MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 7572)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1299 481.7188 961.4231
1300 481.7188 961.4231
1301 481.7188 961.4231
1302 481.7188 961.4231
1303 481.7188 961.4231
1304 481.7188 961.4231
1305 481.7188 961.4231
1306 481.7188 961.4231
1307 481.7188 961.4231
1310 481.7188 961.4231
1311 481.7188 961.4231
1312 481.7188 961.4231
1313 481.7188 961.4231
1314 481.7188 961.4231
1317 481.7188 961.4231
1318 481.7188 961.4231
1322 481.7188 961.4231
1323 481.7188 961.4231
1325 481.7188 961.4231
1326 481.7188 961.4231
1330 481.7188 961.4231
1331 481.7188 961.4231
1332 481.7188 961.4231
1333 481.7188 961.4231
1334 481.7188 961.4231
1335 481.7188 961.4231
1337 481.7188 961.4231
1338 481.7188 961.4231
1339 481.7188 961.4231
1340 481.7188 961.4231
1341 481.7188 961.4231
1342 481.7188 961.4231
1343 481.7188 961.4231
1344 481.7188 961.4231
1346 481.7188 961.4231
1347 481.7188 961.4231
1348 481.7188 961.4231
1350 481.7188 961.4231
1351 481.7188 961.4231
1352 481.7188 961.4231
1353 481.7188 961.4231
1355 481.7188 961.4231
1358 481.7188 961.4231
1360 481.7188 961.4231
1361 495.2250 988.4354
1362 495.2250 988.4354
1363 495.2250 988.4354
1364 495.2250 988.4354
1365 495.2250 988.4354
1366 495.2250 988.4354
1367 495.2250 988.4354
1368 495.2250 988.4354
1369 495.2250 988.4354
1370 495.2250 988.4354
1371 495.2250 988.4354
1372 495.2250 988.4354
1373 495.2250 988.4354
1374 495.2250 988.4354
1375 495.2250 988.4354
1376 495.2250 988.4354
1377 495.2250 988.4354
1378 495.2250 988.4354
1379 495.2250 988.4354
1380 495.2250 988.4354
1381 495.2250 988.4354
1382 495.2250 988.4354
1388 495.2250 988.4354
1389 495.2250 988.4354
1390 495.2250 988.4354
1391 495.2250 988.4354
1393 495.2250 988.4354
1394 495.2250 988.4354
1395 495.2250 988.4354
1396 495.2250 988.4354
1397 495.2250 988.4354
1398 495.2250 988.4354
1400 495.2250 988.4354
1402 495.2250 988.4354
1403 495.2250 988.4354
1406 495.2250 988.4354 988.4284 7.11 0     +0Score > 19 indicates identity MQHTQSEK + Deamidated (NQ)
1407 495.2250 988.4354
1408 495.2250 988.4354
1409 495.2250 988.4354
1410 495.2250 988.4354
1412 495.2250 988.4354
1414 495.2250 988.4354
1415 495.2250 988.4354
1416 495.2250 988.4354
1418 495.2250 988.4354
1419 495.2250 988.4354
1421 495.2250 988.4354
1422 495.2250 988.4354
1423 495.2250 988.4354
1425 495.2250 988.4354
1428 495.2250 988.4354
1429 495.2250 988.4354
1430 495.2250 988.4354
1431 495.2250 988.4354
1432 495.2250 988.4354
1433 495.2250 988.4354
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