MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : B-licheniformis 20211213 (4,284 sequences; 1,240,737 residues)
Timestamp : 4 Dec 2025 at 15:32:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–6 (out of 6)

Page: 1 

+1

Accession Score Description
1 tr|Q65KZ6|Q65KZ6_BACLD 22 Glutathione hydrolase proenzyme OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=ggt PE=1 SV=1

+2

Accession Score Description
1 sp|Q65NA8|Y2808_BACLD 21 Putative hydro-lyase BLi00500/BL02808 OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BLi00500 PE=3 SV=1

+3

Accession Score Description
1 tr|Q65P32|Q65P32_BACLD 21 HAD-superfamily hydrolase, subfamily IA, variant 1 YfnB OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=yfnB PE=4 SV=1

+4

Accession Score Description
1 sp|Q65GS7|YQGF_BACLD 19 Putative pre-16S rRNA nuclease OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BLi02867 PE=3 SV=1

+5

Accession Score Description
1 tr|Q65JU2|Q65JU2_BACLD 18 Uncharacterized protein OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BL02281 PE=4 SV=1

-6

Accession Score Description
1 tr|Q65JB9|Q65JB9_BACLD 16 YraE OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=yraE PE=4 SV=1
Score Mass Matches Sequences emPAI
6.1 tr|Q65JB9|Q65JB9_BACLD 16 7629 33 (1) 1 (1) 0.46
YraE OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=yraE PE=4 SV=1

-33 peptide matches (5 non-duplicate, 28 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
218 -18 400.6820 1598.6989 1598.6916 4.57 0 16 0.054 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
191   400.6820 1598.6989 1598.6916 4.57 0 (1) 1.6 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
195   400.6820 1598.6989 1598.6916 4.57 0 (2) 1.5 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
197   400.6820 1598.6989 1598.6916 4.57 0 (0) 2.1 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
198   400.6820 1598.6989 1598.6916 4.57 0 (10) 0.25 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
199   400.6820 1598.6989 1598.6916 4.57 0 (2) 1.5 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
206   400.6820 1598.6989 1598.6916 4.57 0 (0) 2 +4Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
209   400.6820 1598.6989 1598.6916 4.57 0 (0) 2.1 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
214   400.6820 1598.6989 1598.6916 4.57 0 (5) 0.73 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
216   400.6820 1598.6989 1598.6916 4.57 0 (4) 0.9 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
219   400.6820 1598.6989 1598.6916 4.57 0 (1) 1.8 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
228   400.6820 1598.6989 1598.6916 4.57 0 (0) 2.1 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
230   400.6820 1598.6989 1598.6916 4.57 0 (8) 0.38 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
232   400.6820 1598.6989 1598.6916 4.57 0 (2) 1.3 +2Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
233   400.6820 1598.6989 1598.6916 4.57 0 (3) 1.1 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
277   400.6820 1598.6989 1598.6916 4.57 0 (0) 2.2 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
291   400.6820 1598.6989 1598.6916 4.57 0 (7) 0.44 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
298   400.6820 1598.6989 1598.6916 4.57 0 (1) 1.7 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
316   400.6820 1598.6989 1598.6916 4.57 0 (2) 1.5 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
258 +3 400.6820 1598.6989 1598.6916 4.57 0 8 0.38 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
271 +3 400.6820 1598.6989 1598.6916 4.57 0 12 0.15 +1Score > 16 indicates identity U K.SSTMTGMVQDQELR.D + Deamidated (NQ); Oxidation (M)
5150   792.3600 1582.7055 1582.6967 5.58 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology		 U K.SSTMTGMVQDQELR.D + Deamidated (NQ)
5153 +4 792.3600 1582.7055 1582.6967 5.58 0 7 0.61 +1Score > 20 indicates identity
Score > 17 indicates homology		 U K.SSTMTGMVQDQELR.D + Deamidated (NQ)
Page: 1 

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