| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | test |
| MS data file | : | PRT1346_ENIGMA_14.mgf |
| Database | : | B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues) |
| Timestamp | : | 4 Dec 2025 at 15:32:34 GMT |
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the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 7,621 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 873.3969 | 2617.1689 | 2617.1425 | 10.1 | 1 | 6 | 1.2 | 1Score > 19 indicates identity |
DCGSDQFPDLSGRECYVGIDLSK | |
| 832.8785 | 1663.7424 | 1663.7246 | 10.7 | 0 | 6 | 0.76 | 1Score > 17 indicates identity |
MNDEEAQIIANNGVK + 3 Deamidated (NQ); Oxidation (M) | |
| 886.9030 | 1771.7915 | 1771.8120 | -11.6 | 1 | 6 | 0.8 | 1Score > 17 indicates identity |
NNFNNMKNVVTTVMK + 3 Deamidated (NQ); Oxidation (M) | |
| 481.7188 | 961.4231 | 961.4352 | -12.6 | 0 | 6 | 0.77 | 1Score > 17 indicates identity |
LDQEQDSK | |
| 630.2864 | 1258.5582 | 1258.5573 | 0.68 | 0 | 6 | 1.3 | 1Score > 19 indicates identity |
IMDAAGYEVMK + 2 Oxidation (M) | |
| 427.6943 | 1706.7481 | 1706.7649 | -9.84 | 0 | 6 | 0.57 | 1Score > 16 indicates identity |
GSLGGGFSSGGFSGGSFSR | |
| 819.3723 | 2455.0951 | 2455.1391 | -17.9 | 0 | 6 | 1.3 | 1Score > 20 indicates identity |
FLYGTQGQGGEGQPQTQTALITR + 5 Deamidated (NQ) | |
| 846.3846 | 1690.7546 | 1690.7686 | -8.25 | 1 | 6 | 1.1 | 1Score > 19 indicates identity |
QDVESYQKNEQPPK + 2 Deamidated (NQ) | |
| 495.2250 | 1976.8708 | 1976.8633 | 3.80 | 0 | 6 | 1.2 | 1Score > 19 indicates identity |
QMNGEDITQEEVEQAQK + Deamidated (NQ) | |
| 765.3478 | 2293.0215 | 2293.0184 | 1.37 | 0 | 6 | 1.3 | 1Score > 19 indicates identity |
SMMYEGLQHYFIVNFPQR + 2 Deamidated (NQ); 2 Oxidation (M) | |
| 508.7311 | 2030.8955 | 2030.8626 | 16.2 | 0 | 6 | 0.63 | 1Score > 16 indicates identity |
ASELETAFQTASSNANQMK + 4 Deamidated (NQ) | |
| 589.7679 | 1177.5213 | 1177.5099 | 9.75 | 1 | 6 | 0.8 | 1Score > 17 indicates identity |
GLADDDSDNKK + Deamidated (NQ) | |
| 589.7679 | 2355.0427 | 2355.0835 | -17.3 | 0 | 6 | 0.8 | 1Score > 17 indicates identity |
AMDAEAGVMISASHNPVQDNGIK + Deamidated (NQ) | |
| 765.3478 | 3057.3620 | 3057.3194 | 13.9 | 0 | 6 | 1.3 | 1Score > 19 indicates identity |
MNTEQTIEVMNQNWTDIYYHLHMK + 3 Deamidated (NQ); Oxidation (M) | |
| 630.2864 | 2517.1164 | 2517.1516 | -14.0 | 1 | 6 | 1.3 | 1Score > 19 indicates identity |
SGRQGDPGITQFYLSMEDELMK + Oxidation (M) | |
| 589.7679 | 1177.5213 | 1177.5211 | 0.22 | 0 | 6 | 0.8 | 1Score > 17 indicates identity |
GTSQINASQNR + 3 Deamidated (NQ) | |
| 630.2864 | 1258.5582 | 1258.5499 | 6.58 | 1 | 6 | 1.3 | 1Score > 19 indicates identity |
HKDMNDLEEK + Deamidated (NQ) | |
| 792.3600 | 1582.7055 | 1582.7045 | 0.64 | 1 | 6 | 0.83 | 1Score > 20 indicates identityScore > 17 indicates homology |
CQYETLVENNRR + 2 Deamidated (NQ) | |
