MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 7572)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2031 535.7434 1069.4723
2032 535.7434 1069.4723
2033 535.7434 1069.4723
2034 535.7434 1069.4723
2036 535.7434 1069.4723
2037 535.7434 1069.4723
2038 535.7434 1069.4723
2039 535.7434 1069.4723
2040 535.7434 1069.4723
2041 549.2496 1096.4846
2042 549.2496 1096.4846
2043 549.2496 1096.4846
2044 549.2496 1096.4846
2045 549.2496 1096.4846
2046 549.2496 1096.4846
2047 549.2496 1096.4846
2048 549.2496 1096.4846
2049 549.2496 1096.4846
2050 549.2496 1096.4846
2051 549.2496 1096.4846
2052 549.2496 1096.4846
2053 549.2496 1096.4846
2054 549.2496 1096.4846
2055 549.2496 1096.4846
2056 549.2496 1096.4846
2057 549.2496 1096.4846
2058 549.2496 1096.4846
2059 549.2496 1096.4846
2060 549.2496 1096.4846
2062 549.2496 1096.4846
2063 549.2496 1096.4846
2065 549.2496 1096.4846
2068 549.2496 1096.4846
2071 549.2496 1096.4846
2072 549.2496 1096.4846
2073 549.2496 1096.4846
2074 549.2496 1096.4846
2077 549.2496 1096.4846
2080 549.2496 1096.4846
2083 549.2496 1096.4846
2086 549.2496 1096.4846
2088 549.2496 1096.4846
2090 549.2496 1096.4846
2091 549.2496 1096.4846
2094 549.2496 1096.4846
2106 549.2496 1096.4846
2108 549.2496 1096.4846
2109 549.2496 1096.4846
2114 549.2496 1096.4846
2117 549.2496 1096.4846
2118 549.2496 1096.4846
2125 549.2496 1096.4846
2126 549.2496 1096.4846
2128 549.2496 1096.4846
2129 549.2496 1096.4846
2130 549.2496 1096.4846
2131 549.2496 1096.4846
2132 549.2496 1096.4846
2134 549.2496 1096.4846
2135 549.2496 1096.4846
2136 549.2496 1096.4846
2137 549.2496 1096.4846
2138 549.2496 1096.4846
2139 549.2496 1096.4846
2140 549.2496 1096.4846
2142 549.2496 1096.4846
2143 549.2496 1096.4846
2144 549.2496 1096.4846
2145 549.2496 1096.4846
2146 549.2496 1096.4846
2147 549.2496 1096.4846
2149 549.2496 1096.4846
2150 549.2496 1096.4846
2152 549.2496 1096.4846
2153 549.2496 1096.4846
2156 549.2496 1096.4846
2157 549.2496 1096.4846
2158 549.2496 1096.4846
2159 549.2496 1096.4846
2160 549.2496 1096.4846
2161 549.2496 1096.4846
2163 549.2496 1096.4846
2165 549.2496 1096.4846
2166 549.2496 1096.4846
2167 549.2496 1096.4846
2168 549.2496 1096.4846
2170 549.2496 1096.4846
2171 549.2496 1096.4846
2172 549.2496 1096.4846
2174 549.2496 1096.4846
2177 549.2496 1096.4846
2179 549.2496 1096.4846
2180 549.2496 1096.4846
2181 549.2496 1096.4846
2182 549.2496 1096.4846
2184 549.2496 1096.4846
2185 549.2496 1096.4846
2186 549.2496 1096.4846
2188 549.2496 1096.4846
2189 549.2496 1096.4846
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