MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 7572)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2209 549.2496 1644.7269 1644.6977 17.7 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
GMNWSVEAEYNSLK + 2 Deamidated (NQ); Oxidation (M)
6149 873.3969 2617.1689 2617.1604 3.26 0 4 1.8 +1Score > 19 indicates identity ELGYSAMALTDDEVMYGAVEFYK + Oxidation (M)
1970 535.7434 1069.4723 1069.4750 -2.55 1 4 1.1 +1Score > 17 indicates identity MDQFKQEK + Deamidated (NQ); Oxidation (M)
2100 549.2496 1096.4846 1096.4978 -12.0 0 4 0.58 +1Score > 20 indicates identity
Score > 14 indicates homology
YYGYTGAFR
3700 670.8048 1339.5951 1339.6190 -17.9 1 4 1.5 +1Score > 18 indicates identity KDSGFQMNQLR + Deamidated (NQ); Oxidation (M)
2257 562.7557 2246.9936 2247.0100 -7.28 0 4 1.3 +1Score > 18 indicates identity IGNLQAEDEVIEMNQVEANK + 4 Deamidated (NQ)
3824 684.3109 2733.2145 2733.2625 -17.6 1 4 2 +1Score > 19 indicates identity LSQPDMMNFEEHIRDNISDVITK + 2 Deamidated (NQ)
477 414.1881 1239.5426 1239.5554 -10.3 1 4 1.9 +1Score > 19 indicates identity FQAQEREMGK + Deamidated (NQ); Oxidation (M)
976 454.7066 907.3986 907.3923 6.95 0 4 1.6 +1Score > 19 indicates identity NPQYQEK + 2 Deamidated (NQ)
637 427.6943 853.3741 853.3786 -5.31 1 4 0.89 +1Score > 16 indicates identity QMMKQR + Deamidated (NQ); 2 Oxidation (M)
2688 589.7679 1177.5213 1177.5211 0.21 1 4 1.2 +1Score > 17 indicates identity DSESVDKENR
6211 873.3969 2617.1689 2617.1467 8.49 0 4 1.9 +1Score > 19 indicates identity VNQLSGSIQMAQMMLQMFHAMR + 2 Deamidated (NQ); 4 Oxidation (M)
1058 468.2127 934.4109 934.4290 -19.4 1 4 1.8 +1Score > 19 indicates identity AEGSERMR
283 400.6820 1598.6989 1598.6812 11.1 0 4 0.92 +1Score > 16 indicates identity MLMQVMQDPEMAK + 3 Oxidation (M)
3097 630.2864 1887.8373 1887.8639 -14.1 0 4 2 +1Score > 19 indicates identity QFQEVEAVTFQYNQR + 2 Deamidated (NQ)
6157 873.3969 2617.1689 2617.1467 8.49 0 4 1.9 +1Score > 19 indicates identity VNQLSGSIQMAQMMLQMFHAMR + 2 Deamidated (NQ); 4 Oxidation (M)
2084 549.2496 2192.9692 2192.9792 -4.57 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
NPGVMVMMNPFQPASVPAEK + 2 Deamidated (NQ); 3 Oxidation (M)
2201 549.2496 1644.7269 1644.6977 17.7 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
GMNWSVEAEYNSLK + 2 Deamidated (NQ); Oxidation (M)
3784 684.3109 2733.2145 2733.2084 2.24 1 4 2 +1Score > 19 indicates identity MQDIIYCEGANRFPGVDTEQVMK + Deamidated (NQ); 2 Oxidation (M)
4807 765.3478 3057.3620 3057.3956 -11.0 0 4 2.1 +1Score > 19 indicates identity GFVTDVIENEGLQFDCIMACGPTPMLK + Oxidation (M)
816 441.2004 1320.5795 1320.5980 -14.0 0 4 2 +1Score > 19 indicates identity GECLGLAGESGSGK
1589 508.7311 2030.8955 2030.9288 -16.4 1 4 0.99 +1Score > 16 indicates identity NSMKNSIIQGMLTAFANR + 4 Deamidated (NQ); 2 Oxidation (M)
3761 684.3109 2049.9109 2049.9313 -9.96 1 4 2 +1Score > 19 indicates identity QGEYQKPEPPDSGDMKTK + Oxidation (M)
74 387.1759 1544.6744 1544.6673 4.60 0 4 1.1 +1Score > 17 indicates identity EQMMYDMEALLR + Oxidation (M)