| 846.3846 | 1690.7546 | 1690.7686 | -8.25 | 1 | 6 | 1.1 | 1Score > 19 indicates identity |
QDVESYQKNEQPPK + 2 Deamidated (NQ) | |
| 873.3969 | 2617.1689 | 2617.2193 | -19.2 | 0 | 6 | 1.2 | 1Score > 19 indicates identity |
LYEMFMGPLDASIAWSTTGLDGAR + Oxidation (M) | |
| 562.7557 | 2246.9936 | 2247.0114 | -7.91 | 1 | 6 | 0.89 | 1Score > 18 indicates identity |
SKDEFGQLGTSFNEMADSLR + Oxidation (M) | |
| 927.4214 | 1852.8283 | 1852.8512 | -12.4 | 0 | 6 | 0.72 | 1Score > 20 indicates identityScore > 17 indicates homology |
TEMLTNNIANANTPGYK + 2 Deamidated (NQ) | |
| 589.7679 | 1177.5213 | 1177.5099 | 9.74 | 1 | 6 | 0.82 | 1Score > 17 indicates identity |
VDADSGEDDKK | |
| 913.9153 | 1825.8160 | 1825.8516 | -19.5 | 1 | 6 | 1.1 | 1Score > 18 indicates identity |
MVDQYVRNLNSDLNK + 2 Deamidated (NQ); Oxidation (M) | |
| 576.2618 | 1150.5091 | 1150.5255 | -14.2 | 0 | 6 | 1.4 | 1Score > 20 indicates identity |
FSQLNQNDGK + Deamidated (NQ) | |
| 414.1881 | 1239.5426 | 1239.5554 | -10.3 | 1 | 6 | 1.3 | 1Score > 19 indicates identity |
FQAQEREMGK + Deamidated (NQ); Oxidation (M) | |
| 481.7188 | 961.4231 | 961.4352 | -12.6 | 1 | 6 | 0.81 | 1Score > 17 indicates identity |
DAGDQKAEK + Deamidated (NQ) | |
| 535.7434 | 2138.9445 | 2138.9758 | -14.6 | 0 | 6 | 0.76 | 1Score > 17 indicates identity |
NAMGAANAMVAADMALAGITSR + Deamidated (NQ); 2 Oxidation (M) | |
| 535.7434 | 2138.9445 | 2138.9711 | -12.4 | 1 | 6 | 0.76 | 1Score > 17 indicates identity |
AGINTVQELANKTEEDMMK + 2 Deamidated (NQ); Oxidation (M) | |
| 400.6820 | 799.3495 | 799.3646 | -19.0 | 0 | 5 | 0.62 | 1Score > 16 indicates identity |
MEHINR + Deamidated (NQ) | |
| 576.2618 | 1725.7637 | 1725.7853 | -12.5 | 1 | 5 | 1.4 | 1Score > 20 indicates identity |
REQGHDIDAACGQLR + Deamidated (NQ) | |
| 387.1759 | 772.3372 | 772.3248 | 16.1 | 0 | 5 | 0.74 | 1Score > 17 indicates identity |
MNAYMK + Oxidation (M) | |
| 414.1881 | 1239.5426 | 1239.5628 | -16.3 | 0 | 5 | 1.3 | 1Score > 19 indicates identity |
IGQMLMPDFR + Deamidated (NQ); 2 Oxidation (M) | |
| 495.2250 | 988.4354 | 988.4219 | 13.7 | 0 | 5 | 1.2 | 1Score > 19 indicates identity |
GNHEVMMR + Oxidation (M) | |
| 468.2127 | 1868.8217 | 1868.8437 | -11.7 | 1 | 5 | 1.2 | 1Score > 19 indicates identity |
MIQKMAEYQSYTGFR + Deamidated (NQ); Oxidation (M) | |
| 387.1759 | 1158.5058 | 1158.4975 | 7.15 | 0 | 5 | 0.75 | 1Score > 17 indicates identity |
EHLQTCNEK + Deamidated (NQ) | |
| 630.2864 | 1258.5582 | 1258.5798 | -17.2 | 0 | 5 | 1.4 | 1Score > 19 indicates identity |
MVHMIDVNER + Oxidation (M) | |
| 643.7925 | 1285.5705 | 1285.5907 | -15.7 | 1 | 5 | 0.92 | 1Score > 18 indicates identity |
RLTGMFANGMR + Deamidated (NQ); 2 Oxidation (M) | |
| 765.3478 | 2293.0215 | 2293.0507 | -12.7 | 0 | 5 | 1.4 | 1Score > 19 indicates identity |
MYHLYTHNDLDGVGCGILAK + Deamidated (NQ); Oxidation (M) | |
