MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : test
MS data file : PRT1346_ENIGMA_14.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 4 Dec 2025 at 15:28:39 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,621

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6701–6800 (out of 7566)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6569 900.4092 1798.8038
6570 900.4092 1798.8038
6571 900.4092 1798.8038
6572 900.4092 1798.8038
6573 900.4092 1798.8038
6574 900.4092 1798.8038
6575 900.4092 1798.8038
6576 900.4092 1798.8038
6577 900.4092 1798.8038
6578 900.4092 1798.8038
6579 900.4092 1798.8038
6580 900.4092 1798.8038
6581 900.4092 1798.8038
6582 900.4092 1798.8038
6583 900.4092 1798.8038
6584 900.4092 1798.8038
6585 900.4092 1798.8038
6586 900.4092 1798.8038
6587 900.4092 1798.8038
6589 900.4092 1798.8038
6590 900.4092 1798.8038
6591 900.4092 1798.8038
6592 900.4092 1798.8038
6594 900.4092 1798.8038
6596 900.4092 1798.8038
6597 900.4092 1798.8038
6599 900.4092 1798.8038
6600 900.4092 1798.8038
6601 900.4092 1798.8038
6603 900.4092 1798.8038
6605 900.4092 1798.8038
6606 900.4092 1798.8038
6607 900.4092 1798.8038
6608 913.9153 1825.8160
6609 913.9153 1825.8160
6610 913.9153 1825.8160
6611 913.9153 1825.8160
6612 913.9153 1825.8160
6613 913.9153 1825.8160
6614 913.9153 1825.8160
6615 913.9153 1825.8160
6616 913.9153 1825.8160
6617 913.9153 1825.8160
6619 913.9153 1825.8160
6620 913.9153 1825.8160
6621 913.9153 1825.8160
6622 913.9153 1825.8160
6625 913.9153 1825.8160
6626 913.9153 1825.8160
6632 913.9153 1825.8160
6634 913.9153 1825.8160
6635 913.9153 1825.8160
6637 913.9153 1825.8160
6639 913.9153 1825.8160
6641 913.9153 1825.8160
6643 913.9153 1825.8160
6644 913.9153 1825.8160
6645 913.9153 1825.8160
6646 913.9153 1825.8160
6647 913.9153 1825.8160
6648 913.9153 1825.8160
6649 913.9153 1825.8160
6650 913.9153 1825.8160
6651 913.9153 1825.8160
6652 913.9153 1825.8160
6653 913.9153 1825.8160
6655 913.9153 1825.8160
6656 913.9153 1825.8160
6657 913.9153 1825.8160
6660 913.9153 1825.8160
6661 913.9153 1825.8160
6662 913.9153 1825.8160
6663 913.9153 1825.8160
6664 913.9153 1825.8160
6665 913.9153 1825.8160
6666 913.9153 1825.8160
6667 913.9153 1825.8160
6668 913.9153 1825.8160
6669 913.9153 1825.8160
6670 913.9153 1825.8160
6672 913.9153 1825.8160
6673 913.9153 1825.8160
6674 913.9153 1825.8160
6675 913.9153 1825.8160
6677 913.9153 1825.8160
6678 913.9153 1825.8160
6679 913.9153 1825.8160
6680 913.9153 1825.8160
6681 913.9153 1825.8160
6682 913.9153 1825.8160
6683 913.9153 1825.8160
6684 913.9153 1825.8160
6685 913.9153 1825.8160
6686 913.9153 1825.8160
6687 913.9153 1825.8160
6688 913.9153 1825.8160
6689 913.9153 1825.8160
6690 913.9153 1825.8160
6691 913.9153 1825.8160
6692 913.9153 1825.8160
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