7425 967.9399 1933.8652 1933.8752 -5.16 1 4 1.1 +1Score > 17 indicates identity NGQVQSNEIKNQLNSEK + 5 Deamidated (NQ)
6130 873.3969 2617.1689 2617.1467 8.49 0 4 1.9 +1Score > 19 indicates identity VNQLSGSIQMAQMMLQMFHAMR + 2 Deamidated (NQ); 4 Oxidation (M)
5624 832.8785 1663.7424 1663.7624 -12.0 1 4 1.2 +1Score > 17 indicates identity NRMNGGWETIVNQK + 2 Deamidated (NQ); Oxidation (M)
1797 522.2372 1563.6899 1563.7086 -12.0 1 4 2 +1Score > 19 indicates identity LEKNGEMTEDELR + Deamidated (NQ)
2173 549.2496 1644.7269 1644.7406 -8.35 0 4 0.55 +1Score > 20 indicates identity
Score > 14 indicates homology
QYETFEDQLQTLK + 3 Deamidated (NQ)
2796 603.2741 1204.5337 1204.5459 -10.2 0 4 1.9 +1Score > 19 indicates identity EAGLLDDQSEK + Deamidated (NQ)
6158 873.3969 3489.5585 3489.6122 -15.4 1 4 1.9 +1Score > 19 indicates identity AYPVQGSDCSPFCSLAFFRPDIYHEIKQR + 2 Deamidated (NQ)
7188 954.4337 2860.2793 2860.3185 -13.7 0 4 1.8 +1Score > 19 indicates identity SQQSSDPDPNCVDRPVEGYLAVAVVR + 3 Deamidated (NQ)
390 414.1881 1652.7235 1652.7278 -2.62 1 4 2 +1Score > 19 indicates identity RFNTANDDNVTQVR + 4 Deamidated (NQ)
2271 562.7557 2246.9936 2246.9662 12.2 0 4 1.4 +1Score > 18 indicates identity NLPENGSSSQSGGGEEEAAPLSK + 3 Deamidated (NQ)
4207 711.3232 1420.6319 1420.6218 7.10 1 4 2.1 +1Score > 19 indicates identity EPENQQAFRGSR + 3 Deamidated (NQ)
80 387.1759 1544.6744 1544.6824 -5.17 1 4 1.1 +1Score > 17 indicates identity CQHCQKNEATIR + Deamidated (NQ)
2310 562.7557 1123.4968 1123.4903 5.83 1 4 1.4 +1Score > 18 indicates identity GNMNWMKAR + Deamidated (NQ); Oxidation (M)
5463 819.3723 1636.7301 1636.7515 -13.1 1 4 2.2 +1Score > 20 indicates identity GIAENDAFLKDECR
5798 846.3846 2536.1319 2536.1018 11.9 0 4 1.8 +1Score > 19 indicates identity QMAQSLEQMNVINHVMEASVEK + 5 Deamidated (NQ); Oxidation (M)
1645 508.7311 1015.4477 1015.4393 8.34 1 4 1 +1Score > 16 indicates identity NNSSKMYR + Deamidated (NQ); Oxidation (M)
2020 535.7434 2138.9445 2138.9758 -14.6 0 4 1.2 +1Score > 17 indicates identity NAMGAANAMVAADMALAGITSR + Deamidated (NQ); 2 Oxidation (M)
3250 643.7925 2571.1411 2571.1812 -15.6 1 4 1.4 +1Score > 18 indicates identity QIPGSFIPMQFDNEANPEAHRR + 2 Deamidated (NQ); Oxidation (M)
2441 576.2618 1150.5091 1150.5077 1.25 0 4 2.2 +1Score > 20 indicates identity NMGDPLFANR + Deamidated (NQ); Oxidation (M)
310 400.6820 799.3495 799.3422 9.09 0 4 0.96 +1Score > 16 indicates identity FTMEEK + Oxidation (M)
363 414.1881 1652.7235 1652.7273 -2.31 0 4 2 +1Score > 19 indicates identity SQLMQCAQLINQAK + 5 Deamidated (NQ); Oxidation (M)
647 427.6943 1706.7481 1706.7675 -11.4 0 4 0.94 +1Score > 16 indicates identity DGQYSLSYVDFINGK + 2 Deamidated (NQ)
5461 819.3723 2455.0951 2455.1181 -9.38 1 4 2.2 +1Score > 20 indicates identity ETALNEVMDMLRMFGLPADDR + 2 Oxidation (M)
403 414.1881 826.3617 826.3643 -3.15 0 4 2 +1Score > 19 indicates identity MYVDGAR + Oxidation (M)
2238 562.7557 1123.4968 1123.5179 -18.8 1 4 1.4 +1Score > 18 indicates identity SIQTMEDRK + Deamidated (NQ); Oxidation (M)