| 913.9153 | 3651.6320 | 3651.6991 | -18.4 | 1 | 5 | 1.1 | 1Score > 18 indicates identity |
EFDIITTGDYQLILEDDQELYAYLRNGADEK + 2 Deamidated (NQ) | |
| 508.7311 | 1015.4477 | 1015.4323 | 15.2 | 1 | 5 | 0.69 | 1Score > 16 indicates identity |
MMKMMAGGK + 2 Oxidation (M) | |
| 481.7188 | 961.4231 | 961.4101 | 13.6 | 0 | 5 | 0.85 | 1Score > 17 indicates identity |
NGATNVNNR + 3 Deamidated (NQ) | |
| 900.4092 | 1798.8038 | 1798.8264 | -12.5 | 1 | 5 | 1.2 | 1Score > 19 indicates identity |
SGMSPCSGVIVGMKETK + 2 Oxidation (M) | |
| 400.6820 | 799.3495 | 799.3422 | 9.09 | 0 | 5 | 0.65 | 1Score > 16 indicates identity |
FTMEEK + Oxidation (M) | |
| 495.2250 | 1482.6531 | 1482.6409 | 8.25 | 0 | 5 | 1.3 | 1Score > 19 indicates identity |
NDNHSPAPQMINK + 2 Deamidated (NQ); Oxidation (M) | |
| 495.2250 | 988.4354 | 988.4219 | 13.7 | 0 | 5 | 1.3 | 1Score > 19 indicates identity |
GNHEVMMR + Oxidation (M) | |
| 603.2741 | 1204.5337 | 1204.5394 | -4.75 | 0 | 5 | 1.3 | 1Score > 19 indicates identity |
MSGAETIHNTK + Deamidated (NQ); Oxidation (M) | |
| 792.3600 | 3165.4111 | 3165.3704 | 12.8 | 1 | 5 | 1 | 1Score > 20 indicates identityScore > 18 indicates homology |
WNGLADLKLMNMMGYDAMTFGNHEFDK + Deamidated (NQ); Oxidation (M) | |
| 576.2618 | 1150.5091 | 1150.5295 | -17.7 | 0 | 5 | 1.5 | 1Score > 20 indicates identity |
LGYFHEEEK | |
| 846.3846 | 3381.5092 | 3381.4581 | 15.1 | 0 | 5 | 1.2 | 1Score > 19 indicates identity |
SLLDDGSFEEFNQDMMSENPLEFPGYLEK + Deamidated (NQ) | |
| 576.2618 | 2301.0183 | 2301.0252 | -3.03 | 1 | 5 | 1.5 | 1Score > 20 indicates identity |
NAKTQSLACVASYEAMANNEK + 2 Deamidated (NQ) | |
| 711.3232 | 1420.6319 | 1420.6218 | 7.10 | 1 | 5 | 1.4 | 1Score > 19 indicates identity |
EPENQQAFRGSR + 3 Deamidated (NQ) | |
| 454.7066 | 907.3986 | 907.3957 | 3.22 | 0 | 5 | 1.2 | 1Score > 19 indicates identity |
MDQQQLK + 2 Deamidated (NQ); Oxidation (M) | |
| 535.7434 | 1069.4723 | 1069.4750 | -2.55 | 1 | 5 | 0.81 | 1Score > 17 indicates identity |
MDQFKQEK + Deamidated (NQ); Oxidation (M) | |
| 576.2618 | 1725.7637 | 1725.7662 | -1.43 | 1 | 5 | 1.5 | 1Score > 20 indicates identity |
SDNLKSQMQDIMSGR + Deamidated (NQ); Oxidation (M) | |
| 711.3232 | 2130.9479 | 2130.9384 | 4.47 | 1 | 5 | 1.4 | 1Score > 19 indicates identity |
QDMACLIREYQEVMNSK + Deamidated (NQ); Oxidation (M) | |
| 711.3232 | 1420.6319 | 1420.6504 | -13.0 | 0 | 5 | 1.4 | 1Score > 19 indicates identity |
DAMDGLSLANQLR + 2 Deamidated (NQ); Oxidation (M) | |
| 481.7188 | 961.4231 | 961.4352 | -12.6 | 1 | 5 | 0.87 | 1Score > 17 indicates identity |
QEKQADDK + Deamidated (NQ) | |
| 427.6943 | 1706.7481 | 1706.7649 | -9.84 | 0 | 5 | 0.65 | 1Score > 16 indicates identity |
GSLGGGFSSGGFSGGSFSR | |
| 387.1759 | 1544.6744 | 1544.7028 | -18.4 | 0 | 5 | 0.79 | 1Score > 17 indicates identity |
