MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 7101–7200 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
7058 940.9276 1879.8405
7059 940.9276 1879.8405
7061 940.9276 1879.8405
7063 940.9276 1879.8405
7064 940.9276 1879.8405
7065 940.9276 1879.8405
7066 940.9276 1879.8405
7067 940.9276 1879.8405
7068 940.9276 1879.8405
7069 940.9276 1879.8405
7070 940.9276 1879.8405
7071 940.9276 1879.8405
7072 940.9276 1879.8405
7073 940.9276 1879.8405
7074 940.9276 1879.8405
7075 940.9276 1879.8405
7076 940.9276 1879.8405
7077 940.9276 1879.8405
7078 940.9276 1879.8405
7079 940.9276 1879.8405
7080 940.9276 1879.8405
7081 940.9276 1879.8405
7082 940.9276 1879.8405
7083 940.9276 1879.8405
7084 940.9276 1879.8405
7085 940.9276 1879.8405
7087 940.9276 1879.8405
7088 940.9276 1879.8405
7089 940.9276 1879.8405
7090 940.9276 1879.8405
7091 940.9276 1879.8405
7092 940.9276 1879.8405
7093 940.9276 1879.8405
7094 940.9276 1879.8405
7095 940.9276 1879.8405
7096 940.9276 1879.8405
7097 940.9276 1879.8405
7098 940.9276 1879.8405
7099 940.9276 1879.8405
7100 940.9276 1879.8405
7101 940.9276 1879.8405
7102 940.9276 1879.8405
7103 940.9276 1879.8405
7104 940.9276 1879.8405
7105 940.9276 1879.8405
7106 940.9276 1879.8405
7107 940.9276 1879.8405
7108 940.9276 1879.8405
7109 940.9276 1879.8405
7110 940.9276 1879.8405
7111 940.9276 1879.8405
7112 940.9276 1879.8405
7113 940.9276 1879.8405
7114 940.9276 1879.8405
7115 954.4337 1906.8529
7116 954.4337 1906.8529
7117 954.4337 1906.8529
7118 954.4337 1906.8529
7119 954.4337 1906.8529
7120 954.4337 1906.8529
7121 954.4337 1906.8529
7122 954.4337 1906.8529
7123 954.4337 1906.8529
7124 954.4337 1906.8529
7125 954.4337 1906.8529
7126 954.4337 1906.8529
7127 954.4337 1906.8529
7129 954.4337 1906.8529
7130 954.4337 1906.8529
7131 954.4337 1906.8529
7132 954.4337 1906.8529
7133 954.4337 1906.8529
7134 954.4337 1906.8529
7136 954.4337 1906.8529
7138 954.4337 1906.8529
7139 954.4337 1906.8529
7140 954.4337 1906.8529
7141 954.4337 1906.8529
7142 954.4337 1906.8529
7143 954.4337 1906.8529
7144 954.4337 1906.8529
7145 954.4337 1906.8529
7148 954.4337 1906.8529
7150 954.4337 1906.8529
7154 954.4337 1906.8529
7157 954.4337 1906.8529
7159 954.4337 1906.8529
7164 954.4337 1906.8529
7166 954.4337 1906.8529
7169 954.4337 1906.8529
7170 954.4337 1906.8529
7171 954.4337 1906.8529
7172 954.4337 1906.8529
7173 954.4337 1906.8529
7174 954.4337 1906.8529
7175 954.4337 1906.8529
7176 954.4337 1906.8529
7177 954.4337 1906.8529
7180 954.4337 1906.8529
7182 954.4337 1906.8529
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