MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6801–6900 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6693 913.9153 1825.8160
6694 913.9153 1825.8160
6695 913.9153 1825.8160
6696 913.9153 1825.8160
6697 913.9153 1825.8160
6698 913.9153 1825.8160
6699 913.9153 1825.8160
6700 913.9153 1825.8160
6701 913.9153 1825.8160
6702 913.9153 1825.8160
6703 913.9153 1825.8160
6706 913.9153 1825.8160
6711 913.9153 1825.8160
6714 913.9153 1825.8160
6715 913.9153 1825.8160
6717 913.9153 1825.8160
6718 913.9153 1825.8160
6719 913.9153 1825.8160
6720 913.9153 1825.8160
6721 913.9153 1825.8160
6722 913.9153 1825.8160
6723 913.9153 1825.8160
6724 913.9153 1825.8160
6725 913.9153 1825.8160
6726 913.9153 1825.8160
6727 913.9153 1825.8160
6728 913.9153 1825.8160
6729 913.9153 1825.8160
6730 913.9153 1825.8160
6731 913.9153 1825.8160
6732 913.9153 1825.8160
6733 913.9153 1825.8160
6734 913.9153 1825.8160
6735 913.9153 1825.8160
6736 913.9153 1825.8160
6737 913.9153 1825.8160
6738 913.9153 1825.8160
6739 913.9153 1825.8160
6740 913.9153 1825.8160
6741 913.9153 1825.8160
6742 913.9153 1825.8160
6743 913.9153 1825.8160
6744 913.9153 1825.8160
6745 913.9153 1825.8160
6746 913.9153 1825.8160
6747 913.9153 1825.8160
6748 913.9153 1825.8160
6749 913.9153 1825.8160
6750 913.9153 1825.8160
6751 913.9153 1825.8160
6752 913.9153 1825.8160
6753 913.9153 1825.8160
6754 913.9153 1825.8160
6755 913.9153 1825.8160
6756 913.9153 1825.8160
6757 913.9153 1825.8160
6758 913.9153 1825.8160
6759 913.9153 1825.8160
6761 913.9153 1825.8160
6762 913.9153 1825.8160
6763 913.9153 1825.8160
6765 913.9153 1825.8160
6766 913.9153 1825.8160
6767 913.9153 1825.8160
6768 913.9153 1825.8160
6769 913.9153 1825.8160
6770 913.9153 1825.8160
6771 913.9153 1825.8160
6772 913.9153 1825.8160
6773 913.9153 1825.8160
6774 913.9153 1825.8160
6775 913.9153 1825.8160
6776 913.9153 1825.8160
6777 927.4214 1852.8283
6778 927.4214 1852.8283
6779 927.4214 1852.8283
6780 927.4214 1852.8283
6781 927.4214 1852.8283
6782 927.4214 1852.8283
6783 927.4214 1852.8283
6784 927.4214 1852.8283
6785 927.4214 1852.8283
6786 927.4214 1852.8283
6787 927.4214 1852.8283
6788 927.4214 1852.8283
6789 927.4214 1852.8283
6790 927.4214 1852.8283
6791 927.4214 1852.8283
6792 927.4214 1852.8283
6793 927.4214 1852.8283
6796 927.4214 1852.8283
6798 927.4214 1852.8283
6800 927.4214 1852.8283
6801 927.4214 1852.8283
6803 927.4214 1852.8283
6804 927.4214 1852.8283
6807 927.4214 1852.8283
6808 927.4214 1852.8283
6809 927.4214 1852.8283
6810 927.4214 1852.8283
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