MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 7572)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
248 400.6820 799.3495
250 400.6820 799.3495
252 400.6820 799.3495
254 400.6820 799.3495
255 400.6820 799.3495
256 400.6820 799.3495
257 400.6820 799.3495
259 400.6820 799.3495
262 400.6820 799.3495
263 400.6820 799.3495
264 400.6820 799.3495
265 400.6820 799.3495
267 400.6820 799.3495
268 400.6820 799.3495
269 400.6820 799.3495
270 400.6820 799.3495
274 400.6820 799.3495
275 400.6820 799.3495
276 400.6820 799.3495
278 400.6820 799.3495
279 400.6820 799.3495
282 400.6820 799.3495
285 400.6820 799.3495
287 400.6820 799.3495
289 400.6820 799.3495
293 400.6820 799.3495
294 400.6820 799.3495
295 400.6820 799.3495
297 400.6820 799.3495
304 400.6820 799.3495
305 400.6820 799.3495
307 400.6820 799.3495
313 400.6820 799.3495
317 400.6820 799.3495
318 400.6820 799.3495
321 400.6820 799.3495
322 400.6820 799.3495
323 400.6820 799.3495
325 400.6820 799.3495
327 400.6820 799.3495
329 400.6820 799.3495
330 400.6820 799.3495
331 400.6820 799.3495
334 400.6820 799.3495
335 400.6820 799.3495
336 400.6820 799.3495
338 400.6820 799.3495
339 400.6820 799.3495
340 400.6820 799.3495
341 414.1881 826.3617
342 414.1881 826.3617
343 414.1881 826.3617
344 414.1881 826.3617
345 414.1881 826.3617
346 414.1881 826.3617
347 414.1881 826.3617
348 414.1881 826.3617
349 414.1881 826.3617
350 414.1881 826.3617
351 414.1881 826.3617
352 414.1881 826.3617
353 414.1881 826.3617
354 414.1881 826.3617
355 414.1881 826.3617
356 414.1881 826.3617
358 414.1881 826.3617
360 414.1881 826.3617
361 414.1881 826.3617
364 414.1881 826.3617
368 414.1881 826.3617
369 414.1881 826.3617
372 414.1881 826.3617
373 414.1881 826.3617
377 414.1881 826.3617
378 414.1881 826.3617
383 414.1881 826.3617
387 414.1881 826.3617
388 414.1881 826.3617
389 414.1881 826.3617
398 414.1881 826.3617
399 414.1881 826.3617
400 414.1881 826.3617
401 414.1881 826.3617
402 414.1881 826.3617
404 414.1881 826.3617
405 414.1881 826.3617
406 414.1881 826.3617
408 414.1881 826.3617
411 414.1881 826.3617
412 414.1881 826.3617
414 414.1881 826.3617
415 414.1881 826.3617
417 414.1881 826.3617
418 414.1881 826.3617
419 414.1881 826.3617
421 414.1881 826.3617
422 414.1881 826.3617
423 414.1881 826.3617
424 414.1881 826.3617
425 414.1881 826.3617
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