MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 7572)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3591 670.8048 1339.5951
3592 670.8048 1339.5951
3593 670.8048 1339.5951
3594 670.8048 1339.5951
3595 670.8048 1339.5951
3597 670.8048 1339.5951
3599 670.8048 1339.5951
3600 670.8048 1339.5951
3601 670.8048 1339.5951
3602 670.8048 1339.5951
3603 670.8048 1339.5951
3604 670.8048 1339.5951
3605 670.8048 1339.5951
3607 670.8048 1339.5951
3608 670.8048 1339.5951
3611 670.8048 1339.5951
3612 670.8048 1339.5951
3613 670.8048 1339.5951
3614 670.8048 1339.5951
3615 670.8048 1339.5951
3616 670.8048 1339.5951
3618 670.8048 1339.5951
3619 670.8048 1339.5951
3620 670.8048 1339.5951
3621 670.8048 1339.5951
3622 670.8048 1339.5951
3623 670.8048 1339.5951
3624 670.8048 1339.5951
3625 670.8048 1339.5951
3626 670.8048 1339.5951
3627 670.8048 1339.5951
3628 670.8048 1339.5951
3629 670.8048 1339.5951
3630 670.8048 1339.5951
3631 670.8048 1339.5951
3632 670.8048 1339.5951
3634 670.8048 1339.5951
3635 670.8048 1339.5951
3637 670.8048 1339.5951
3638 670.8048 1339.5951
3639 670.8048 1339.5951
3640 670.8048 1339.5951
3641 670.8048 1339.5951
3642 670.8048 1339.5951
3643 670.8048 1339.5951
3644 670.8048 1339.5951
3647 670.8048 1339.5951
3648 670.8048 1339.5951
3649 670.8048 1339.5951
3650 670.8048 1339.5951
3651 670.8048 1339.5951
3652 670.8048 1339.5951
3653 670.8048 1339.5951
3654 670.8048 1339.5951
3655 670.8048 1339.5951
3656 670.8048 1339.5951
3657 670.8048 1339.5951
3658 670.8048 1339.5951
3659 670.8048 1339.5951
3660 670.8048 1339.5951
3662 670.8048 1339.5951
3663 670.8048 1339.5951
3665 670.8048 1339.5951
3666 670.8048 1339.5951
3667 670.8048 1339.5951
3668 670.8048 1339.5951
3670 670.8048 1339.5951
3671 670.8048 1339.5951
3672 670.8048 1339.5951
3673 670.8048 1339.5951
3674 670.8048 1339.5951
3675 670.8048 1339.5951
3676 670.8048 1339.5951
3677 670.8048 1339.5951
3678 670.8048 1339.5951
3679 670.8048 1339.5951
3680 670.8048 1339.5951
3681 670.8048 1339.5951
3682 670.8048 1339.5951
3683 670.8048 1339.5951
3684 670.8048 1339.5951
3685 670.8048 1339.5951
3686 670.8048 1339.5951
3687 670.8048 2679.1901 2679.1983 -3.04 1     +0Score > 18 indicates identity AIQNGGHEIGNHSYNHPDMSKLTR + 4 Deamidated (NQ)
3689 670.8048 1339.5951
3690 670.8048 1339.5951
3691 670.8048 1339.5951
3693 670.8048 1339.5951
3694 670.8048 1339.5951
3695 670.8048 1339.5951
3697 670.8048 1339.5951
3699 670.8048 1339.5951
3702 670.8048 1339.5951
3703 670.8048 1339.5951
3704 670.8048 1339.5951
3705 670.8048 1339.5951
3706 670.8048 1339.5951
3707 670.8048 1339.5951
3708 670.8048 1339.5951
3709 670.8048 1339.5951
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