1Score > 18 indicates identity TSMLEVYNPATGEK + Deamidated (NQ); Oxidation (M)
5227 792.3600 1582.7055 1582.7330 -17.4 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
MNEKSAASEMLQTK + Oxidation (M)
1837 522.2372 1563.6899 1563.6922 -1.48 0 2 2.7 +1Score > 19 indicates identity HPGMGMQPPQAQQR + 2 Deamidated (NQ)
2316 562.7557 2246.9936 2247.0114 -7.91 1 2 1.9 +1Score > 18 indicates identity SKDEFGQLGTSFNEMADSLR + Oxidation (M)
6376 886.9030 3543.5830 3543.6470 -18.1 1 2 1.7 +1Score > 17 indicates identity SFFFDTVENDYGRDFLNVFTENAGLLLENR + 2 Deamidated (NQ)
1583 508.7311 2030.8955 2030.9003 -2.40 1 2 1.3 +1Score > 16 indicates identity YEKMLNTYSHQEETSR + Oxidation (M)
2095 549.2496 1096.4846 1096.4785 5.56 0 2 0.94 +1Score > 20 indicates identity
Score > 15 indicates homology
YTNATDADAR
6504 900.4092 2698.2057 2698.2122 -2.39 1 2 2.4 +1Score > 19 indicates identity NDPMWPYLENKYQTSWNPNTGK + Oxidation (M)
940 454.7066 907.3986 907.4069 -9.16 0 2 2.3 +1Score > 19 indicates identity NMEQTIR + Deamidated (NQ); Oxidation (M)
2400 576.2618 2301.0183 2301.0205 -0.98 1 2 2.9 +1Score > 20 indicates identity LSNEKAYQEINDTQEMIEK + 3 Deamidated (NQ); Oxidation (M)
2439 576.2618 1150.5091 1150.5254 -14.2 1 2 2.9 +1Score > 20 indicates identity ALYNRGVNDE + Deamidated (NQ)
3365 643.7925 2571.1411 2571.1509 -3.82 1 2 1.8 +1Score > 18 indicates identity KMELEDADVLCQYWSDPEVTK + Oxidation (M)
3136 630.2864 1258.5582 1258.5612 -2.37 0 2 2.8 +1Score > 19 indicates identity GTGNMELHLDR + Deamidated (NQ); Oxidation (M)
4358 724.8293 2895.2883 2895.3419 -18.5 1 2 1.7 +1Score > 17 indicates identity VFELRQDSWTDPDTNVIMPQMSIR + 2 Deamidated (NQ); Oxidation (M)
870 454.7066 1814.7972 1814.8257 -15.7 1 2 2.3 +1Score > 19 indicates identity LLDNAFRYADQMGQR + 2 Deamidated (NQ); Oxidation (M)
4433 738.3355 2949.3129 2949.2974 5.26 0 2 0.64 +1Score > 20 indicates identity
Score > 13 indicates homology
TAVSCYPNAGLPDEEGQYHESPQSLAK + 2 Deamidated (NQ)
676 427.6943 1706.7481 1706.7345 8.00 0 2 1.2 +1Score > 16 indicates identity EAALVNYNNTYSMSK + 3 Deamidated (NQ)
922 454.7066 1814.7972 1814.8186 -11.8 0 2 2.3 +1Score > 19 indicates identity GTFTMVFDHYEEVPK + Oxidation (M)
4096 711.3232 2130.9479 2130.9635 -7.33 1 2 2.8 +1Score > 19 indicates identity MDNQVLQAIYEMQKEMK + Deamidated (NQ); 2 Oxidation (M)
4486 738.3355 2211.9847 2212.0072 -10.2 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
AAAYIQFDEEGQQQLWSAR + 2 Deamidated (NQ)
3520 657.2986 1312.5827 1312.5574 19.3 0 2 2.9 +1Score > 19 indicates identity MQTGMSVQEMR + Oxidation (M)
4431 738.3355 2949.3129 2949.2974 5.26 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
TAVSCYPNAGLPDEEGQYHESPQSLAK + 2 Deamidated (NQ)
813 441.2004 880.3863 880.3926 -7.17 0 2 2.8 +1Score > 19 indicates identity GEDELYR
931 454.7066 1814.7972 1814.7815 8.65 0 2 2.3 +1Score > 19 indicates identity QLPSDSSEDFMQMLR + 2 Oxidation (M)
2069 549.2496 1644.7269 1644.7487 -13.3 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
MSATVAGMAINFTGNK + Deamidated (NQ); 2 Oxidation (M)
7129 954.4337 3813.7058 3813.7609 -14.5 1 2 2.4 +1Score > 19 indicates identity NMSFTLDDFLEQLGQVRNMGPLEDLIQMMPGAGK + 3 Deamidated (NQ); Oxidation (M)
7150 954.4337 2860.2793 2860.3233 -15.4 1 2 2.4 +1Score > 19 indicates identity KPAHAAMMLIPEPWNENPYMSKEK + Deamidated (NQ); 3 Oxidation (M)
301 400.6820 1598.6989 1598.6812 11.1 0 2 1.3 +1Score > 16 indicates identity MLMQVMQDPEMAK + 3 Oxidation (M)
380 414.1881 1239.5426 1239.5264 13.1 0 2 2.7 +1Score > 19 indicates identity EEMEFMGPVR + Oxidation (M)
437 414.1881 826.3617 826.3466 18.4 0 2 2.7 +1Score > 19 indicates identity MNMFER
6454 900.4092 2698.2057 2698.2387 -12.2 0 2 2.4 +1Score > 19 indicates identity GSILKPDEMDTLSGNQETAMGVMLK + 2 Deamidated (NQ); 2 Oxidation (M)
2436 576.2618 1150.5091 1150.5077 1.25 0 2 2.9 +1Score > 20 indicates identity NMGDPLFANR + Deamidated (NQ); Oxidation (M)
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