MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 7001–7100 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6944 927.4214 1852.8283
6945 927.4214 1852.8283
6946 940.9276 1879.8405
6948 940.9276 1879.8405
6949 940.9276 1879.8405
6950 940.9276 1879.8405
6951 940.9276 1879.8405
6952 940.9276 1879.8405
6953 940.9276 1879.8405
6954 940.9276 1879.8405
6955 940.9276 1879.8405
6956 940.9276 1879.8405
6959 940.9276 1879.8405
6960 940.9276 1879.8405
6961 940.9276 1879.8405
6962 940.9276 1879.8405
6964 940.9276 1879.8405
6965 940.9276 1879.8405
6966 940.9276 1879.8405
6967 940.9276 1879.8405
6969 940.9276 1879.8405
6970 940.9276 1879.8405
6971 940.9276 1879.8405
6972 940.9276 1879.8405
6973 940.9276 1879.8405
6974 940.9276 1879.8405
6975 940.9276 1879.8405
6977 940.9276 1879.8405
6978 940.9276 1879.8405
6979 940.9276 1879.8405
6981 940.9276 1879.8405
6982 940.9276 1879.8405
6983 940.9276 1879.8405
6984 940.9276 1879.8405
6986 940.9276 1879.8405
6987 940.9276 1879.8405
6988 940.9276 1879.8405
6989 940.9276 1879.8405
6990 940.9276 1879.8405
6991 940.9276 1879.8405
6992 940.9276 1879.8405
6993 940.9276 1879.8405
6994 940.9276 1879.8405
6995 940.9276 1879.8405
6996 940.9276 1879.8405
6997 940.9276 1879.8405
6998 940.9276 1879.8405
6999 940.9276 1879.8405
7000 940.9276 1879.8405
7001 940.9276 1879.8405
7002 940.9276 1879.8405
7003 940.9276 1879.8405
7004 940.9276 1879.8405
7005 940.9276 1879.8405
7006 940.9276 1879.8405
7007 940.9276 1879.8405
7008 940.9276 1879.8405
7009 940.9276 1879.8405
7010 940.9276 1879.8405
7011 940.9276 1879.8405
7012 940.9276 1879.8405
7013 940.9276 1879.8405
7014 940.9276 1879.8405
7015 940.9276 1879.8405
7016 940.9276 1879.8405
7017 940.9276 1879.8405
7018 940.9276 1879.8405
7019 940.9276 1879.8405
7020 940.9276 1879.8405
7021 940.9276 1879.8405
7022 940.9276 1879.8405
7023 940.9276 1879.8405
7024 940.9276 1879.8405
7025 940.9276 1879.8405
7026 940.9276 1879.8405
7027 940.9276 1879.8405
7028 940.9276 1879.8405
7029 940.9276 1879.8405
7030 940.9276 1879.8405
7031 940.9276 1879.8405
7032 940.9276 1879.8405
7033 940.9276 1879.8405
7034 940.9276 1879.8405
7036 940.9276 1879.8405
7037 940.9276 1879.8405
7038 940.9276 1879.8405
7040 940.9276 1879.8405
7041 940.9276 1879.8405
7042 940.9276 1879.8405
7043 940.9276 1879.8405
7044 940.9276 1879.8405
7045 940.9276 1879.8405
7046 940.9276 1879.8405
7048 940.9276 1879.8405
7050 940.9276 1879.8405
7051 940.9276 1879.8405
7053 940.9276 1879.8405
7054 940.9276 1879.8405
7056 940.9276 1879.8405
7057 940.9276 1879.8405
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