MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6901–7000 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6811 927.4214 1852.8283
6812 927.4214 1852.8283
6813 927.4214 1852.8283
6815 927.4214 1852.8283
6817 927.4214 1852.8283
6819 927.4214 1852.8283
6822 927.4214 1852.8283
6823 927.4214 1852.8283
6824 927.4214 1852.8283
6825 927.4214 1852.8283
6826 927.4214 1852.8283
6828 927.4214 1852.8283
6830 927.4214 1852.8283
6831 927.4214 1852.8283
6833 927.4214 1852.8283
6835 927.4214 1852.8283
6838 927.4214 1852.8283
6839 927.4214 1852.8283
6841 927.4214 1852.8283
6842 927.4214 1852.8283
6844 927.4214 1852.8283
6845 927.4214 1852.8283
6846 927.4214 1852.8283
6847 927.4214 1852.8283
6848 927.4214 1852.8283
6852 927.4214 1852.8283
6853 927.4214 1852.8283
6856 927.4214 1852.8283
6857 927.4214 1852.8283
6858 927.4214 1852.8283
6859 927.4214 1852.8283
6860 927.4214 1852.8283
6861 927.4214 1852.8283
6862 927.4214 1852.8283
6863 927.4214 1852.8283
6864 927.4214 1852.8283
6865 927.4214 1852.8283
6868 927.4214 1852.8283
6871 927.4214 1852.8283
6872 927.4214 1852.8283
6873 927.4214 1852.8283
6874 927.4214 1852.8283
6875 927.4214 1852.8283
6876 927.4214 1852.8283
6877 927.4214 1852.8283
6878 927.4214 1852.8283
6879 927.4214 1852.8283
6880 927.4214 1852.8283
6883 927.4214 1852.8283
6884 927.4214 1852.8283
6885 927.4214 1852.8283
6886 927.4214 1852.8283
6887 927.4214 1852.8283
6888 927.4214 1852.8283
6889 927.4214 1852.8283
6890 927.4214 1852.8283
6891 927.4214 1852.8283
6892 927.4214 1852.8283
6893 927.4214 1852.8283
6894 927.4214 1852.8283
6895 927.4214 1852.8283
6896 927.4214 1852.8283
6897 927.4214 1852.8283
6899 927.4214 1852.8283
6900 927.4214 1852.8283
6901 927.4214 1852.8283
6903 927.4214 1852.8283
6904 927.4214 1852.8283
6905 927.4214 1852.8283
6906 927.4214 1852.8283
6907 927.4214 1852.8283
6908 927.4214 1852.8283
6910 927.4214 1852.8283
6911 927.4214 1852.8283
6912 927.4214 1852.8283
6913 927.4214 1852.8283
6914 927.4214 1852.8283
6915 927.4214 1852.8283
6916 927.4214 1852.8283
6918 927.4214 1852.8283
6921 927.4214 1852.8283
6922 927.4214 1852.8283
6924 927.4214 1852.8283
6925 927.4214 1852.8283
6926 927.4214 1852.8283
6927 927.4214 1852.8283
6928 927.4214 1852.8283
6929 927.4214 1852.8283
6930 927.4214 1852.8283
6932 927.4214 1852.8283
6933 927.4214 1852.8283
6935 927.4214 1852.8283
6936 927.4214 1852.8283
6937 927.4214 1852.8283
6938 927.4214 1852.8283
6939 927.4214 1852.8283
6940 927.4214 1852.8283
6941 927.4214 1852.8283
6942 927.4214 1852.8283
6943 927.4214 1852.8283
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