MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 7572)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2190 549.2496 1096.4846
2192 549.2496 1096.4846
2193 549.2496 1096.4846
2194 549.2496 1096.4846
2195 549.2496 1096.4846
2196 549.2496 1096.4846
2199 549.2496 1096.4846
2200 549.2496 1096.4846
2202 549.2496 1096.4846
2203 549.2496 1096.4846
2205 549.2496 1096.4846
2206 549.2496 1096.4846
2207 549.2496 1096.4846
2211 562.7557 1123.4968
2212 562.7557 1123.4968
2213 562.7557 1123.4968
2214 562.7557 1123.4968
2215 562.7557 1123.4968
2216 562.7557 1123.4968
2217 562.7557 1123.4968
2218 562.7557 1123.4968
2219 562.7557 1123.4968
2220 562.7557 1123.4968
2221 562.7557 1123.4968
2222 562.7557 1123.4968
2223 562.7557 1123.4968
2224 562.7557 1123.4968
2226 562.7557 1123.4968
2227 562.7557 1123.4968
2228 562.7557 1123.4968
2229 562.7557 1123.4968
2230 562.7557 1123.4968
2232 562.7557 1123.4968
2233 562.7557 1123.4968
2235 562.7557 1123.4968
2236 562.7557 1123.4968
2239 562.7557 1123.4968
2240 562.7557 1123.4968
2241 562.7557 1123.4968
2242 562.7557 1123.4968
2244 562.7557 1123.4968
2249 562.7557 1123.4968
2250 562.7557 1123.4968
2251 562.7557 1123.4968
2252 562.7557 1123.4968
2254 562.7557 1123.4968
2255 562.7557 1123.4968
2258 562.7557 1123.4968
2261 562.7557 1123.4968
2262 562.7557 1123.4968
2264 562.7557 1123.4968
2265 562.7557 1123.4968
2266 562.7557 1123.4968
2267 562.7557 1123.4968
2268 562.7557 1123.4968
2269 562.7557 1123.4968
2273 562.7557 1123.4968
2274 562.7557 1123.4968
2276 562.7557 1123.4968
2278 562.7557 1123.4968
2279 562.7557 1123.4968
2281 562.7557 1123.4968
2282 562.7557 1123.4968
2283 562.7557 1123.4968
2284 562.7557 1123.4968
2285 562.7557 1123.4968
2286 562.7557 1123.4968
2287 562.7557 1123.4968
2288 562.7557 1123.4968
2289 562.7557 1123.4968
2290 562.7557 1123.4968
2292 562.7557 1123.4968
2293 562.7557 1123.4968
2294 562.7557 1123.4968
2295 562.7557 1123.4968
2297 562.7557 1123.4968
2298 562.7557 1123.4968
2299 562.7557 1123.4968
2300 562.7557 1123.4968
2302 562.7557 1123.4968
2304 562.7557 1123.4968
2305 562.7557 1123.4968
2306 562.7557 1123.4968
2307 562.7557 1123.4968
2309 562.7557 1123.4968
2311 562.7557 1123.4968
2312 562.7557 1123.4968
2313 562.7557 1123.4968
2315 562.7557 1123.4968
2317 562.7557 1123.4968
2318 562.7557 1123.4968
2319 562.7557 1123.4968
2321 562.7557 1123.4968
2322 562.7557 1123.4968
2323 562.7557 1123.4968
2324 562.7557 1123.4968
2325 562.7557 1123.4968
2326 562.7557 1123.4968
2327 562.7557 1123.4968
2328 562.7557 1123.4968
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