MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 7572)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2993 616.7802 1231.5459
2994 616.7802 1231.5459
2996 616.7802 1231.5459
2997 616.7802 1231.5459
2998 616.7802 1231.5459
2999 616.7802 1231.5459
3000 616.7802 1231.5459
3001 616.7802 1231.5459
3002 616.7802 1231.5459
3003 616.7802 1231.5459
3005 616.7802 1231.5459
3006 616.7802 1231.5459
3007 616.7802 1231.5459
3008 616.7802 1231.5459
3009 616.7802 1231.5459
3010 616.7802 1231.5459
3011 616.7802 1231.5459
3012 616.7802 1231.5459
3013 616.7802 1231.5459
3014 616.7802 1231.5459
3015 616.7802 1231.5459
3016 616.7802 1231.5459
3017 616.7802 1231.5459
3018 616.7802 1231.5459
3019 616.7802 1231.5459
3021 616.7802 1231.5459
3023 616.7802 1231.5459
3024 616.7802 1231.5459
3025 616.7802 1231.5459
3026 616.7802 1231.5459
3027 616.7802 1231.5459
3028 616.7802 1231.5459
3029 616.7802 1231.5459
3031 616.7802 1231.5459
3032 616.7802 1231.5459
3033 616.7802 1231.5459
3034 616.7802 1231.5459
3035 616.7802 1231.5459
3036 616.7802 1231.5459
3037 616.7802 1231.5459
3039 616.7802 1231.5459
3041 616.7802 1231.5459
3042 616.7802 1231.5459
3043 616.7802 1231.5459
3044 616.7802 1231.5459
3045 616.7802 1231.5459
3046 616.7802 2463.0917 2463.1046 -5.21 1     +0Score > 17 indicates identity SSAMEALQLVKHHECETGYQK + 2 Deamidated (NQ); Oxidation (M)
3047 616.7802 1231.5459
3048 616.7802 1231.5459
3049 616.7802 1231.5459
3050 616.7802 1231.5459
3051 616.7802 1231.5459
3052 616.7802 1231.5459
3053 616.7802 1231.5459
3054 616.7802 1231.5459
3055 616.7802 1231.5459
3056 616.7802 1231.5459
3057 616.7802 1231.5459
3058 616.7802 1231.5459
3059 630.2864 1258.5582
3060 630.2864 1258.5582
3061 630.2864 1258.5582
3062 630.2864 1258.5582
3063 630.2864 1258.5582
3064 630.2864 1258.5582
3065 630.2864 1258.5582
3066 630.2864 1258.5582
3067 630.2864 1258.5582
3068 630.2864 1258.5582
3069 630.2864 1258.5582
3070 630.2864 1258.5582
3071 630.2864 1258.5582
3072 630.2864 1258.5582
3073 630.2864 1258.5582
3074 630.2864 1258.5582
3075 630.2864 1258.5582
3076 630.2864 1258.5582
3077 630.2864 1258.5582
3079 630.2864 1258.5582
3081 630.2864 1258.5582
3083 630.2864 1258.5582
3084 630.2864 1258.5582
3085 630.2864 1258.5582
3089 630.2864 1258.5582
3090 630.2864 1258.5582
3092 630.2864 1258.5582
3093 630.2864 1258.5582
3096 630.2864 1258.5582
3098 630.2864 1258.5582
3099 630.2864 1258.5582
3100 630.2864 1258.5582
3101 630.2864 1258.5582
3102 630.2864 1258.5582
3106 630.2864 1258.5582
3108 630.2864 1258.5582
3109 630.2864 1258.5582
3110 630.2864 1258.5582
3111 630.2864 1258.5582
3112 630.2864 1258.5582
3113 630.2864 1258.5582
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