MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 7572)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3241 643.7925 1285.5705
3242 643.7925 1285.5705
3244 643.7925 1285.5705
3245 643.7925 1285.5705
3246 643.7925 1285.5705
3247 643.7925 1285.5705
3248 643.7925 1285.5705
3249 643.7925 1285.5705
3251 643.7925 1285.5705
3252 643.7925 1285.5705
3253 643.7925 1285.5705
3254 643.7925 1285.5705
3255 643.7925 1285.5705
3257 643.7925 1285.5705
3258 643.7925 1285.5705
3259 643.7925 1285.5705
3260 643.7925 1285.5705
3261 643.7925 1285.5705
3262 643.7925 1285.5705
3266 643.7925 1285.5705
3267 643.7925 1285.5705
3269 643.7925 1285.5705
3271 643.7925 1285.5705
3272 643.7925 1285.5705
3273 643.7925 1285.5705
3274 643.7925 1285.5705
3275 643.7925 1285.5705
3277 643.7925 1285.5705
3278 643.7925 1285.5705
3279 643.7925 1285.5705
3280 643.7925 1285.5705
3281 643.7925 1285.5705
3282 643.7925 1285.5705
3283 643.7925 1285.5705
3284 643.7925 1285.5705
3286 643.7925 1285.5705
3287 643.7925 1285.5705
3288 643.7925 1285.5705
3289 643.7925 1285.5705
3291 643.7925 1285.5705
3292 643.7925 1285.5705
3293 643.7925 1285.5705
3294 643.7925 1285.5705
3295 643.7925 1285.5705
3296 643.7925 1285.5705
3297 643.7925 1285.5705
3298 643.7925 1285.5705
3299 643.7925 1285.5705
3300 643.7925 1285.5705
3301 643.7925 1285.5705
3302 643.7925 1285.5705
3303 643.7925 1285.5705
3304 643.7925 1285.5705
3305 643.7925 1285.5705
3306 643.7925 1285.5705
3307 643.7925 1285.5705
3308 643.7925 1285.5705
3309 643.7925 1285.5705
3310 643.7925 1285.5705
3311 643.7925 1285.5705
3312 643.7925 1285.5705
3313 643.7925 1285.5705
3314 643.7925 1285.5705
3315 643.7925 1285.5705
3316 643.7925 1285.5705
3317 643.7925 1285.5705
3318 643.7925 1285.5705
3319 643.7925 1285.5705
3320 643.7925 1285.5705
3321 643.7925 1285.5705
3322 643.7925 1285.5705
3323 643.7925 1285.5705
3324 643.7925 1285.5705
3325 643.7925 1285.5705
3326 643.7925 1285.5705
3327 643.7925 1285.5705
3328 643.7925 1285.5705
3329 643.7925 1285.5705
3330 643.7925 1285.5705
3331 643.7925 1285.5705
3332 643.7925 1285.5705
3333 643.7925 1285.5705
3334 643.7925 1285.5705
3335 643.7925 1285.5705
3337 643.7925 1285.5705
3338 643.7925 1285.5705
3339 643.7925 1285.5705
3341 643.7925 1285.5705
3342 643.7925 1285.5705
3343 643.7925 1285.5705
3344 643.7925 1285.5705
3345 643.7925 1285.5705
3346 643.7925 1285.5705
3347 643.7925 1285.5705
3348 643.7925 1285.5705
3349 643.7925 1285.5705
3350 643.7925 1285.5705
3351 643.7925 1285.5705
3353 643.7925 1285.5705
3354 643.7925 1285.5705
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