MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 7572)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
427 414.1881 826.3617
429 414.1881 826.3617
430 414.1881 826.3617
432 414.1881 826.3617
435 414.1881 826.3617
436 414.1881 826.3617
438 414.1881 826.3617
440 414.1881 826.3617
442 414.1881 826.3617
444 414.1881 826.3617
447 414.1881 826.3617
450 414.1881 826.3617
451 414.1881 826.3617
452 414.1881 826.3617
454 414.1881 826.3617
455 414.1881 826.3617
456 414.1881 826.3617
457 414.1881 826.3617
458 414.1881 826.3617
459 414.1881 826.3617
461 414.1881 826.3617
464 414.1881 826.3617
467 414.1881 826.3617
468 414.1881 826.3617
471 414.1881 826.3617
473 414.1881 826.3617
475 414.1881 826.3617
478 414.1881 826.3617
479 414.1881 826.3617
482 414.1881 826.3617
484 414.1881 826.3617
487 414.1881 826.3617
492 414.1881 826.3617
493 414.1881 826.3617
496 414.1881 826.3617
497 414.1881 826.3617
498 414.1881 826.3617
500 414.1881 826.3617
503 414.1881 826.3617
504 414.1881 826.3617
505 414.1881 826.3617
506 414.1881 826.3617
508 414.1881 826.3617
511 427.6943 853.3741
512 427.6943 853.3741
513 427.6943 853.3741
514 427.6943 853.3741
515 427.6943 853.3741
516 427.6943 853.3741
517 427.6943 853.3741
518 427.6943 853.3741
519 427.6943 853.3741
520 427.6943 853.3741
521 427.6943 853.3741
522 427.6943 853.3741
523 427.6943 853.3741
524 427.6943 853.3741
525 427.6943 853.3741
526 427.6943 853.3741
527 427.6943 853.3741
528 427.6943 853.3741
529 427.6943 853.3741
530 427.6943 853.3741
531 427.6943 853.3741
534 427.6943 853.3741
535 427.6943 853.3741
536 427.6943 853.3741
537 427.6943 853.3741
538 427.6943 853.3741
539 427.6943 853.3741
540 427.6943 853.3741
541 427.6943 853.3741
542 427.6943 853.3741
543 427.6943 853.3741
544 427.6943 853.3741
545 427.6943 853.3741
546 427.6943 853.3741
549 427.6943 853.3741
552 427.6943 853.3741
554 427.6943 853.3741
558 427.6943 853.3741
559 427.6943 853.3741
560 427.6943 853.3741
561 427.6943 853.3741
562 427.6943 853.3741
564 427.6943 853.3741
565 427.6943 853.3741
566 427.6943 853.3741
567 427.6943 853.3741
568 427.6943 853.3741
570 427.6943 853.3741
573 427.6943 853.3741
574 427.6943 853.3741
575 427.6943 853.3741
578 427.6943 853.3741
579 427.6943 853.3741
580 427.6943 853.3741
581 427.6943 853.3741
584 427.6943 853.3741
585 427.6943 853.3741
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