TQEMLEHELEASK + Deamidated (NQ) | |
| 576.2618 | 1150.5091 | 1150.5077 | 1.26 | 1 | 5 | 1.5 | 1Score > 20 indicates identity |
WKNMTADER + Deamidated (NQ) | |
| 441.2004 | 1320.5795 | 1320.6044 | -18.9 | 1 | 5 | 1.5 | 1Score > 19 indicates identity |
LSTKNQENEEK + 2 Deamidated (NQ) | |
| 792.3600 | 1582.7055 | 1582.7045 | 0.64 | 1 | 5 | 0.47 | 1Score > 20 indicates identityScore > 14 indicates homology |
CQYETLVENNRR + 2 Deamidated (NQ) | |
| 697.8171 | 1393.6196 | 1393.6184 | 0.89 | 0 | 5 | 0.97 | 1Score > 17 indicates identity |
MNGGWETIVNQK + 2 Deamidated (NQ); Oxidation (M) | |
| 522.2372 | 2084.9199 | 2084.9361 | -7.77 | 0 | 5 | 1.4 | 1Score > 19 indicates identity |
SDNCEDTPEAGYFAIAVVK | |
| 549.2496 | 1096.4846 | 1096.4754 | 8.42 | 1 | 5 | 0.99 | 1Score > 20 indicates identityScore > 18 indicates homology |
CMRSLDGSR + Oxidation (M) | |
| 549.2496 | 2192.9692 | 2192.9644 | 2.18 | 0 | 5 | 1 | 1Score > 20 indicates identityScore > 18 indicates homology |
TTQANEIMINSETFDHADR + Deamidated (NQ) | |
| 819.3723 | 3273.4602 | 3273.5163 | -17.1 | 1 | 5 | 1.6 | 1Score > 20 indicates identity |
MTTISNAMSRQVATISSNQTVQEAAELMSR + 3 Deamidated (NQ); Oxidation (M) | |
| 495.2250 | 1976.8708 | 1976.8633 | 3.80 | 0 | 5 | 1.4 | 1Score > 19 indicates identity |
QMNGEDITQEEVEQAQK + Deamidated (NQ) | |
| 576.2618 | 1725.7637 | 1725.7781 | -8.34 | 0 | 5 | 1.6 | 1Score > 20 indicates identity |
VHSECLTGDVFGSYR | |
| 495.2250 | 988.4354 | 988.4219 | 13.7 | 0 | 5 | 1.4 | 1Score > 19 indicates identity |
GNHEVMMR + Oxidation (M) | |
| 508.7311 | 2030.8955 | 2030.8626 | 16.2 | 1 | 5 | 0.74 | 1Score > 16 indicates identity |
NDTKMTYEELEEQLNR + 3 Deamidated (NQ); Oxidation (M) | |
| 603.2741 | 1806.8005 | 1806.7838 | 9.24 | 1 | 5 | 1.4 | 1Score > 19 indicates identity |
AMGMVEMKQGEGTYVK + Deamidated (NQ); 3 Oxidation (M) | |
| 603.2741 | 1204.5337 | 1204.5571 | -19.5 | 0 | 5 | 1.4 | 1Score > 19 indicates identity |
STAEAENVDIR + Deamidated (NQ) | |
| 387.1759 | 772.3372 | 772.3248 | 16.1 | 0 | 5 | 0.82 | 1Score > 17 indicates identity |
MNAYMK + Oxidation (M) | |
| 913.9153 | 3651.6320 | 3651.6991 | -18.4 | 1 | 5 | 1.2 | 1Score > 18 indicates identity |
EFDIITTGDYQLILEDDQELYAYLRNGADEK + 2 Deamidated (NQ) | |
| 441.2004 | 1320.5795 | 1320.6020 | -17.0 | 0 | 5 | 1.5 | 1Score > 19 indicates identity |
GQILFMEDDPR + Deamidated (NQ) | |
| 670.8048 | 1339.5951 | 1339.6190 | -17.9 | 1 | 5 | 1.2 | 1Score > 18 indicates identity |
KDSGFQMNQLR + Deamidated (NQ); Oxidation (M) | |
| 562.7557 | 1123.4968 | 1123.5002 | -3.00 | 0 | 5 | 1 | 1Score > 18 indicates identity |
GMGIIGMEER + 2 Oxidation (M) | |
| 589.7679 | 1177.5213 | 1177.5211 | 0.22 | 0 | 5 | 0.94 | 1Score > 17 indicates identity |
GTSQINASQNR + 3 Deamidated (NQ) | |
| 576.2618 | 1150.5091 | 1150.5063 | 2.45 | 0 | 5 | 1.6 | 1Score > 20 indicates identity |
