MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | test |
| MS data file | : | PRT1346_ENIGMA_14.mgf |
| Database | : | B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues) |
| Timestamp | : | 4 Dec 2025 at 15:32:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 7,621 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | tr|Q65KZ6|Q65KZ6_BACLD | 22 | Glutathione hydrolase proenzyme OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=ggt PE=1 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | sp|Q65NA8|Y2808_BACLD | 21 | Putative hydro-lyase BLi00500/BL02808 OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BLi00500 PE=3 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | tr|Q65P32|Q65P32_BACLD | 21 | HAD-superfamily hydrolase, subfamily IA, variant 1 YfnB OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=yfnB PE=4 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | sp|Q65GS7|YQGF_BACLD | 19 | Putative pre-16S rRNA nuclease OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BLi02867 PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | tr|Q65JU2|Q65JU2_BACLD | 18 | Uncharacterized protein OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BL02281 PE=4 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | tr|Q65JB9|Q65JB9_BACLD | 16 | YraE OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=yraE PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | tr|Q65JB9|Q65JB9_BACLD |
16 | 7629 | 33 (1) | 1 (1) | 0.46 | |
| YraE OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=yraE PE=4 SV=1 | |||||||
33 peptide matches (5 non-duplicate, 28 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 218 |
18 |
400.6820 | 1598.6989 | 1598.6916 | 4.57 | 0 | 16 | 0.054 | 1Score > 16 indicates identity |
U | K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M) |
| 258 |
3 |
400.6820 | 1598.6989 | 1598.6916 | 4.57 | 0 | 8 | 0.38 | 1Score > 16 indicates identity |
U | K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M) |
| 271 |
3 |
400.6820 | 1598.6989 | 1598.6916 | 4.57 | 0 | 12 | 0.15 | 1Score > 16 indicates identity |
U | K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M) |
| 266 |
400.6820 | 1598.6989 | 1598.6916 | 4.57 | 0 | (2) | 1.4 | 2Score > 16 indicates identity |
U | K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M) | |
| 284 |
400.6820 | 1598.6989 | 1598.6916 | 4.57 | 0 | (4) | 0.86 | 1Score > 16 indicates identity |
U | K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M) | |
| 319 |
400.6820 | 1598.6989 | 1598.6916 | 4.57 | 0 | (6) | 0.58 | 1Score > 16 indicates identity |
U | K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M) | |
| 5150 |
792.3600 | 1582.7055 | 1582.6967 | 5.58 | 0 | 1 | 1 | 1Score > 20 indicates identity Score > 14 indicates homology |
U | K.SSTMTGMVQDQELR.D + Deamidated (NQ) | |
| 5153 |
4 |
792.3600 | 1582.7055 | 1582.6967 | 5.58 | 0 | 7 | 0.61 | 1Score > 20 indicates identity Score > 17 indicates homology |
U | K.SSTMTGMVQDQELR.D + Deamidated (NQ) |
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