1052 468.2127 934.4109 934.4290 -19.4 1 4 1.9 +1Score > 19 indicates identity AEGSERMR
1167 468.2127 934.4109 934.3967 15.2 0 4 1.9 +1Score > 19 indicates identity NWEMAER
4930 778.8539 1555.6932 1555.6936 -0.28 1 4 1.6 +1Score > 18 indicates identity SFMQEREETGASGK
1925 535.7434 1069.4723 1069.4750 -2.55 1 4 1.2 +1Score > 17 indicates identity MDQFKQEK + Deamidated (NQ); Oxidation (M)
4776 765.3478 2293.0215 2292.9952 11.5 0 4 2.2 +1Score > 19 indicates identity FALMNMDGMTYEELDLSGEK
1399 495.2250 1482.6531 1482.6634 -6.93 1 4 1.9 +1Score > 19 indicates identity QTDSSKQVHHCR + Deamidated (NQ)
5966 859.8907 3435.5339 3435.5223 3.37 1 4 1.1 +1Score > 16 indicates identity QWEDFAHELCHVLKHAGNQLYMNQMFR + 2 Deamidated (NQ); 2 Oxidation (M)
6565 900.4092 1798.8038 1798.7978 3.36 1 4 1.8 +1Score > 19 indicates identity AEEAARFMNGMAEALR + Deamidated (NQ); 2 Oxidation (M)
2260 562.7557 1123.4968 1123.5145 -15.8 1 4 1.4 +1Score > 18 indicates identity RYEEVDGQK + Deamidated (NQ)
2418 576.2618 2301.0183 2301.0623 -19.1 0 4 2.2 +1Score > 20 indicates identity SLMQHGEDFLYQYNLNSLK + 2 Deamidated (NQ)
1903 535.7434 2138.9445 2138.9758 -14.6 0 4 1.2 +1Score > 17 indicates identity NAMGAANAMVAADMALAGITSR + Deamidated (NQ); 2 Oxidation (M)
5138 792.3600 1582.7055 1582.7151 -6.03 0 4 0.73 +1Score > 20 indicates identity
Score > 15 indicates homology
ADEKPPYTYADGEK
337 400.6820 1598.6989 1598.6738 15.7 0 3 0.98 +1Score > 16 indicates identity TNACMNIDMIGSVR + 2 Deamidated (NQ); Oxidation (M)
1008 454.7066 907.3986 907.4069 -9.16 1 3 1.8 +1Score > 19 indicates identity MVKENDR + Deamidated (NQ); Oxidation (M)
1438 495.2250 1482.6531 1482.6330 13.6 1 3 1.9 +1Score > 19 indicates identity MDQNQQKLTAMR + 4 Deamidated (NQ); Oxidation (M)
56 387.1759 772.3372 772.3248 16.1 0 3 1.2 +1Score > 17 indicates identity MNAYMK + Oxidation (M)
52 387.1759 1544.6744 1544.6824 -5.17 1 3 1.2 +1Score > 17 indicates identity CQHCQKNEATIR + Deamidated (NQ)
1901 535.7434 2138.9445 2138.9758 -14.6 0 3 1.2 +1Score > 17 indicates identity NAMGAANAMVAADMALAGITSR + Deamidated (NQ); 2 Oxidation (M)
2078 549.2496 1096.4846 1096.4978 -12.0 0 3 0.84 +1Score > 20 indicates identity
Score > 15 indicates homology
YYGYTGAFR
28 387.1759 772.3372 772.3248 16.1 0 3 1.2 +1Score > 17 indicates identity MNAYMK + Oxidation (M)
3919 697.8171 1393.6196 1393.5957 17.2 0 3 1.4 +1Score > 17 indicates identity SQNSADANISSDGK + Deamidated (NQ)
465 414.1881 1239.5426 1239.5368 4.70 0 3 2.1 +1Score > 19 indicates identity TDVTDFSDNAR
4067 697.8171 2787.2392 2787.2163 8.22 1 3 1.4 +1Score > 17 indicates identity HIDYNGRLCMSAAATAANQTFGMDR + Deamidated (NQ); Oxidation (M)
409 414.1881 1652.7235 1652.7273 -2.31 0 3 2.1 +1Score > 19 indicates identity SQLMQCAQLINQAK + 5 Deamidated (NQ); Oxidation (M)
3082 630.2864 2517.1164 2517.1183 -0.74 1 3 2.2 +1Score > 19 indicates identity IEKAFQHGEEWEINGDENQIK + 4 Deamidated (NQ)