MNQIQLEEK + 3 Deamidated (NQ); Oxidation (M) | |
| 738.3355 | 2211.9847 | 2212.0028 | -8.16 | 1 | 5 | 0.58 | 1Score > 20 indicates identityScore > 15 indicates homology |
MDNQELTMPTKYDPSAVEK + Oxidation (M) | |
| 873.3969 | 1744.7793 | 1744.7726 | 3.80 | 0 | 5 | 1.5 | 1Score > 19 indicates identity |
EDGENHWMVEGLVSK + Oxidation (M) | |
| 940.9276 | 3759.6811 | 3759.7174 | -9.64 | 1 | 5 | 0.9 | 1Score > 17 indicates identity |
KELIEEMGDVMGNLIMLAGIYGVSLEDMMDAHR + 5 Oxidation (M) | |
| 630.2864 | 1258.5582 | 1258.5798 | -17.2 | 0 | 5 | 1.6 | 1Score > 19 indicates identity |
MVHMIDVNER + Oxidation (M) | |
| 805.8662 | 3219.4357 | 3219.3819 | 16.7 | 1 | 5 | 1.3 | 1Score > 19 indicates identity |
AGNSAMANNQFTIGKNLNTVESDISSMNNR + 6 Deamidated (NQ); Oxidation (M) | |
| 697.8171 | 1393.6196 | 1393.6473 | -19.9 | 0 | 5 | 1 | 1Score > 17 indicates identity |
QSTQEELANAFR + Deamidated (NQ) | |
| 441.2004 | 1320.5795 | 1320.6058 | -19.9 | 1 | 5 | 1.6 | 1Score > 19 indicates identity |
DNNRVGFAEAAR + 2 Deamidated (NQ) | |
| 832.8785 | 1663.7424 | 1663.7624 | -12.0 | 1 | 5 | 0.93 | 1Score > 17 indicates identity |
NRMNGGWETIVNQK + 2 Deamidated (NQ); Oxidation (M) | |
| 400.6820 | 1598.6989 | 1598.7286 | -18.6 | 0 | 5 | 0.73 | 1Score > 16 indicates identity |
DMFNLQANAFDIAK + 2 Deamidated (NQ) | |
| 832.8785 | 1663.7424 | 1663.7480 | -3.34 | 1 | 5 | 0.94 | 1Score > 17 indicates identity |
TARNMNPLMAMAADR + 2 Deamidated (NQ) | |
| 468.2127 | 1868.8217 | 1868.8462 | -13.1 | 0 | 5 | 1.4 | 1Score > 19 indicates identity |
TEMLTNNIANANTPGYK + 2 Deamidated (NQ); Oxidation (M) | |
| 724.8293 | 1447.6441 | 1447.6215 | 15.6 | 0 | 5 | 0.99 | 1Score > 17 indicates identity |
QWSAEDEEAVQR + Deamidated (NQ) | |
| 792.3600 | 3165.4111 | 3165.3704 | 12.8 | 1 | 5 | 1 | 1Score > 20 indicates identityScore > 17 indicates homology |
WNGLADLKLMNMMGYDAMTFGNHEFDK + Deamidated (NQ); Oxidation (M) | |
| 873.3969 | 1744.7793 | 1744.7865 | -4.17 | 0 | 5 | 1.5 | 1Score > 19 indicates identity |
EFSDEMNVFLQQLK + 2 Deamidated (NQ); Oxidation (M) | |
| 711.3232 | 2130.9479 | 2130.9635 | -7.33 | 1 | 5 | 1.6 | 1Score > 19 indicates identity |
MDNQVLQAIYEMQKEMK + Deamidated (NQ); 2 Oxidation (M) | |
| 387.1759 | 1544.6744 | 1544.6673 | 4.60 | 0 | 5 | 0.87 | 1Score > 17 indicates identity |
EQMMYDMEALLR + Oxidation (M) | |
| 414.1881 | 1239.5426 | 1239.5554 | -10.3 | 1 | 5 | 1.5 | 1Score > 19 indicates identity |
FQAQEREMGK + Deamidated (NQ); Oxidation (M) | |
| 522.2372 | 1042.4599 | 1042.4753 | -14.8 | 0 | 5 | 1.6 | 1Score > 19 indicates identity |
MTDASLSFR + Oxidation (M) | |
| 427.6943 | 853.3741 | 853.3786 | -5.31 | 1 | 5 | 0.72 | 1Score > 16 indicates identity |
QMMKQR + Deamidated (NQ); 2 Oxidation (M) |
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the select summary.