MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5901–6000 (out of 7572)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5735 832.8785 1663.7424
5736 832.8785 1663.7424
5737 832.8785 1663.7424
5738 832.8785 1663.7424
5739 832.8785 1663.7424
5740 832.8785 1663.7424
5741 832.8785 1663.7424
5742 832.8785 1663.7424
5743 832.8785 1663.7424
5744 832.8785 1663.7424
5746 832.8785 1663.7424
5747 832.8785 1663.7424
5749 832.8785 1663.7424
5750 832.8785 1663.7424
5751 832.8785 1663.7424
5752 832.8785 1663.7424
5753 832.8785 1663.7424
5754 832.8785 1663.7424
5755 832.8785 1663.7424
5756 832.8785 1663.7424
5757 832.8785 1663.7424
5758 832.8785 1663.7424
5760 832.8785 1663.7424
5761 832.8785 1663.7424
5762 832.8785 1663.7424
5763 846.3846 1690.7546
5764 846.3846 1690.7546
5765 846.3846 1690.7546
5766 846.3846 1690.7546
5767 846.3846 1690.7546
5768 846.3846 1690.7546
5769 846.3846 1690.7546
5770 846.3846 1690.7546
5771 846.3846 1690.7546
5772 846.3846 1690.7546
5773 846.3846 1690.7546
5774 846.3846 1690.7546
5775 846.3846 1690.7546
5776 846.3846 1690.7546
5777 846.3846 1690.7546
5778 846.3846 1690.7546
5779 846.3846 1690.7546
5781 846.3846 1690.7546
5782 846.3846 1690.7546
5783 846.3846 1690.7546
5784 846.3846 1690.7546
5786 846.3846 1690.7546
5787 846.3846 1690.7546
5788 846.3846 1690.7546
5789 846.3846 1690.7546
5790 846.3846 1690.7546
5791 846.3846 1690.7546
5793 846.3846 1690.7546
5794 846.3846 1690.7546
5802 846.3846 1690.7546
5804 846.3846 1690.7546
5805 846.3846 1690.7546
5806 846.3846 1690.7546
5812 846.3846 1690.7546
5813 846.3846 1690.7546
5815 846.3846 1690.7546
5816 846.3846 1690.7546
5818 846.3846 1690.7546
5819 846.3846 1690.7546
5821 846.3846 1690.7546
5822 846.3846 1690.7546
5824 846.3846 1690.7546
5825 846.3846 1690.7546
5826 846.3846 1690.7546
5827 846.3846 1690.7546
5829 846.3846 1690.7546
5830 846.3846 1690.7546
5831 846.3846 1690.7546
5832 846.3846 1690.7546
5833 846.3846 1690.7546
5834 846.3846 1690.7546
5835 846.3846 1690.7546
5836 846.3846 1690.7546
5837 846.3846 1690.7546
5838 846.3846 1690.7546
5839 846.3846 1690.7546
5840 846.3846 1690.7546
5841 846.3846 1690.7546
5842 846.3846 1690.7546
5843 846.3846 1690.7546
5844 846.3846 1690.7546
5845 846.3846 1690.7546
5846 846.3846 1690.7546
5847 846.3846 1690.7546
5848 846.3846 1690.7546
5849 846.3846 1690.7546
5850 846.3846 1690.7546
5851 846.3846 1690.7546
5852 846.3846 1690.7546
5853 846.3846 1690.7546
5854 846.3846 1690.7546
5856 846.3846 1690.7546
5857 846.3846 1690.7546
5858 846.3846 1690.7546
5859 846.3846 1690.7546
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