5337 805.8662 1609.7179 1609.7471 -18.1 1 3 1.8 +1Score > 19 indicates identity AIYNNEVSQKGEEK + 2 Deamidated (NQ)
34 387.1759 1544.6744 1544.7003 -16.8 0 3 1.2 +1Score > 17 indicates identity GSVPFNVMMFASDK + Oxidation (M)
5682 832.8785 3327.4848 3327.4561 8.63 1 3 1.3 +1Score > 17 indicates identity GHNNVQGACDMGTLPGWLPGYQHITDDKAR + 4 Deamidated (NQ); Oxidation (M)
2440 576.2618 2301.0183 2301.0623 -19.1 0 3 2.3 +1Score > 20 indicates identity SLMQHGEDFLYQYNLNSLK + 2 Deamidated (NQ)
3763 684.3109 1366.6073 1366.6034 2.83 1 3 2.2 +1Score > 19 indicates identity MANQQSKTNAQK + 3 Deamidated (NQ); Oxidation (M)
5506 819.3723 1636.7301 1636.7515 -13.1 1 3 2.3 +1Score > 20 indicates identity QNRNLAFTPENGMK + 2 Deamidated (NQ); Oxidation (M)
104 387.1759 772.3372 772.3286 11.1 0 3 1.2 +1Score > 17 indicates identity MNHESR
1496 495.2250 1482.6531 1482.6409 8.25 0 3 2 +1Score > 19 indicates identity NDNHSPAPQMINK + 2 Deamidated (NQ); Oxidation (M)
1771 522.2372 1042.4599 1042.4753 -14.8 0 3 2.1 +1Score > 19 indicates identity MTDASLSFR + Oxidation (M)
2481 576.2618 1150.5091 1150.5176 -7.34 0 3 2.3 +1Score > 20 indicates identity GQATDMEISAK + Deamidated (NQ)
1242 481.7188 961.4231 961.4352 -12.6 1 3 1.3 +1Score > 17 indicates identity DAGDQKAEK + Deamidated (NQ)
1760 522.2372 1563.6899 1563.6987 -5.63 1 3 2.1 +1Score > 19 indicates identity MNHENREQYTLK + 2 Deamidated (NQ)
100 387.1759 772.3372 772.3351 2.67 0 3 1.2 +1Score > 17 indicates identity DPDQAAR + Deamidated (NQ)
1639 508.7311 2030.8955 2030.8626 16.2 0 3 1.1 +1Score > 16 indicates identity ASELETAFQTASSNANQMK + 4 Deamidated (NQ)
2431 576.2618 1150.5091 1150.5254 -14.2 0 3 2.3 +1Score > 20 indicates identity AYLAAADGDER
2539 576.2618 1725.7637 1725.7879 -14.1 0 3 2.3 +1Score > 20 indicates identity GELQCIGATTLDEYR + Deamidated (NQ)
3889 684.3109 2733.2145 2733.2399 -9.26 1 3 2.2 +1Score > 19 indicates identity CQNSKLEAAVTQAEQQGEVALNDAR + 4 Deamidated (NQ)
463 414.1881 826.3617 826.3709 -11.0 0 3 2.1 +1Score > 19 indicates identity TYQTEGK + Deamidated (NQ)
761 441.2004 1320.5795 1320.6045 -18.9 0 3 2.2 +1Score > 19 indicates identity TDAADETVSGIDK
1072 468.2127 934.4109 934.4290 -19.4 1 3 2 +1Score > 19 indicates identity AEGSERMR
2416 576.2618 1725.7637 1725.7590 2.75 0 3 2.3 +1Score > 20 indicates identity MSFDPNNVLMQDAVK + 2 Deamidated (NQ); Oxidation (M)
154 387.1759 1544.6744 1544.6824 -5.17 1 3 1.2 +1Score > 17 indicates identity CQHCQKNEATIR + Deamidated (NQ)
3170 630.2864 1887.8373 1887.8374 -0.044 0 3 2.2 +1Score > 19 indicates identity SGQLQYEETITEGFER + 2 Deamidated (NQ)
577 427.6943 853.3741 853.3786 -5.31 1 3 1 +1Score > 16 indicates identity QMMKQR + Deamidated (NQ); 2 Oxidation (M)
2075 549.2496 1096.4846 1096.4754 8.42 1 3 0.95 +1Score > 20 indicates identity
Score > 16 indicates homology
CMRSLDGSR + Oxidation (M)
5961 859.8907 3435.5339 3435.5223 3.37 1 3 1.1 +1Score > 16 indicates identity QWEDFAHELCHVLKHAGNQLYMNQMFR + 2 Deamidated (NQ); 2 Oxidation (M)
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